Results 31 to 40 of about 83,279 (184)
Integrating AI and CRISPR Cas13a for rapid detection of tomato brown rugose fruit virus [PDF]
Scientific ReportsThe Tomato Brown Rugose Fruit Virus (ToBRFV) has recently emerged as a serious threat to global tomato production, underscoring the need for rapid and sensitive diagnostic tools.
Marzieh Karimi +4 more
doaj +2 more sources
CRISPR-Cas13a-powered electrochemical biosensors for RNA-based disease diagnostic and monitoring
Sensors and Actuators ReportsNucleic acids serve as specific, selective, and sensitive components in molecular diagnostics, offering efficient and high-precision results. Unlike DNA, RNA expression reflects real-time cellular activity, allowing for the monitoring of disease ...
Salma Nur Zakiyyah +9 more
doaj +2 more sources
Development of CRISPR/Cas13a-based assays for the diagnosis of Schistosomiasis
eBioMedicine, 2023 Schistosomiasis is a disease that significantly impacts human health in the developing world. Effective diagnostics are urgently needed for improved control of this disease. CRISPR-based technology has rapidly accelerated the development of a revolutionary and powerful diagnostics platform, resulting in the advancement of a class of ultrasensitive ...
Skye R. MacGregor +14 more
openaire +5 more sources
Rapid Detection of Tomato Spotted Wilt Virus With Cas13a in Tomato and Frankliniella occidentalis
Frontiers in Microbiology, 2021 As one of the top 10 plant viruses, the severity of losses to crop productivity caused by the tomato spotted wilt virus (TSWV) has resulted in an urgent need to develop a more sensitive and rapid method of detection.
Wanhong Zhang +9 more
doaj +1 more source
Structural studies of CRISPR-associated proteins [PDF]
, 2013 Clustered regularly interspaced short palindromic repeats (CRISPRs) act to prevent viral infection and horizontal gene transfer in prokaryotes. The genomic CRISPR array contains short sequences (“spacers”) that are derived from foreign genetic elements ...
Reeks, Judith
core +2 more sources
, 2022 The disease caused by severe acute respiratory syndrome coronavirus, SARS-CoV-2, is termed COVID-19. Even though COVID-19 has been out for more than two years, it is still causing a global pandemic.
Zhanpeng Ren (12077826) +9 more
core +1 more source
CRISPR-Cas13a Cleavage of Dengue Virus NS3 Gene Efficiently Inhibits Viral Replication
Molecular Therapy: Nucleic Acids, 2020 The CRISPR-Cas9 system has been applied to DNA editing with precision in eukaryotic and prokaryotic systems, but it is unable to edit RNA directly. A recently developed CRISPR-Cas13a system has been shown to be capable of effectively knocking down RNA ...
Hao Li +10 more
doaj +1 more source
Zhongguo shipin weisheng zazhi, 2023 ObjectiveTo establish a rapid， sensitive and specific detection method for 4 diarrheal bacteria （Salmonella， Shigella， Vibrio cholera and Escherichia coli O157：H7） by the Clustered regularly interspaced short palindromic repeats associated protein 13a ...
AN Bailin +6 more
doaj +1 more source
CRISPR-Cas13a-Based Detection for Bovine Viral Diarrhea Virus [PDF]
Frontiers in Veterinary Science, 2021 Bovine Viral Diarrhea Virus (BVDV) is the main pathogen of bovine viral diarrhea disease (BVD), which leads to enormous economic losses in the cattle industry. A sensitive and specific detection for BVDV is advantageous to the control of BVDV. Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have been used for detecting ...
Rui Yao +16 more
openaire +3 more sources
CRISPR-Cas13a diagnostics for circulating microbial transcripts in pancreatic cancer. [PDF]
Ann Med Surg (Lond)Pancreatic ductal adenocarcinoma (PDAC) is the seventh leading cause of global cancer deaths, with a 5-year survival rate below 11% and over 80% of cases diagnosed at advanced, unresectable stages. Current biomarkers such as CA19-9 show suboptimal diagnostic accuracy (sensitivity 65–75%; specificity 70–80%), necessitating more ...
Mehmood MS, Iqbal I, Hajj F.
europepmc +3 more sources