Results 61 to 70 of about 83,279 (184)

Transport–Reaction–Signal Coupling in Lateral Flow Assays for Next‐Generation Point‐of‐Care Diagnostics

Advanced Science, EarlyView.
Lateral flow assays are evolving into interdisciplinary, system‐level sensing platforms. Recent innovations are deconstructed through coupled phases of mass transfer, reaction, and signal transduction, revealing interdependence across porous materials, interfacial chemistry, nanolabels, readout devices, and data‐enabled analysis.
Yan Pan, Yuewei Li, Chao Mi, Zhengwen Yang, Lana McClements, Steven J. Langford, Jiayan Liao   +6 more
wiley   +1 more source

Rapid Detection of blaKPC in Carbapenem-Resistant Enterobacterales Based on CRISPR/Cas13a

Current Microbiology, 2023
AbstractKlebsiella pneumoniae carbapenemase (KPC) is a crucial enzyme that causes carbapenem resistance in Enterobacterales, and infections by these "superbugs" are extremely challenging to treat. Therefore, there is a pressing need for a rapid and accurate KPC detection test to control the prevalence of carbapenem-resistant Enterobacterales (CREs). In
Mingjun Liang, Bin Xiao, Lidan Chen, Xiaoyan Huang, Jinchao Li, Zhenzhan Kuang, Xinping Chen, Xiuna Huang, Zhaohui Sun, Linhai Li   +9 more
openaire   +2 more sources

CRISPR-mediated targeted mRNA degradation in the archaeon Sulfolobus solfataricus [PDF]

, 2014
European SulfoSYS-project [SysMo P–N-01-09-23] and grant [9P23000 and P25369] by the Austrian Research fund (to C.S.) and by grant [BB/K000314/1] from the Biotechnology and Biological Sciences Research Council (to M.F.W.). Funding for open access charge:
Manica, A., Zebec, Z., Schleper, C., Zhang, J., White, M.F.   +4 more
core   +1 more source

CRISPR/Cas13a-Assisted accurate and portable hepatitis D virus RNA detection

Emerging Microbes and Infections, 2023
Background & Aims Hepatitis delta virus (HDV) infection accelerates the progression of chronic hepatitis B virus (HBV) infection, posing a large economic and health burden to patients.
Yuan Tian, Zihao Fan, Xiangying Zhang, Ling Xu, Yaling Cao, Zhenzhen Pan, Yinkang Mo, Yao Gao, Sujun Zheng, Jing Huang, Huaibin Zou, Zhongping Duan, Hao Li, Feng Ren   +13 more
doaj   +1 more source

Revolutionizing Healthcare With Paper‐Based Nucleic Acid Testing

Exploration, EarlyView.
This work summarizes strategies to enhance paper‐based devices for nucleic acid testing. Key approaches include optimizing paper platforms, improving nucleic acid amplification, and refining labeling/signaling methods. These advancements aim to boost sensitivity, speed, and usability, making paper‐based diagnostics more effective for point‐of‐care ...
Hong Zhang, Yi Yang, Heng Li, Xiaolin Hu, Hui Xiao, Dou Wang, Sergio Benardini, Wei Gu, Yang Luo   +8 more
wiley   +1 more source

Visual Detection of Duck Tembusu Virus With CRISPR/Cas13: A Sensitive and Specific Point-of-Care Detection

Frontiers in Cellular and Infection Microbiology, 2022
Duck tembusu virus (DTMUV), which causes huge economic losses for the poultry industries in Southeast Asia and China, was first identified in 2010. DTMUV disease has become an important disease that endangers the duck industry. A sensitive, accurate, and
Dongdong Yin, Lei Yin, Jieru Wang, Xuehuai Shen, Xiaocheng Pan, Hongyan Hou, Ruihong Zhao, Xiaomiao Hu, Guijun Wang, Kezong Qi, Yin Dai   +10 more
doaj   +1 more source

Engineered molecular diagnostic strategies for hepatocellular carcinoma: Integrating flexible photonic barcodes, liquid biopsies, and AI‐enhanced sensing platforms

FlexMat, EarlyView.
Abstract Early diagnosis of hepatocellular carcinoma (HCC) remains challenging because conventional serum biomarkers, especially alpha‐fetoprotein (AFP), show limited sensitivity for early‐stage disease. Recent progress in engineered molecular diagnostics provides new opportunities to improve HCC detection through integrated material, molecular, and ...
Jingzhi Xue, Wanqi Yang, Xiaoyu Liu, Xuehui Chu, Wei Li, Jinglin Wang   +5 more
wiley   +1 more source

Guide-bound structures of an RNA-targeting A-cleaving CRISPR–Cas13a enzyme [PDF]

Nature Structural & Molecular Biology, 2017
CRISPR adaptive immune systems protect bacteria from infections by deploying CRISPR RNA (crRNA)-guided enzymes to recognize and cut foreign nucleic acids. Type VI-A CRISPR-Cas systems include the Cas13a enzyme, an RNA-activated RNase capable of crRNA processing and single-stranded RNA degradation upon target-transcript binding.
Gavin J Knott, Alexandra East-Seletsky, Joshua C Cofsky, James M Holton, Emeric Charles, Mitchell R O'Connell, Jennifer A Doudna   +6 more
openaire   +3 more sources

Ordered assembly of Cas12a for better performance of CRISPR/Cas system with application to the diagnosis of dental caries

Interdisciplinary Medicine, EarlyView.
Here, we present a tetrahedral DNA framework (TDF)‐mediated Cas12a ordered assembly strategy. TDFs arrange CRISPR/Cas12a into ordered structures controllably, enhancing catalytic efficiency through localized Cas12a hotspots while maintaining spatial intervals to facilitate substrate diffusion.
Yining Zhao, Panpan Qiao, Yujing Zeng, Murtaza Sayed, Qin Mi, Juan Zhang, Genxi Li   +6 more
wiley   +1 more source

CRISPR/Cas13a-assisted AMP generation for SARS-CoV-2 RNA detection using a personal glucose meter

Biosensors and Bioelectronics: X, 2022
Herein, we described a washing- and label-free clustered regularly interspaced short palindromic repeats (CRISPR)/LwaCas13a-based RNA detection method utilizing a personal glucose meter (PGM), which relies on the trans-cleavage activity of CRISPR/Cas13a ...
Junhyun Park, Hyogu Han, Jae Hoon Jeung, Hyowon Jang, Chihyun Park, Jun Ki Ahn   +5 more
doaj   +1 more source