Results 121 to 130 of about 14,674 (226)

CrRNA sequences used for LDLR/PCSK9 targeting cleavage.

CrRNA sequences used for LDLR/PCSK9 targeting cleavage.
Hiroki Shiomi (718724), Takahiro Horie (337141), Takeshi Kimura (159578), Satoshi Koyama (784927), Koji Hasegawa (337158), Yutaka Suzuki (1379), Masahiro Kimura (2507551), Osamu Baba (634641), Naoya Sowa (634643), Yasuhiro Nakashima (825519), Ayako Suzuki (380214), Sijia Xu (3827086), Koh Ono (337143), Yugo Yamashita (822613)   +13 more
core   +1 more source

CRISPR/Cas9‐mediated Genome‐editing Reveals 10 Testis‐enriched Genes and One Non‐testis‐enriched Gene are Dispensable for Male Fecundity in Mice

Andrology, Volume 14, Issue 7, Page 2150-2159, October 2026.
ABSTRACT Background More than 1000 genes have been identified as predominantly expressed in the human testis. Advances in gene editing technologies have enabled the rapid and efficient generation of genetically engineered mice. This approach facilitates the screening of genes essential for spermatogenesis by analyzing knockout mouse models.
Yumiao Qiu, Keisuke Shimada, Masahito Ikawa   +2 more
wiley   +1 more source

Construction of Erwinia amylovora mutants via crRNA expression

Phytopathology Research
Abstract Genome editing is essential for studying gene function for pathogens such as Erwinia amylovora , which causes devastating fire blight in fruit trees of the family Rosaceae. CRISPR/Cas genome editing technology has been widely used in various model organisms, but
Yu Dong, Yuan Jiang, Chunhe Hu, Lin Liu, Wenwen Li, Liying Yang, Baiming Cui, Yinying Zheng   +7 more
openaire   +1 more source

Estrogen/Esr2b signaling‐dependent, female‐biased expression of a neuropeptide Y gene in the medaka brain

Journal of Neuroendocrinology, Volume 38, Issue 10, October 2026.
Abstract A recent study showed that female medaka lacking the estrogen receptor gene esr2b are unreceptive to male courtship and instead court other females. In this study, we investigated the brain transcriptome of esr2b‐deficient females to explore the underlying mechanisms of this behavioral phenotype, and identified npyb, one of the two ...
Yuki Homan, Yuji Nishiike, Mika Sato‐Tsukamoto, Daisuke Saito, Mikita Suyama, Yasuyuki Ohkawa, Fumiya Takahashi, Takashi Baba, Ken‐ichirou Morohashi, Kataaki Okubo   +9 more
wiley   +1 more source

Iterative Genome Engineering Platform Enables Efficient Sucrose Biosynthesis From CO2 in Photosynthetic Synechococcus elongatus UTEX 2973

Plant Biotechnology Journal, Volume 24, Issue 10, Page 5284-5299, October 2026.
ABSTRACT The single crossover occurring via homologous recombination is a common phenomenon existing among microbes like Escherichia coli, Bacillus subtilis, Vibrio natriegens, Gluconobacter oxydans and most cyanobacteria species, threatening the stability of engineered strains and challenging iterative genetic engineering. Among them, we take the fast‐
Shubin Li, Tao Sun, Dailin Liu, Tong Zhang, Lei Chen, Weiwen Zhang   +5 more
wiley   +1 more source

Mappingin vivogenetic interactomics through Cpf1 crRNA array screening

, 2017
Genetic interactions lay the foundation of biological networks in virtually all organisms. Due to the complexity of mammalian genomes and cellular architectures, unbiased mapping of genetic interactionsin vivois challenging. Cpf1 is a single effector RNA-
Guangchuan Wang, Sidi Chen, Adan Codina, Ryan D. Chow, Lupeng Ye   +4 more
core   +1 more source

SWITCHER, a CRISPR-inducible floxed wild-type Cre regulating CRISPR activity

Communications Biology
Although several Cre-regulated CRISPR/Cas platforms exist, a CRISPR/Cas-controlled Cre-system remains a challenge. Here, we present a genetic switch we term SWITCHER based on a floxed wild-type Cre-construct representing a CRISPR-inducible and self ...
Björn Schuster, Ivana Dobiášovská, Jovana Ćurčić, Petr Pajer, Šimon Borna, Petr Bartůněk   +5 more
doaj   +1 more source

‘Splice-at-will’ Cas12a crRNA engineering enabled direct quantification of ultrashort RNAs

Nucleic Acids Research
Abstract We present a robust ‘splice-at-will’ CRISPR RNA (crRNA) engineering mechanism that overcomes the limitations of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system in directly detecting ultrashort RNAs.
Xinrui Fei, Chao Lei, Wei Ren, Chenghui Liu   +3 more
openaire   +2 more sources

A minimum crRNA guide length of 17 nucleotides is necessary to engage Cas9 target cleavage.

, 2014
A. Sequence of crRNA guides of variable length used in the current study. B. EMSA (left) and cleavage assay (right) of Cas9/tracrRNA/crRNA combinations using p53 [Exon 7] as probe and resolved on a 5% native polyacrylamide gel and 10% polyacrylamide/8M ...
Hisashi Miura (638238), Regina Cencic (39480), Sylvain Ethier (638240), Francis Robert (28516), Josée Dostie (262897), Abba Malina (638239), Jerry Pelletier (28518), T. Martin Schmeing (638241)   +7 more
core   +1 more source

Engineered CRISPR-Cas13a system with enhanced target RNA cleavage activity and reduced collateral activity for therapeutic applications

Molecular Therapy: Nucleic Acids
The CRISPR-Cas13 system exhibits potent RNA cleavage activity and has been widely utilized for RNA-targeting applications. However, its collateral cleavage of bystander RNAs limits in vivo therapeutic applications. In this study, we generated a series of
Wenxia Zhang, Haili Wang, Dongzi Liu, Xi Mao, Yi Zhang, Yimu Yang, Zehui Liu, Tong Pan, Yingle Liu, Qi Zhang   +9 more
doaj   +1 more source