Results 101 to 110 of about 14,674 (226)
Frontiers in MicrobiologyThe CRISPR-Cas system, an adaptive immune mechanism in prokaryotes against bacteriophages, has been developed into a versatile tool for recognizing and cleaving target nucleic acid sequences.
Chan Kyeong Lee +3 more
doaj +1 more source
Neuroprotection, EarlyView.Abstract Prime editing, a novel clustered regularly interspaced short palindromic repeats (CRISPR)‐based technology, fuses a reverse transcriptase (RT) to an engineered CRISPR‐associated protein 9 (Cas9) and uses a prime editing guide RNA (pegRNA)‐encoded template.
Tianshan Ji +4 more
wiley +1 more source
Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence
Scientific Reports, 2020 AbstractCRISPR/Cas-mediated genome editing is a powerful tool for generating genetically mutated cells and organisms. Linearisation of donor cassettes with this system has been shown to facilitate both transgene donor insertion and targeted knock-in. Here, we developed a donor plasmid that we name pCriMGET (plasmid of syntheticCRISPR coded RNA target ...
Ishibashi, Riki +5 more
openaire +3 more sources
Structural homologs of Lb2Cas12a-crRNA from the DALI server (Top 20).
, 2023 Structural homologs of Lb2Cas12a-crRNA from the DALI server (Top 20).
Sun Meng (11007860) +8 more
core +1 more source
Pest Management Science, Volume 81, Issue 4, Page 1988-1999, April 2025.Schematic diagram of the single and dual RPA‐CRISPR/Cas12a/13a diagnostic assays for the detection of Pantoea stewartii subsp. stewartii and Maize dwarf mosaic virus. The validated assays provide a useful and sensitive molecular tool for detecting two quarantine pathogens of maize within a minimal resource framework suitable for fast‐tracking the ...
Qian Tian +6 more
wiley +1 more source
British Journal of Haematology, EarlyView.Summary While the majority of myeloid neoplasms are sporadic, the increasing application of germline genetic testing has led the World Health Organization to designate ‘Myeloid malignancies with germline predisposition’ as a distinct clinical entity, carrying major implications for clinical care and research.
Elena Vuelta +9 more
wiley +1 more source
, 2019 Figure S1. Phylogeny tree of non-redundant Cas12a orthologs and selected Cas12a loci for genome editing. Figure S2. In vitro DNA cleavage assay for Cas12a PAM sequences. Figure S3. Six new Cas12a proteins mediated robust genome editing in mammalian cells.
Kai Xu (122914) +10 more
core +1 more source
Cell Proliferation, EarlyView.Histone modifications at CRISPR–Cas9‐induced DNA double‐strand breaks shape chromatin states and regulate DNA repair pathway choice. Transient epigenetic modulation may provide a promising strategy to enhance CRISPR–Cas9‐mediated gene knock‐in.
Xin Chen +9 more
wiley +1 more source
Journal of Fish Diseases, EarlyView.ABSTRACT Crayfish plague, caused by Aphanomyces astaci (Aa), is an infectious disease invasive in Europe, where its rapid spread has resulted in sharp declines of native crayfish species. Monitoring currently relies on a highly sensitive, but costly and time‐consuming qPCR approach.
Christopher Heine +5 more
wiley +1 more source
Photocleavable Guide crRNAs for a Light-Controllable CRISPR/Cas9 System
International Journal of Molecular SciencesThe design of controllable and precise RNA-targeted CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) systems is an important problem of modern molecular biology and genetic technology. Herein, we have designed a series of photocleavable guide CRISPR RNAs (crRNA) and their 2′-modified (2′-fluoro and locked nucleic acid) analogs ...
Lubov Sakovina +6 more
openaire +2 more sources