Results 21 to 30 of about 528,821 (173)

Sex Identification of a Multispecies Carinatae Birds by Chicken EE0.6 Gene Using Real‐Time Recombinase‐Aid Amplification Assay

Ecology and Evolution
The difficulty in bird sex identification has made molecular sexing an important way to solve this problem. The conventional polymerase chain reaction (PCR) methods are time‐consuming and dependent on laboratory equipment. Recombinase‐aided amplification
Fanwen Zeng, Wanhuan Zhong, Tanzipeng Chen, Guoqian Wang, Jiaqi Sa, Shouquan Zhang, Hengxi Wei, Xuanjiao Chen   +7 more
doaj   +2 more sources

A CRISPR-Cas12a and Quantum-Dot Lateral-Flow Assay for Rapid Species-Level Detection of Trichophyton rubrum. [PDF]

Mycoses
ABSTRACT Background Accurate species‐level diagnosis of dermatophytes, particularly Trichophyton rubrum, is important for guiding targeted antifungal therapy and improving the management of recurrent or atypical superficial mycoses. However, routine microscopy has only moderate sensitivity and cannot resolve fungal species; fungal culture is time ...
Hu X, Xu P, Shen M, Chai X, Tan J, Sun X, Yang L, Wang Q, Tan F.   +8 more
europepmc   +2 more sources

A Rapid, Field-Deployable Diagnostic Platform for Getah Virus Based on RT-RAA and CRISPR EsCas13d. [PDF]

Microb Biotechnol
This study developed a rapid, one‐step, visual detection platform termed ROSVD by integrating RT‐RAA with CRISPR–EsCas13d, enabling simplified sample preparation, high‐sensitivity, on‐site detection of GETV within 30 min without the need for sophisticated equipment.
Baoling L, Lina S, Tong X, Qinrui J, Ling Z, Zhiwen X.   +5 more
europepmc   +2 more sources

Enhancement of Recombinase-Aided Amplification (Raa) Assay by Betaine and Pullulan [PDF]

, 2021
Abstract In this research, nucleic acid amplification enhancers suitable for recombinase-aided amplification (RAA) assay were studied for the first time, and amplification of a long-fragment (509 bp) by the RAA assay was initially explored. Using recombinant plasmids and clinical samples, RAA fluorescence and basic methods were used to evaluate
Jinrong Wang, Guowei Song, Yue Ming, Jing Pan, Ruiqing Zhang, Guohao Fan, Yuan Gao, Xinxin Shen, Xuejun Ma, Lixin Li   +9 more
openaire   +1 more source

A Review of the Application of Recombinase Polymerase Amplification, Recombinase-Aided Amplification and Enzymatic Recombinase Amplification in Rapid Detection of Foodborne Pathogens [PDF]

Shipin Kexue, 2023
With the improvement of people’s living standards, food safety has attracted more and more social attention. Organisms, especially microorganisms, are the most important factor that affects food safety.
WANG Shuai, YANG Yange, WU Zhanwen, LI Hongna, LI Tao, SUN Dongmei, YUAN Fei
doaj   +1 more source

Establishment of two assays based on reverse transcription recombinase-aided amplification technology for rapid detection of H5 subtype avian influenza virus

Microbiology Spectrum, 2023
The avian influenza virus (AIV), a member of the type A influenza virus group, is harbored by avian hosts. The highly pathogenic H5 subtype of AIV has inflicted significant financial damage on the poultry industry and also presents a possible pandemic ...
Yang Li, Jiajing Shang, Yixin Wang, Juan Luo, Wenming Jiang, Xin Yin, Fuyou Zhang, Chunran Deng, Xiaohui Yu, HuaLei Liu   +9 more
doaj   +1 more source

RAP: A Novel Approach to the Rapid and Highly Sensitive Detection of Respiratory Viruses

Frontiers in Bioengineering and Biotechnology, 2021
Recombinase aided amplification (RAA) is an emerging isothermal amplification method used for detecting various pathogens. However, RAA requires a complex and long probe to ensure high sensitivity during fluorescence assay.
Guohao Fan, Ruiqing Zhang, Xiaozhou He, Fengyu Tian, Mingzhu Nie, Xinxin Shen, Xuejun Ma, Xuejun Ma   +7 more
doaj   +1 more source

Quick detection of Carassius auratus herpesvirus (CaHV) by recombinase-aid amplification lateral flow dipstick (RAA-LFD) method

Frontiers in Cellular and Infection Microbiology, 2022
Crucian carp (Carassius auratus) is one of the major freshwater species and is also a common food fish in China. Recently, Carassius auratus herpesvirus (CaHV) could induce fatal viral disease with high mortality of crucian carp, which had caused huge economic losses.
Gui Liu, Yun Zhao, Dan Xu, Xinyu Li, Jie Luo, Wenzong Zhou, Mingyou Li   +6 more
openaire   +3 more sources

Sensitive and specific exonuclease III-assisted recombinase-aided amplification colorimetric assay for rapid detection of nucleic acids

, 2023
The development of a contamination-free and on-site nucleic acid detection platform with high sensitivity and specificity but low-cost for the detection of pathogenic nucleic acids is critical for infectious disease diagnosis and surveillance.
Tao, D., Zhao, S., Steinaa, Lucilla, Sun, H., Ruan, J., Li, X., Han Jianlin, Ren, R., Hemmink, Johanneke D., Xu, B., Zhao, C., Han, X., Xie, S., Xu, J., Zhou, Y., Zhang, J.   +15 more
core   +3 more sources

Development of a recombinase-aided amplification assay for rapid detection of human norovirus GII.4

BMC Infectious Diseases, 2021
Background Human noroviruses are one of the main causes of foodborne illnesses and represent a serious public health concern. Rapid and sensitive assays for human norovirus detection are undoubtedly necessary for clinical diagnosis, especially in regions
Zhiwei Qin, Liang Xue, Weicheng Cai, Junshan Gao, Yueting Jiang, Jiale Yang, Yanhui Liang, Linping Wang, Jumei Zhang, Yongdan Hu, Qingping Wu   +10 more
doaj   +1 more source