Results 1 to 10 of about 520,105 (221)

Rapid detection of Mycoplasma hyopneumoniae by recombinase-aided amplification combined with the CRISPR/Cas12a system [PDF]

open access: yesFrontiers in Cellular and Infection Microbiology
Mycoplasma hyopneumoniae (M. hyopneumoniae) is one of the primary agents involved in porcine respiratory disease complex, and circulates in the swine industry worldwide. The prevention and control of M. hyopneumoniae is complicated.
Kaili Li   +20 more
doaj   +8 more sources

Rapid Onsite Visual Detection of Orf Virus Using a Recombinase-Aided Amplification Assay

open access: yesLife, 2023
Orf is an important zoonotic disease caused by the Orf virus (ORFV) which can cause contagious pustular dermatitis in goats and sheep. Orf is widespread in most sheep-raising countries in the world, causing huge economic losses.
Huan Cui   +8 more
doaj   +6 more sources

A Recombinase-Aided Amplification Assay for the Detection of Chlamydia felis [PDF]

open access: yesPolish Journal of Microbiology, 2023
Chlamydia felis is an important zoonotic agent for humans and various animals. A recombinase-aided amplification (RAA) assay was developed for detecting C. felis. RAA can be performed in a closed tube at 39°C within 30 min.
Liu Jian   +10 more
doaj   +4 more sources

Rapid detection of mpox virus using recombinase aided amplification assay [PDF]

open access: yesFrontiers in Cellular and Infection Microbiology, 2023
A recent, unprecedented outbreak of human mpox virus infection has led to cases in non-African nations, and the number of confirmed or suspected cases outside of Africa has exceeded 1,000 within 5 weeks.
Xiaohu Cui   +19 more
doaj   +4 more sources

Advances in the application of recombinase-aided amplification combined with CRISPR-Cas technology in quick detection of pathogenic microbes [PDF]

open access: yesFrontiers in Bioengineering and Biotechnology, 2023
The rapid diagnosis of pathogenic infections plays a vital role in disease prevention, control, and public health safety. Recombinase-aided amplification (RAA) is an innovative isothermal nucleic acid amplification technology capable of fast DNA or RNA ...
Xiaoping Li   +16 more
doaj   +4 more sources

Reverse Transcription Recombinase-Aided Amplification Assay for Newcastle Disease Virus in Poultry [PDF]

open access: yesPathogens
Newcastle disease (ND) is a highly contagious and economically significant viral infection that affects poultry globally, with recurrent outbreaks occurring even among vaccinated flocks in Egypt.
Nahed Yehia   +8 more
doaj   +4 more sources

Establishment of reverse transcription recombinase–aided amplification-lateral-flow dipstick and real-time fluorescence–based reverse transcription recombinase–aided amplification methods for detection of the Newcastle disease virus in chickens

open access: yesPoultry Science, 2020
Newcastle disease is an acute and highly contagious disease of poultry caused by Newcastle disease virus infection, which does great harm to the poultry industry all over the world.
Wenjing Wang   +6 more
doaj   +5 more sources

Fast detection of duck circovirus by real-time fluorescence-based recombinase-aided amplification

open access: yesPoultry Science, 2022
: Duck circovirus disease (DuCVD), as an immunosuppressive disease, is a threat to the poultry industry. In order to diagnose this disease quickly and accurately, a real-time fluorescence-based recombinase-aided amplification (RF-RAA) method was ...
Xinyue Li   +9 more
doaj   +5 more sources

Detection of the mpox virus using a robust recombinase-aided amplification-based approach [PDF]

open access: yesBiosafety and Health
In 2022, a global outbreak of mpox was anticipated, with several cases reported in non-endemic countries in early May. Given the challenge of distinguishing the mpox virus (MPXV) from other pathogens based solely on symptoms, there is an urgent need for ...
Meihui Luo   +22 more
doaj   +4 more sources

A Review of the Application of Recombinase Polymerase Amplification, Recombinase-Aided Amplification and Enzymatic Recombinase Amplification in Rapid Detection of Foodborne Pathogens [PDF]

open access: yesShipin Kexue, 2023
With the improvement of people’s living standards, food safety has attracted more and more social attention. Organisms, especially microorganisms, are the most important factor that affects food safety.
WANG Shuai, YANG Yange, WU Zhanwen, LI Hongna, LI Tao, SUN Dongmei, YUAN Fei
doaj   +2 more sources

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