Results 81 to 90 of about 14,674 (226)

Transcriptional analysis of CRISPR I-B arrays of Leptospira interrogans serovar Lai and its processing by Cas6

Frontiers in Microbiology, 2022
In the genome of various Leptospira interrogans serovars, the subtype I-B locus of CRISPR-Cas possesses either one or multiple CRISPR arrays. In silico database (CRISPRCasdb) for predicting CRISPR-Cas reveals seven CRISPR arrays in L. interrogans serovar
Aman Prakash, Manish Kumar
doaj   +1 more source

Harnessing noncanonical crRNAs to improve functionality of Cas12a orthologs

Cell Reports
There is a broad diversity among Cas12a endonucleases that possess nucleic acid detection and gene-editing capabilities, but few are studied extensively. Here, we present an exhaustive investigation of 23 Cas12a orthologs, with a focus on their cis- and trans-cleavage activities in combination with noncanonical crRNAs.
Long T. Nguyen, Nicolas C. Macaluso, Noah R. Rakestraw, Dylan R. Carman, Brianna L.M. Pizzano, Raymond C. Hautamaki, Santosh R. Rananaware, Isabel E. Roberts, Piyush K. Jain   +8 more
openaire   +3 more sources

Facilitating crRNA Design by Integrating DNA Interaction Features of CRISPR‐Cas12a System

Advanced Science
The CRISPR‐Cas12a system has gained significant attention as a rapid nucleic acid diagnostic tool due to its crRNA‐guided trans‐cleavage activity. Accurately predicting the activity of different targets is significant to facilitate the crRNA availability
Zhihao Yao, Wanglu Li, Kaiyu He, Hongmei Wang, Yan Xu, Qun Wu, Liu Wang, Yao Nie   +7 more
doaj   +1 more source

Targeted RNA knockdown by crRNA guided Csm in zebrafish [PDF]

, 2017
ABSTRACT RNA interference (RNAi) is a powerful experimental tool for RNA knockdown, but not all organisms are amenable. Here, we provide a “proof of principle” demonstration that CRISPR endoribonucleases can be used for programmable mRNA transcript degradation.
Fricke, Thomas, Tamulaitis, Gintautas, Smalakyte, Dalia, Pastor, Michal, Kolano, Agnieszka, Siksnys, Virginijus, Bochtler, Matthias   +6 more
openaire   +1 more source

Various relationships among crRNA and siRNA screen data.

, 2016
(A) Biological replicates of a synthetic CRISPR screening experiment. Each dot indicates the value derived from a single crRNA assayed in separate experiments. (B) Increased nuclear area exhibits a strong negative correlation with nuclear count.
Jenille Tan (3605864), Scott E. Martin (30985)   +1 more
core   +1 more source

Negative staining analysis of apo Lb2Cas12a and Lb2Cas12a-crRNA.

, 2023
(A) Left, representative raw negative staining micrograph and 2D class averages of apo Lb2Cas12a particles. Right, representative raw negative staining micrograph and 2D class averages of Lb2Cas12a-crRNA particles (B) apo Lb2Cas12a and Lb2Cas12a-crRNA ...
Sun Meng (11007860), Shi Jian (14790680), Yuren Adam Yuan (14790683), Chacko Jobichen (43238), Satoru Machida (14790671), J. Sivaraman (31440), Li Jianwei (14790668), Randy J. Read (14790674), Chen Hongying (14790677)   +8 more
core   +1 more source

Engineering Biology Beyond Single Genes: Advances and Challenges in Multiplex Genome Editing

Animal Research and One Health, EarlyView.
Multiplex genome editing is transforming genome engineering from single‐gene perturbation to network‐level control, yet its broader application remains limited by challenges in gRNA array engineering, delivery technologies, and safety management. Emerging AI‐driven approaches are accelerating guide RNA design and CRISPR effector optimization for ...
Linli Wang, Yongbin Liu, Hongbing Han
wiley   +1 more source

TopCas: Topology‐Gated Cas12a via DNA‐RNA Chimeric Circular crRNA for Amplification‐Free Nucleic Acid Detection and Conditional Gene Editing

Advanced Science
Controlled activation of CRISPR‐Cas12a is critical for achieving conditional gene editing and molecular diagnostics. As an indispensable component for forming an active complex, CRISPR RNA (crRNA) represents a key route to regulate Cas12a activity. Here,
Shun Zhang, Wen Sun, Ting Xiao, Yali Wang, Xianlan Wu, Huiyou Chen, Ming Chen, Jun Zhang   +7 more
doaj   +1 more source

The β integrin modulates serotonin sensitivity via NPxY motifs to regulate egg laying and mechanosensation behaviors in Caenorhabditis elegans

Developmental Dynamics, EarlyView.
Abstract Background Integrin is an αβ heterodimeric receptor to the extracellular matrix; its binding to the matrix recruits focal adhesions to two NPxY motifs, the tyrosine phosphorylation sites in the cytoplasmic domain. Studies found that replacing tyrosines (Y) with phenylalanines (F) in the motif of β1 integrin displayed little developmental or ...
Josh Haram Bumm, Daniel Dogeon Lee, Zhongqiang Qiu, Angela Haeun Choi, Meghana Kolluri, Michael J. Olsen, Lianzijun Wang, Myeongwoo Lee   +7 more
wiley   +1 more source

Functional decoupling of crRNA enables customizable CRISPR diagnostics

Nucleic Acids Research
Abstract One-pot CRISPR-based diagnostics have transformed nucleic acid testing, yet their design customizability remains constrained. Because target programming and cis-cleavage activity are simultaneously determined during CRISPR RNA (crRNA) design, optimizing cleavage activity to match isothermal amplification inevitably requires ...
Hyungbin Park, Jiyoung Yun, Kyuhan Lee, Jee Hyeon Kim, Ji-Ho Park, Yeo-Jin Park, Jun Hyeok Park, Hoyeon Lee, Min-Gon Kim   +8 more
openaire   +2 more sources