Results 21 to 30 of about 14,674 (226)

CRISPRTarget [PDF]

RNA Biology, 2013
The bacterial and archaeal CRISPR/Cas adaptive immune system targets specific protospacer nucleotide sequences in invading organisms. This requires base pairing between processed CRISPR RNA and the target protospacer. For type I and II CRISPR/Cas systems, protospacer adjacent motifs (PAM) are essential for target recognition, and for type III ...
Biswas, A., Gagnon, J.N., Brouns, S.J.J., Fineran, P.C., Brown, C.M.   +4 more
openaire   +2 more sources

crRNA-binding pocket in Lb2Cas12a-crRNA complex.

, 2023
(A) Interactions between REC lobe and NUC lobe in Lb2Cas12a-crRNA complex. Left: Crystal structure of Lb2Cas12a-crRNA in tube representation. Right: The close-up view of interaction between WED, RuvC domain, and REC lobe.
Sun Meng (11007860), Shi Jian (14790680), Yuren Adam Yuan (14790683), Chacko Jobichen (43238), Satoru Machida (14790671), J. Sivaraman (31440), Li Jianwei (14790668), Randy J. Read (14790674), Chen Hongying (14790677)   +8 more
core   +1 more source

Cryo-EM structures of Lb2Cas12a-crRNA-DNA complex.

, 2023
(A) Overall structure of the Lb2Cas12a-crRNA-DNA, at 3.95 Å resolution. These figures are prepared by Chimera program; different domains are distinguished by color, surface transparency: 70%. (B) crRNA-DNA duplex in Cryo-EM density map.
Sun Meng (11007860), Shi Jian (14790680), Yuren Adam Yuan (14790683), Chacko Jobichen (43238), Satoru Machida (14790671), J. Sivaraman (31440), Li Jianwei (14790668), Randy J. Read (14790674), Chen Hongying (14790677)   +8 more
core   +1 more source

Foldback-crRNA-Enhanced CRISPR/Cas13a System (FCECas13a) Enables Direct Detection of Ultrashort sncRNA

, 2023
The CRISPR/Cas13a system has promising applications in clinical small noncoding RNA (sncRNA) detection because it is free from the interference of genomic DNA.
Qianling Zhang, Xinyao Yang (1482325), Siyuan Luo, Yizhen Liu (1970200), Xueji Zhang, Xinyao Yang, Xueji Zhang (392238), Conghui Liu, Yong Chen (109188), Siyuan Luo (4490908), Conghui Liu (542714), Yizhen Liu, Yibin Zhang (1426579), Qianling Zhang (5877506), Yong Chen, Yibin Zhang   +15 more
core   +1 more source

crRNA-dependent double-stranded DNA cleavage by Lb2Cas12a.

, 2023
(A) dsDNA substrate cleavage by Lb2Cas12a with crRNA, TS: Target dsDNA; C: Control, dsDNA only; S: Substrate dsDNA; P: Cleaved products. (B) Cleavage site of target DNA analyzed by Sanger sequencing, black line: Canonical base pairs; gray line ...
Sun Meng (11007860), Shi Jian (14790680), Yuren Adam Yuan (14790683), Chacko Jobichen (43238), Satoru Machida (14790671), J. Sivaraman (31440), Li Jianwei (14790668), Randy J. Read (14790674), Chen Hongying (14790677)   +8 more
core   +1 more source

Structure and mechanism of the RNA dependent RNase Cas13a from Rhodobacter capsulatus

Communications Biology, 2022
The structure of the Cas13a RNase from the purple bacterium R. capsulatus in complex with its crRNA is reported here. The study reveals that the enzyme combines shape and sequence-specific readout to recognize its crRNA.
Leonhard M. Kick, Marie-Kristin von Wrisberg, Leander S. Runtsch, Sabine Schneider   +3 more
doaj   +1 more source

CRISPR guides induce gene silencing in plants in the absence of Cas

Genome Biology, 2022
Background RNA-targeting CRISPR-Cas can provide potential advantages over DNA editing, such as avoiding pleiotropic effects of genome editing, providing precise spatiotemporal regulation, and expanded function including antiviral immunity.
Veerendra Kumar Sharma, Sandeep Marla, Wenguang Zheng, Divya Mishra, Jun Huang, Wei Zhang, Geoffrey Preston Morris, David Edward Cook   +7 more
doaj   +1 more source

CRISPR interference : a structural perspective [PDF]

, 2013
This article was made open access through BIS OA funding. The laboratory is funded by grants from the Biotechnology and Biological Sciences Research Council (BBSRC).CRISPR (cluster of regularly interspaced palindromic repeats) is a prokaryotic adaptive ...
Naismith, Jim, White, Malcolm F, Reeks, Judith Anne   +2 more
core   +1 more source

Enhanced FnCas12a-Mediated Targeted Mutagenesis Using crRNA With Altered Target Length in Rice

Frontiers in Genome Editing, 2020
The CRISPR/Cas12a (Cpf1) system utilizes a thymidine-rich protospacer adjacent motif (PAM) and generates DNA ends with a 5′ overhang. These properties differ from those of CRISPR/Cas9, making Cas12a an attractive alternative in the CRISPR toolbox ...
Katsuya Negishi, Masafumi Mikami, Masafumi Mikami, Seiichi Toki, Seiichi Toki, Seiichi Toki, Masaki Endo, Masaki Endo   +7 more
doaj   +1 more source

Editing efficiencies of different crRNA expression strategies.

, 2022
(A) The two target sequences of GL1-M and GL1-N. (B) Schematic diagram of T-DNA structure of different crRNA expression strategies. RB and LB, T-DNA right and left borders, re-spectively. U6p, Arabidopsis U6 gene promoter.
Yan Zhang (8098), Qiang Zhang (45005), Yiping Chai (12295154)   +2 more
core   +1 more source