Results 21 to 30 of about 520,105 (221)
Viruses, 2022 Porcine epidemic diarrhea virus (PEDV) is a coronavirus currently widespread worldwide in the swine industry. Since PEDV was discovered in China in 1984, it has caused huge economic losses in the swine industry.
Qiqi Zhao +7 more
core +4 more sources
Frontiers in Cellular and Infection Microbiology, 2022 BackgroundMalaria is a global public health problem. China has had no case of indigenous malaria since 2016. However, imported cases of malaria remain an issue among travelers, overseas workers, and foreign traders.
Hong Lin +6 more
doaj +3 more sources
Research Note: Real-time fluorescence-based recombinase-aided amplification for rapid detection of Mycoplasma synoviae [PDF]
Poultry Science: Mycoplasma synoviae (MS) is an essential pathogenic mycoplasma in poultry worldwide, posing a serious threat to the poultry industry's health. Timely detection is imperative for early diagnosis, prevention, and control of MS infection.
Wenlong Xia +9 more
doaj +2 more sources
Frontiers in Microbiology, 2022 Chlamydia psittaci is the causative agent of psittacosis, a worldwide zoonotic disease. A rapid, specific, and sensitive diagnostic assay would be benefit for C. psittaci infection control.
Jun Jiao +7 more
doaj +3 more sources
A reverse transcription recombinase-aided amplification assay for the rapid detection of goose astrovirus. [PDF]
Vet Res ForumChina's burgeoning animal husbandry sector has witnessed a notable expansion in goose farming. Among the various health challenges, a novel goose astrovirus (GoAstV) has emerged as a significant threat to the industry, necessitating prompt detection strategies to mitigate its economic impact.
Zheng L, Zhang H.
europepmc +3 more sources
A Fluorescent Recombinase Aided Amplification Assay for Detection of Babesia microti
The Korean Journal of Parasitology, 2022 <i>Babesia microti</i> is one of the most common causative agents of babesiosis. A sensitive and rapid detection is necessary for screening potentially infected individuals. In this study, <i>B. microti</i> cytochrome <i>c</i> oxidase subunit I (<i>cox</i>1) was selected as the target gene, multiple ...
Hong Lin +5 more
openaire +2 more sources
RAP: A Novel Approach to the Rapid and Highly Sensitive Detection of Respiratory Viruses
Frontiers in Bioengineering and Biotechnology, 2021 Recombinase aided amplification (RAA) is an emerging isothermal amplification method used for detecting various pathogens. However, RAA requires a complex and long probe to ensure high sensitivity during fluorescence assay.
Guohao Fan +7 more
doaj +1 more source
Enhancement of Recombinase-Aided Amplification (Raa) Assay by Betaine and Pullulan [PDF]
, 2021 Abstract In this research, nucleic acid amplification enhancers suitable for recombinase-aided amplification (RAA) assay were studied for the first time, and amplification of a long-fragment (509 bp) by the RAA assay was initially explored. Using recombinant plasmids and clinical samples, RAA fluorescence and basic methods were used to evaluate
Jinrong Wang +9 more
openaire +1 more source
Poultry Science, 2020 In this study, specific primers and fluorescent probes were designed to target the thymidine kinase (TK) gene sequence of avian infectious laryngotracheitis virus (ILTV).
Wenjing Wang +7 more
doaj +1 more source
Rapid Visual Detection of <i>Glaesserella parasuis</i> with a Real-Time Recombinase-Aided Amplification Assay. [PDF]
Transbound Emerg Dis, 2023 Glaesserella parasuis is a specific bacterial pathogen of Glässer’s disease which causes significant economic losses to the swine industry. Dependable and rapid detection of G. parasuis is crucial to prevent and control Glässer’s disease outbreaks. In this study, a recombinase-aided amplification (RAA) assay based on the infB gene was developed to ...
Kang H +10 more
europepmc +3 more sources