Results 61 to 70 of about 14,674 (226)

Biochemical characterization of the two novel mgCas12a proteins from the human gut metagenome

Scientific Reports, 2022
CRISPR/Cas9 and Cas12a belonging to the Class II CRISPR system are characterized by a single-component effector protein. Despite unique features of Cas12a like DNA cleavage with 5′ staggered ends and a single crRNA, Cas12a has not been adopted in ...
Han Seong Kim, Dong-wook Kim, Sungjin Kim, Sunghwa Choe   +3 more
doaj   +1 more source

Ionic Gating of Liquid‐Crystal‐Like Pteridine Assemblies Enables Tunable Light Scattering

Advanced Materials, EarlyView.
Tunable optical properties emerge through developmentally regulated ionic gating, which transforms disordered, ultraviolet (UV)‐absorbing organelles into concentrically ordered, liquid‐crystal‐like light scatterers. By integrating analyses of pteridine composition, potassium‐dependent assembly, hierarchical ultrastructure, and optical behavior, the ...
Sourabh Bera, Zohar Eyal, Yael Noy, Yuval Barzilay, Siddharth Sahoo, Srikant Moharana, Avi Baram, Tali Lerer‐Goldshtein, Neta Varsano, Bar Bader, Lothar Houben, Uwe Heinig, Iddo Pinkas, Moshe Goldsmith, Tamar Unger, Shira Albeck, Andrea Sorrentino, Maxim Itkin, Sergey Malitsky, Ziv Porat, Ido Hadar, Venkata Jayasurya Yallapragada, Dvir Gur   +22 more
wiley   +1 more source

Targeting of CRISPR-Cas12a crRNAs into human mitochondria

Biochimie
Mitochondrial gene editing holds great promise as a therapeutic approach for mitochondrial diseases caused by mutations in the mitochondrial DNA (mtDNA). Current strategies focus on reducing mutant mtDNA heteroplasmy levels through targeted cleavage or base editing. However, the delivery of editing components into mitochondria remains a challenge. Here
Nikitchina, Natalia, Ulashchik, Egor, Shmanai, Vadim, Heckel, Anne-Marie, Tarassov, Ivan, Mazunin, Ilya, Entelis, Nina   +6 more
openaire   +3 more sources

Magnetically Actuated Microrobot for Drug Delivery: Current Progress and Challenges

Advanced Robotics Research, EarlyView.
Magnetically actuated microrobots are reshaping targeted drug delivery through wireless propulsion, programmable materials, and localized therapeutic release. This review examines how fabrication, body material selection, structural architecture, loading mechanisms, and imaging‐guided control converge to enable microrobotic therapies while identifying ...
Yang Yang, David J. Cappelleri
wiley   +1 more source

Engineering CRISPR–Cpf1 crRNAs and mRNAs to maximize genome editing efficiency [PDF]

Nature Biomedical Engineering, 2017
Cpf1, a type-V CRISPR-Cas effector endonuclease, exhibits gene-editing activity in human cells through a single RNA-guided approach. Here, we report the design and assessment of an array of 42 types of engineered Acidaminococcus sp. Cpf1 (AsCpf1) CRISPR RNAs (crRNAs) and 5 types of AsCpf1 mRNAs, and show that the top-performing modified crRNA (cr3'5F ...
Bin Li, Weiyu Zhao, Xiao Luo, Xinfu Zhang, Chenglong Li, Chunxi Zeng, Yizhou Dong   +6 more
openaire   +2 more sources

A detailed comparison of the interactions of SeCsm4 and ToCsm4 with crRNA.

, 2023
(A) SeCsm4 residues forming interactions with the crRNA 5’-tag that are likely critical for recognition shown as sticks. Three residues that form van der Waals interaction with crRNA and sterically position it are shown as spheres.
Asma Hatoum-Aslan (4451431), Jack A. Dunkle (2091862), Scott M. Stagg (1281813), Mohammadreza Paraan (16436961), Lucy Chou-Zheng (16436964), Sarah A. Khweis (14173444), Allyn J. Schoeffler (16436967), Mohamed Nasef (14173441)   +7 more
core   +1 more source

CRISPR RNA biogenesis by a Cas6 nuclease [PDF]

, 2015
Clustered regularly interspaced short palindromic repeats (CRISPRs) and associated (Cas) proteins form the basis of a prokaryotic adaptive immune system.
Sokolowski, Richard D.
core   +2 more sources

Optimization of CRISPR/LbCas12a-mediated gene editing in Arabidopsis.

PLoS ONE, 2022
CRISPR/LbCas12a system (LbCpf1) has been widely used for genome modification including plant species. However, the efficiency of CRISPR/LbCas12a varied considerably in different plant species and tissues, and the editing efficiency needs to be further ...
Qiang Zhang, Yan Zhang, Yiping Chai
doaj   +1 more source

Attomolar Detection of HIV‐1 With Label‐Free RCA‐rCRISPR on Smartphone

Advanced Science, EarlyView.
A new diagnostic strategy integrating rolling circle amplification (RCA) with a plasmid reporter–based ratiometric CRISPR (rCRISPR) system was developed to detect unfragmented HIV genomes with single‐digit attomolar sensitivity. The method quantifies targets using the plasmid relaxation ratio and employs a smartphone‐based mini‐gel device to enable ...
Noor Mohammad, Anastasiia Steksova, Yuyang Tang, Leyao Huang, Alireza Velayati, Shengwei Zhang, Aditi Dey Poonam, Sina Jamalzadegan, Matthew Breen, Hongjie Chen, Mahsa Bagi, Guochun Jiang, Qingshan Wei   +12 more
wiley   +1 more source

Genetic Surgery: The Revolutionary CRISPR-Cas9 Scissors [PDF]

Journal of Basic Research in Medical Sciences
Introduction: The CRISPR-Cas9 system, a revolutionary genome-editing tool which stems from a bacterial adaptive immune system, shows a paradigm shift in designing new therapeutics in medicine.
Elahe Alivaisi, Ali Noori-Zadeh
doaj