Results 1 to 10 of about 528,821 (173)

Development and performance of recombinase-aided amplification (RAA) assay for detecting Schistosoma haematobium DNA in urine samples [PDF]

Heliyon, 2023
Rapid diagnosis of urogenital schistosomiasis caused by Schistosoma haematobium requires an accurate and timely assay, especially for low-intensity S. haematobium infection cases and in non-endemic areas. The mitochondrial cytochrome c oxidase 1 (cox1) gene fragment of S. haematobium was selected as detection target as this short fragment, which can be
Kun Yang, Saleh Juma
exaly   +6 more sources

RAA-Cas12a-Tg: A Nucleic Acid Detection System for Toxoplasma gondii Based on CRISPR-Cas12a Combined with Recombinase-Aided Amplification (RAA) [PDF]

Microorganisms, 2021
Toxoplasmosis, caused by the intracellular protozoon Toxoplasma gondii, is a significant parasitic zoonosis with a world-wide distribution. As a main transmission route, human infection can be acquired by the ingestion of T. gondii oocysts from the environment (e.g., soil, water, fruits and vegetables). Regarding the detection of T.
Muhammad Ehsan, Laibao Zheng, Xing-Quan Zhu   +2 more
exaly   +6 more sources

Optimization and Validation of Reverse Transcription Recombinase-Aided Amplification (RT-RAA) for Sorghum Mosaic Virus Detection in Sugarcane

Pathogens, 2023
Sorghum mosaic virus (SrMV) causes sugarcane mosaic disease and has significant adverse economic impacts on the cultivation of sugarcane. This study aimed to develop a rapid isotherm nucleic acid amplification method for detecting SrMV.
Fenglin Wang, Qinmin Liang, Rongman Lv, Shakeel Ahmad, Mishal Bano, Guangzhen Weng, Ronghui Wen   +6 more
doaj   +6 more sources

Light‐Activated Nucleic Acid Amplification Systems Using Photo‐Caged DNA Polymerase or Primers [PDF]

Advanced Genetics
Precise control over nucleic acid amplification is essential for achieving reliable, quantitative, high‐throughput, and multiplexed molecular diagnostics, particularly in point‐of‐care and field settings.
Yang Wu, Meng Wu, Yukun Tian, Hao Chen, Xizhe Sun, Chukang Ma, Jiayao Liu, Fuyu Xia, Yu Liu, Xiaomeng Pei, Jing Wang, Guogang Zhao, Nan Wang, Fangfang Wang, Qing Liu, Fei Yu, Xiaofei Fan, Zehe Wang, Elena Ermilova, Jon S. West, Qi Cheng   +20 more
doaj   +3 more sources

Advances in the application of recombinase-aided amplification combined with CRISPR-Cas technology in quick detection of pathogenic microbes

Frontiers in Bioengineering and Biotechnology, 2023
The rapid diagnosis of pathogenic infections plays a vital role in disease prevention, control, and public health safety. Recombinase-aided amplification (RAA) is an innovative isothermal nucleic acid amplification technology capable of fast DNA or RNA ...
Xiaoping Li, Xiaoping Li, Shuying Zhu, Xinling Zhang, Yanli Ren, Jing He, Jiawei Zhou, Liliang Yin, Gang Wang, Tian Zhong, Ling Wang, Ying Xiao, Ying Xiao, Chunying Zhu, Chengliang Yin, Xi Yu, Xi Yu   +16 more
doaj   +3 more sources

Establishment of reverse transcription recombinase–aided amplification-lateral-flow dipstick and real-time fluorescence–based reverse transcription recombinase–aided amplification methods for detection of the Newcastle disease virus in chickens

Poultry Science, 2020
Newcastle disease is an acute and highly contagious disease of poultry caused by Newcastle disease virus infection, which does great harm to the poultry industry all over the world.
Wenjing Wang, Chunguang Wang, Yun Bai, Peng Zhang, Shanshan Yao, Jingru Liu, Tie Zhang   +6 more
doaj   +3 more sources

Development of nucleic acid testing technology for quarantine pest Globodera rostochiensis based on a species-specific DNA fragment [PDF]

PeerJ
Background Globodera rostochiensis, a potato cyst nematode (PCN) species, is distributed in potato-growing regions across Europe, Asia, Africa, Oceania, and the Americas.
Fengqi Lan, Honghao Li, Zhenji Yin, Yajie Wang, Xiaofeng Liu, Jiahui Yang, Yue Sun, Yingqing Tang, Bingjie Wang, Shiyang Yin, Bing Li, Xiang Tao   +11 more
doaj   +3 more sources

Enhancement of a recombinase-aided amplification assay using betaine and pullulan

Infectious Medicine, 2022
Background: Nucleic acid amplification enhancers suitable for use in a recombinase-aided amplification (RAA) assay were studied for the first time, and amplification of a long-fragment (509 bp) was initially explored.
Jinrong Wang, Guowei Song, Yue Ming, Jing Pan, Ruiqing Zhang, Guohao Fan, Xinxin Shen, Xuejun Ma, Lixin Li   +8 more
doaj   +3 more sources

Development of field-applicable endogenous internally controlled recombinase-aided amplification (EIC-RAA) assays for the detection of human papillomavirus genotypes 6 and 11 using sample releasing agent

Heliyon, 2022
Human papillomavirus (HPV) 6 and 11 are the two most common low-risk HPV subtypes, accounting for more than 90% of condyloma acuminatum. A simple, accurate and rapid screening method to be applied in community-level hospitals is in high demand.Endogenous internally controlled recombinase-assisted amplification (EIC-RAA) assays for HPV6 and 11 were ...
Anna He, Cheng Fang, Yue Ming, He Tan, Mengyi Zhang, Ruiqing Zhang, Jingyi Li, Mingzhu Nie, Fengyu Li, Yaxin Hu, Xinxin Shen, Xiuge Rong, Xuejun Ma   +12 more
exaly   +4 more sources

A novel rapid visual detection assay for Toxoplasma gondii combining recombinase-aided amplification and lateral flow dipstick coupled with CRISPR-Cas13a fluorescence (RAA-Cas13a-LFD)

Parasite, 2022
Toxoplasmosis, a parasitic disease resulting from Toxoplasma gondii infection, remains prevalent worldwide, and causes great harm to immunodepressed patients, pregnant women and newborns. Although various molecular approaches to detect T.
Zhao Jinhong, Li Yuanyuan, Xue Qiqi, Zhu Zhiwei, Zou Minghui, Fang Fang   +5 more
doaj   +3 more sources