Results 31 to 40 of about 14,674 (226)
Microbiology Spectrum, 2022 The clustered regularly interspaced palindromic repeat (CRISPR)-associated (Cas) system functions classically as a prokaryotic defense system against invading mobile genetic elements, such as phages, plasmids, and viruses.
Meng Nie +8 more
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Programmable sequential mutagenesis by inducible Cpf1 crRNA array inversion [PDF]
Nature Communications, 2018 AbstractMutations and genetic alterations are often sequentially acquired in various biological and pathological processes, such as development, evolution, and cancer. Certain phenotypes only manifest with precise temporal sequences of genetic events. While multiple approaches have been developed to model the effects of mutations in tumorigenesis, few ...
Ryan D. Chow, Hyunu Ray Kim, Sidi Chen
openaire +3 more sources
Decision-Making in Cascade Complexes Harboring crRNAs of Altered Length [PDF]
Cell Reports, 2019 The multi-subunit type I CRISPR-Cas surveillance complex Cascade uses its crRNA to recognize dsDNA targets. Recognition involves DNA unwinding and base-pairing between the crRNA spacer region and a complementary DNA strand, resulting in formation of an R-loop structure.
Songailiene, Inga +7 more
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Reprogrammed tracrRNAs enable repurposing of RNAs as crRNAs and sequence-specific RNA biosensors
Nature Communications, 2022 In type II CRISPR systems, the guide RNA (gRNA) comprises a CRISPR RNA (crRNA) and a hybridized trans-acting CRISPR RNA (tracrRNA), both being essential in guided DNA targeting functions. Here the authors investigate the programmability of crRNA-tracrRNA
Yang Liu +9 more
doaj +1 more source
Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity [PDF]
, 2015 EC is supported by the Alexander von Humboldt Foundation, the German Federal Ministry for Education and Research, the Helmholtz Association, the Göran Gustafsson Foundation, the Swedish Research Council, the Kempe Foundation and Umeå University.
White, Malcolm F. +4 more
core +1 more source
Electrostatic surface potential of the Lb2Cas12a, apo, crRNA bound, crRNA DNA bound complex.
, 2023 (A) Electrostatic surface potential of the apo Lb2Cas12a (Crystal structure). (B) Electrostatic surface potential of the Lb2Cas12a-crRNA complex (Crystal structure).
Sun Meng (11007860) +8 more
core +1 more source
Biomolecules, 2023 The CRISPR-Cas system has been widely used for genome editing due to its convenience, simplicity and flexibility. Using a plasmid-carrying Cas protein and crRNA or sgRNA expression cassettes is an efficient strategy in the CRISPR-Cas genome editing ...
Bo-Chou Chen, Yu-Zhen Chen, Huan-Yu Lin
doaj +1 more source
Cas6 specificity and CRISPR RNA loading in a complex CRISPR-Cas system [PDF]
, 2014 This research was funded in part by Biotechnology and Biological Sciences Research Council [BB/K000314/1]. The APC was paid through RCUK OA block grant funds.CRISPR-Cas is an adaptive prokaryotic immune system, providing protection against viruses and ...
Malcolm F. White +5 more
core +1 more source
, 2023 (A) A red rectangle marks the area of Cas10-Csm which is magnified to the right. The detailed interactions of S. epidermidis Csm3 with crRNA are shown.
Asma Hatoum-Aslan (4451431) +7 more
core +1 more source
Quantitative and modularized CRISPR/dCas9-dCpf1 dual function system in Saccharomyces cerevisiae
Frontiers in Bioengineering and Biotechnology, 2023 Introduction: Both CRISPR/dCas9 and CRISPR/dCpf1 genome editing systems have shown exciting promises in modulating yeast cell metabolic pathways. However, each system has its deficiencies to overcome.
Qing Feng +4 more
doaj +1 more source