Results 31 to 40 of about 528,821 (173)

Establishment of a Simple and Rapid Nucleic Acid Detection Method for Hookworm Identification

Pathogens, 2023
Hookworm infection is one of the most common neglected tropical diseases and is mainly found in tropical and subtropical areas. Two species of human hookworm are distributed in China, i.e., Ancylostoma duodenale (AD) and Necator americanus (NA ...
Xin Ding, Yougui Yang, Yingshu Zhang, Qiang Zhang, Fanzhen Mao, Yang Dai   +5 more
doaj   +1 more source

Sensitive and Rapid Dual One-Tube Recombinase Aided PCR Assays for the Simultaneous Detection of Cytomegalovirus, Epstein-Barr Virus, Human Herpesvirus 6, and Herpes Simplex Virus 1/2 in Whole Blood From Children Undergoing Hematopoietic Stem Cell Transplantation. [PDF]

J Med Virol
ABSTRACT Early, rapid, and sensitive detection of cytomegalovirus (CMV), Epstein‐Barr virus (EBV), human herpesvirus 6 (HHV‐6), and herpes simplex virus Types 1/2 (HSV‐1/2) is critical for improving clinical outcomes in pediatric hematopoietic stem cell transplantation (HSCT).
Zhao L, Zhao M, Gao Y, Tie Y, Guo Y, Zhang S, Wang Y, Ma D, Shao Z, Feng Z.   +9 more
europepmc   +2 more sources

Research Note: Rapid detection of avian infectious laryngotracheitis virus with real-time fluorescence-based recombinase-aided amplification

Poultry Science, 2020
In this study, specific primers and fluorescent probes were designed to target the thymidine kinase (TK) gene sequence of avian infectious laryngotracheitis virus (ILTV).
Wenjing Wang, Chunguang Wang, Zichuang Zhang, Peng Zhang, Shanshan Yao, Jingru Liu, Xianghe Zhai, Tie Zhang   +7 more
doaj   +1 more source

A Recombinase-Aided Amplification Assay for the Detection of Chlamydia felis

Polish Journal of Microbiology, 2023
Chlamydia felis is an important zoonotic agent for humans and various animals. A recombinase-aided amplification (RAA) assay was developed for detecting C. felis. RAA can be performed in a closed tube at 39°C within 30 min.
Liu Jian, Qian Weidong, Wang Jian, Bai Yilan, Gui Yaping, Xia Luming, Gong Guohua, Ge Feifei, Shen Haixiao, Chang Xiaojing, Zhao Hongjin   +10 more
doaj   +1 more source

Development and evaluation of recombinase-aided amplification (RAA) assay for detecting Schistosoma haematobium gene in urine samples

, 2022
Abstract Background: A sensitive assay to diagnose S. haematobium infection (which causes urogenital schistosomiasis) is needed to improve control and elimination interventions. However, the sensitivity of immunological diagnostic methods and other methods (such as reagent strip testing to detect microhematuria and microscopy-based egg counting)
Song Zhao, Qiaoqiao Zhang, Xinyao Wang, Wei Li, Saleh Juma, Jianfeng Zhang, Kun Yang   +6 more
openaire   +1 more source

Clinical Validation of Two Recombinase-Based Isothermal Amplification Assays (RPA/RAA) for the Rapid Detection of African Swine Fever Virus

Frontiers in Microbiology, 2020
African swine fever (ASF), caused by African swine fever virus (ASFV), is a devastating infectious disease of domestic pigs and wild boars, and has tremendous negative socioeconomic impact on the swine industry and food security worldwide.
Xiaoxu Fan, Lin Li, Yonggang Zhao, Yutian Liu, Chunju Liu, Qinghua Wang, Yaqin Dong, Shujuan Wang, Tianying Chi, Fangfang Song, Chengyou Sun, Yingli Wang, Dengchuriya Ha, Yang Zhao, Yang Zhao, Jingyue Bao, Xiaodong Wu, Zhiliang Wang   +17 more
doaj   +1 more source

Recombinase-aided amplification–lateral flow dipstick assay—a specific and sensitive method for visual detection of avian infectious laryngotracheitis virus

Poultry Science, 2021
The purpose of this study was to explore a specific, simple, and sensitive method for diagnosis of avian infectious laryngotracheitis virus. Recombinase-aided amplification (RAA) and lateral flow dipstick (LFD) were combined for labeling the optimized ...
Wenjing Wang, Chunguang Wang, Zichuang Zhang, Peng Zhang, Xianghe Zhai, Xinyue Li, Tie Zhang   +6 more
doaj   +1 more source

Rapid visual detection of dengue virus by combining reverse transcription recombinase-aided amplification with lateral-flow dipstick assay

International Journal of Infectious Diseases, 2020
Objectives: Dengue caused by infection with the dengue virus (DENV) is endemic in the tropical and subtropical regions of the world and of greatest public health concern.
Yufeng Xiong, Yasha Luo, Huan Li, Weixiang Wu, Xiaolin Ruan, Xiaoping Mu   +5 more
doaj   +1 more source

Table_1_Establishment of a reverse transcription recombinase-aided amplification detection method for porcine group a rotavirus.DOCX

, 2022
Porcine rotavirus type A (PoRVA) is the main cause of dehydration and diarrhea in piglets, which has a great impact on the development of the pig industry worldwide. A rapid, accurate and sensitive detection method is conducive to the monitoring, control,
Yushun Wang (13796137), Xiangan Sun (13796140), Ling Zhu (56356), Zhiwen Xu (28724), Siyuan Lai (11993314), Huidan Deng (7330031), Yuancheng Zhou (418902), Mincai Nie (11906053)   +7 more
core   +1 more source

Establishment of a reverse transcription recombinase-aided amplification detection method for porcine group a rotavirus

Frontiers in Veterinary Science, 2022
Porcine rotavirus type A (PoRVA) is the main cause of dehydration and diarrhea in piglets, which has a great impact on the development of the pig industry worldwide. A rapid, accurate and sensitive detection method is conducive to the monitoring, control,
Yushun Wang, Mincai Nie, Huidan Deng, Siyuan Lai, Yuancheng Zhou, Yuancheng Zhou, Xiangan Sun, Ling Zhu, Ling Zhu, Zhiwen Xu, Zhiwen Xu   +10 more
doaj   +1 more source