Results 11 to 20 of about 528,821 (173)

Rapid Onsite Visual Detection of Orf Virus Using a Recombinase-Aided Amplification Assay

Life, 2023
Orf is an important zoonotic disease caused by the Orf virus (ORFV) which can cause contagious pustular dermatitis in goats and sheep. Orf is widespread in most sheep-raising countries in the world, causing huge economic losses.
Huan Cui, Jiyu Guan, Huijun Lu, Jun Liu, Fei Tu, Cheng Zhang, Kai Su, Zhendong Guo, Kui Zhao   +8 more
doaj   +4 more sources

Establishment of a Real-Time Reverse Transcription Recombinase-Aided Isothermal Amplification (qRT-RAA) Assay for the Rapid Detection of Bovine Respiratory Syncytial Virus

Veterinary Sciences
Background: Bovine respiratory syncytial virus (BRSV) is a significant cause of bovine respiratory disease, resulting in significant losses to the cattle industry. For rapid detection of BRSV, a real-time recombinase-aided isothermal amplification assay (
Guanxin Hou, Siping Zhu, Hong Li, Chihuan Li, Xiaochen Liu, Chao Ren, Xintong Zhu, Qiumei Shi, Zhiqiang Zhang   +8 more
doaj   +4 more sources

Recombinase-Aided Amplification Combined with Lateral Flow (LF-RAA) Assay for Rapid AAV Genome Detection

ACS Omega, 2022
Adeno-associated virus (AAV) is a versatile gene vector that is widely used in mammalian research. In basic studies and large-scale AAV production, genetic testing is ubiquitous and routine polymerase chain reaction (PCR)-based tests limit the efficiency due to the labor-intensive and time-consuming requirements of thermal cycling.
Kun Wang, Ruolan Huang, Liyun Zhang, Dan Liu, Yong Diao   +4 more
openaire   +3 more sources

Development and Application of Fluorescent and Lateral Flow Dipstick Recombinase-Aided Amplification for Rapid Detection of Glaesserella parasuis [PDF]

Veterinary Sciences
Glaesserella parasuis (G. parasuis) is a bacterial respiratory pathogen from pigs, which can cause high morbidity and mortality in weaned piglets in piggery. An accurate and rapid detection for G.
Yongliang Che, Yao Wang, Renjie Wu, Longbai Wang, Xuemin Wu, Qiuyong Chen, Rujing Chen, Lunjiang Zhou   +7 more
doaj   +3 more sources

Recombinase-Aided Amplification Coupled with Lateral Flow Dipstick for Efficient and Accurate Detection of Porcine Parvovirus

Life, 2021
Porcine parvovirus (PPV) infection is the primary cause of SMEDI (stillbirth; mummification; embryonic death; infertility) syndrome, which is a global burden for the swine industry.
Yihong He, Wenxian Chen, Jindai Fan, Shuangqi Fan, Hongxing Ding, Jinding Chen, Lin Yi   +6 more
doaj   +2 more sources

A rapid and sensitive recombinase aided amplification assay to detect hepatitis B virus without DNA extraction

BMC Infectious Diseases, 2019
Background Hepatitis B virus (HBV) infection is the major public health problem worldwide. In clinical practice, serological and molecular assays are the most commonly used diagnostic methods to detect HBV infection in clinical practices. Methods Here we
Xin-xin Shen, Fang-zhou Qiu, Li-Ping Shen, Ten-fei Yan, Meng-chuan Zhao, Ju-Ju Qi, Chen Chen, Li Zhao, Le Wang, Zhi-shan Feng, Xue-jun Ma   +10 more
doaj   +2 more sources

Establishment and Application Prospect of Reverse Transcriptase Recombinase-Aided Amplification Assay for Subgroup C Avian Metapneumovirus

Veterinary Sciences
Among broilers, the main pathogen that leads to swollen head syndrome (SHS) is the subgroup C avian metapneumovirus (aMPV-C). The aMPV-C infection can lead to an upsurge in the rate of soft-shell eggs, resulting in reduced egg production and seriously ...
Yuhang Bai, Xiuhong Wu, Jiajia Liu, Zhanxin Wang, Mengyue Dong, Tong Li, Zhenkai Dai, Hongxin Li, Qingmei Xie, Xinheng Zhang   +9 more
doaj   +2 more sources

Rapid detection of Mycoplasma hyopneumoniae by recombinase-aided amplification combined with the CRISPR/Cas12a system [PDF]

Frontiers in Cellular and Infection Microbiology
Mycoplasma hyopneumoniae (M. hyopneumoniae) is one of the primary agents involved in porcine respiratory disease complex, and circulates in the swine industry worldwide. The prevention and control of M. hyopneumoniae is complicated.
Kaili Li, Kaili Li, Kaili Li, Tingyu Luo, Tingyu Luo, Tingyu Luo, Yu Zhang, Yu Zhang, Yu Zhang, Changwen Li, Changwen Li, Changwen Li, Hongyan Chen, Hongyan Chen, Hongyan Chen, Changyou Xia, Changyou Xia, Changyou Xia, Caixia Gao, Caixia Gao, Caixia Gao   +20 more
doaj   +2 more sources

Rapid and Highly Sensitive Detection of Mycobacterium tuberculosis Utilizing the Recombinase Aided Amplification-Based CRISPR-Cas13a System

Microorganisms
Tuberculosis (TB), a disease caused by Mycobacterium tuberculosis (MTB) infection, remains a major threat to global public health. To facilitate early TB diagnosis, an IS6110 gene-based recombinase aided amplification (RAA) assay was coupled to a ...
Qiao Li, Nenhan Wang, Mengdi Pang, Honghao Miao, Xiaowei Dai, Bo Li, Xinyu Yang, Chuanyou Li, Yi Liu   +8 more
doaj   +2 more sources

Rapid and visual field diagnosis of tomato brown rugose fruit virus using reverse transcription recombinase aided amplification (RT RAA) combined with lateral flow strips (LFS)

Crop Protection, 2023
Fei Yan, Junmin Li, Yuhao Cao
exaly   +2 more sources