Results 131 to 140 of about 14,674 (226)

Genetic determinants of PAM-dependent DNA targeting and pre-crRNA processing in Sulfolobus islandicus

, 2013
Bacteria and Archaea encode clustered, regularly interspaced, short palindromic repeat (CRISPR) systems to confer adaptive immunity to invasive viruses and plasmids.
Hallstrøm, Søren, Liang, Yun Xiang, She, Qunxin, Peng, Wenfang, Li, Huan, Peng, Nan   +5 more
core   +1 more source

Deep learning enhancing guide RNA design for CRISPR/Cas12a‐based diagnostics

iMeta
Rapid and accurate diagnostic tests are fundamental for improving patient outcomes and combating infectious diseases. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Cas12a‐based detection system has emerged as a promising solution
Baicheng Huang, Ling Guo, Hang Yin, Yue Wu, Zihan Zeng, Sujie Xu, Yufeng Lou, Zhimin Ai, Weiqiang Zhang, Xingchi Kan, Qian Yu, Shimin Du, Chao Li, Lina Wu, Xingxu Huang, Shengqi Wang, Xinjie Wang   +16 more
doaj   +1 more source

EcCas6e-based antisense crRNA for gene repression and RNA editing in microorganisms

Nucleic Acids Research
Abstract Precise gene regulation and programmable RNA editing are vital RNA-level regulatory mechanisms. Gene repression tools grounded in small non-coding RNAs, microRNAs, and CRISPR-dCas proteins, along with RNA editing tools anchored in Adenosine Deaminases acting on RNA (ADARs), have found extensive application in molecular biology ...
Mutong Li, Zhaohui Cai, Shucheng Song, Xinmin Yue, Wenyu Lu, Shuquan Rao, Chuanbo Zhang, Chaoyou Xue   +7 more
openaire   +2 more sources

Efficient Genome Editing Using ‘NanoMEDIC’ AsCas12a-VLPs Produced with Pol II-Transcribed crRNA

Virus-like particles (VLPs) are an attractive vehicle for the delivery of Cas nuclease and guide RNA ribonucleoprotein complexes (RNPs). Most VLPs are produced by packaging SpCas9 and its sgRNA, which is expressed from the RNA polymerase III (Pol III ...
Dmitriy V. Mazurov, Mikhail V. Shepelev, Natalia A. Kruglova, Sofiia E. Borovikova   +3 more
core   +1 more source

Impact of predictive selection of LbCas12a CRISPR RNAs upon on‐ and off‐target editing rates in soybean

Plant Direct
Clustered regularly interspaced short palindromic repeats (CRISPR) technology has revolutionized creating targeted genetic variation in crops. Although CRISPR enzymes have been reported to have high sequence‐specificity, careful design of the editing ...
Linda Rymarquis, Chenxi Wu, Diane Hohorst, Miguel Vega‐Sanchez, Thomas E. Mullen, Vijetha Vemulapalli, Douglas R. Smith   +6 more
doaj   +1 more source

Visualization of endogenous gene expression by knock-in of the eGFP reporter using the crRNA-tracrRNA-Cas9 protein complex.

, 2015
(A) Whole-mount in situ hybridization with an epdr1 probe. (B) An uninjected embryo. (C) The embryo injected two crRNAs (epdr1-crRNA; 25 pg + Mbait-crRNA; 25 pg), tracrRNA (100 pg) and Mbait-hs-eGFP (25 pg) with Cas9 protein (400 pg).
Satoshi Ota (154675), Rie Ohga (744856), Atsuo Kawahara (744857), Kiyohito Taimatsu (744855), Hirohito Kotani (744854)   +4 more
core   +1 more source

Binding Data for Cas9, dmCas9, and crRNA Substrates.

, 2014
aReported as the average and standard error of the mean from 2–3 independent experiments.bCalculated by dividing each Kd value by the Kd for Cas9:cr20:tracrRNA:p53[Exon 7].Binding Data for Cas9, dmCas9, and crRNA Substrates.
Hisashi Miura (638238), Regina Cencic (39480), Sylvain Ethier (638240), Francis Robert (28516), Josée Dostie (262897), Abba Malina (638239), Jerry Pelletier (28518), T. Martin Schmeing (638241)   +7 more
core   +1 more source

Structural basis for target DNA cleavage and guide RNA processing by CRISPR-Casλ2

Communications Biology
RNA-guided CRISPR-Cas nucleases are widely used as versatile genome-engineering tools. Among the diverse CRISPR-Cas effectors, CRISPR-Casλ—also referred to as Cas12n—is a recently identified miniature type V nuclease encoded in phage genomes.
Satoshi N. Omura, Lauren E. Alfonse, Alexa Ornstein, Hayato Morinaga, Hisato Hirano, Yuzuru Itoh, Gabrielle Munoz, Anthony J. Garrity, Gregory R. Hoffman, Tia DiTommaso, Winston X. Yan, David R. Cheng, David A. Scott, Zachary Maben, Osamu Nureki   +14 more
doaj   +1 more source

Identification of a key nucleotide influencing Cas12a crRNA activity for universal photo-controlled CRISPR diagnostics

Nature Communications
Developing a one-pot assay is a critical strategy for enhancing the applicability of CRISPR-based molecular diagnostics; however, it is hindered by CRISPR cleavage interfering with nucleic acid amplification templates. Photo-regulation strategies provide
Tian Tian, Hongrui Xiao, Xinyi Guo, Yuxin Chen, Zhiqiang Qiu, Ting Zhang, Meiyu Chen, Weiwei Qi, Peige Cai, Meng Cheng, Xiaoming Zhou   +10 more
doaj   +1 more source

Harnessing Multiplex crRNA in the CRISPR/Cas12a System Enables an Amplification-Free DNA Diagnostic Platform for ASFV Detection

CRISPR-associated (Cas) protein systems have been increasingly incorporated in nucleic-acid diagnosis. CRISPR/Cas12a can cleave single-stranded DNA (ssDNA) after being guided to the target double-stranded DNA (dsDNA) with crRNA, making it a specific tool
He Meng (33363), Chao Qin (119135), Muchu Zeng (13108914), Lai Yan Li (13108917), Zhuoru Li (8544069), Gaoyuan Sheng (13108920), Yuqing Ke (8809925), Xianting Ding (4985153), Zhiyi Zhuang (13123625)   +8 more
core   +1 more source