Recombinase-Aided Amplification Coupled with Lateral Flow Dipstick for Efficient and Accurate Detection of Porcine Parvovirus [PDF]
Life, 2021 Porcine parvovirus (PPV) infection is the primary cause of SMEDI (stillbirth; mummification; embryonic death; infertility) syndrome, which is a global burden for the swine industry.
Yihong He +6 more
doaj +4 more sources
A Review of the Application of Recombinase Polymerase Amplification, Recombinase-Aided Amplification and Enzymatic Recombinase Amplification in Rapid Detection of Foodborne Pathogens [PDF]
Shipin Kexue, 2023 With the improvement of people’s living standards, food safety has attracted more and more social attention. Organisms, especially microorganisms, are the most important factor that affects food safety.
WANG Shuai, YANG Yange, WU Zhanwen, LI Hongna, LI Tao, SUN Dongmei, YUAN Fei
doaj +2 more sources
Enhancement of a recombinase-aided amplification assay using betaine and pullulan
Infectious Medicine, 2022 Background: Nucleic acid amplification enhancers suitable for use in a recombinase-aided amplification (RAA) assay were studied for the first time, and amplification of a long-fragment (509 bp) was initially explored.
Jinrong Wang +8 more
doaj +3 more sources
Use of a rapid recombinase-aided amplification assay for Mycoplasma pneumoniae detection [PDF]
BMC Infectious Diseases, 2020 Background Mycoplasma pneumoniae is one of the most common causative pathogens of community-acquired pneumonia (CAP), accounting for as many as 30–50% of CAP during peak years.
Guanhua Xue +7 more
doaj +3 more sources
Reverse transcription recombinase-aided amplification assay for H5 subtype avian influenza virus [PDF]
Virology Journal, 2022 Background The H5 subtype avian influenza virus (AIV) has caused huge economic losses to the poultry industry and is a threat to human health. A rapid and simple test is needed to confirm infection in suspected cases during disease outbreaks.
Suchun Wang +13 more
doaj +6 more sources
Development of a recombinase-aided amplification assay for rapid detection of human norovirus GII.4 [PDF]
BMC Infectious Diseases, 2021 Background Human noroviruses are one of the main causes of foodborne illnesses and represent a serious public health concern. Rapid and sensitive assays for human norovirus detection are undoubtedly necessary for clinical diagnosis, especially in regions
Zhiwei Qin +10 more
doaj +3 more sources
Development of a reverse transcription recombinase-aided amplification assay for detection of Getah virus [PDF]
Scientific Reports, 2021 GETV, an arbo-borne zoonotic virus of the genus Alphavirus, which causes diarrhea and reproduction disorders in swine, lead to serious economic losses to the swine industry in China.
Mincai Nie +6 more
doaj +3 more sources
Diagnostics, 2022 Varicella-zoster virus (VZV) is the etiological agent of varicella (chickenpox) and herpes zoster (shingles). VZV infections are ubiquitous and highly contagious, and diagnosis is mostly based on the assessment of signs and symptoms.
Nicolas Berthet +14 more
core +4 more sources
BMC Infectious Diseases, 2019 Background Hepatitis B virus (HBV) infection is the major public health problem worldwide. In clinical practice, serological and molecular assays are the most commonly used diagnostic methods to detect HBV infection in clinical practices. Methods Here we
Xin-xin Shen +10 more
doaj +2 more sources
Dye‐based recombinase‐aided amplification assay with enhanced sensitivity and specificity
iLABMEDBackground Fluorescent recombinase‐aided amplification (RAA) assays are increasingly being used in the detection of a variety of pathogens and have the advantages of rapidity and simplicity and similar sensitivity and specificity, compared with real‐time
Zijin Zhao +5 more
doaj +2 more sources