Results 31 to 40 of about 520,105 (221)

Enhancement of Recombinase-Aided Amplification (Raa) Assay by Betaine and Pullulan [PDF]

, 2021
Abstract In this research, nucleic acid amplification enhancers suitable for recombinase-aided amplification (RAA) assay were studied for the first time, and amplification of a long-fragment (509 bp) by the RAA assay was initially explored. Using recombinant plasmids and clinical samples, RAA fluorescence and basic methods were used to evaluate
Jinrong Wang, Guowei Song, Yue Ming, Jing Pan, Ruiqing Zhang, Guohao Fan, Yuan Gao, Xinxin Shen, Xuejun Ma, Lixin Li   +9 more
openaire   +1 more source

Research Note: Rapid detection of avian infectious laryngotracheitis virus with real-time fluorescence-based recombinase-aided amplification

Poultry Science, 2020
In this study, specific primers and fluorescent probes were designed to target the thymidine kinase (TK) gene sequence of avian infectious laryngotracheitis virus (ILTV).
Wenjing Wang, Chunguang Wang, Zichuang Zhang, Peng Zhang, Shanshan Yao, Jingru Liu, Xianghe Zhai, Tie Zhang   +7 more
doaj   +1 more source

Sensitive and specific exonuclease III-assisted recombinase-aided amplification colorimetric assay for rapid detection of nucleic acids

, 2023
The development of a contamination-free and on-site nucleic acid detection platform with high sensitivity and specificity but low-cost for the detection of pathogenic nucleic acids is critical for infectious disease diagnosis and surveillance.
Tao, D., Zhao, S., Steinaa, Lucilla, Sun, H., Ruan, J., Li, X., Han Jianlin, Ren, R., Hemmink, Johanneke D., Xu, B., Zhao, C., Han, X., Xie, S., Xu, J., Zhou, Y., Zhang, J.   +15 more
core   +3 more sources

Rapid Detection of New Delhi Metallo-β-Lactamase Gene Using Recombinase-Aided Amplification Directly on Clinical Samples From Children

Frontiers in Microbiology, 2021
New Delhi metallo-β-lactamase, a metallo-β-lactamase carbapenemase type, mediates resistance to most β-lactam antibiotics including penicillins, cephalosporins, and carbapenems.
Yanling Feng, Guanhua Xue, Junxia Feng, Chao Yan, Jinghua Cui, Lin Gan, Rui Zhang, Hanqin Zhao, Wenjian Xu, Nannan Li, Shiyu Liu, Shuheng Du, Weiwei Zhang, Hailan Yao, Jun Tai, Lijuan Ma, Ting Zhang, Dong Qu, Yongxiang Wei, Jing Yuan   +19 more
doaj   +1 more source

Recombinase-Aided Amplification Combined with Lateral Flow (LF‑RAA) Assay for Rapid AAV Genome Detection

, 2022
Adeno-associated virus (AAV) is a versatile gene vector that is widely used in mammalian research. In basic studies and large-scale AAV production, genetic testing is ubiquitous and routine polymerase chain reaction (PCR)-based tests limit the efficiency
Yong Diao (524165), Ruolan Huang (14266006), Dan Liu (108669), Liyun Zhang (280172), Kun Wang (134525)   +4 more
core   +1 more source

Establishment of two assays based on reverse transcription recombinase-aided amplification technology for rapid detection of H5 subtype avian influenza virus

Microbiology Spectrum, 2023
The avian influenza virus (AIV), a member of the type A influenza virus group, is harbored by avian hosts. The highly pathogenic H5 subtype of AIV has inflicted significant financial damage on the poultry industry and also presents a possible pandemic ...
Yang Li, Jiajing Shang, Yixin Wang, Juan Luo, Wenming Jiang, Xin Yin, Fuyou Zhang, Chunran Deng, Xiaohui Yu, HuaLei Liu   +9 more
doaj   +1 more source

Establishment of a Simple and Rapid Nucleic Acid Detection Method for Hookworm Identification

Pathogens, 2023
Hookworm infection is one of the most common neglected tropical diseases and is mainly found in tropical and subtropical areas. Two species of human hookworm are distributed in China, i.e., Ancylostoma duodenale (AD) and Necator americanus (NA ...
Xin Ding, Yougui Yang, Yingshu Zhang, Qiang Zhang, Fanzhen Mao, Yang Dai   +5 more
doaj   +1 more source

DataSheet1_On-site and visual detection of sorghum mosaic virus and rice stripe mosaic virus based on reverse transcription-recombinase-aided amplification and CRISPR/Cas12a.docx

, 2023
Rapid, sensitive and visual detection of plant viruses is conducive to effective prevention and control of plant viral diseases. Therefore, combined with reverse transcription and recombinase-aided amplification, we developed a CRISPR/Cas12a-based visual
Tong Zhang (103827), Guohui Zhou (3692), Junkai Wang (3092706), Zhengyi Liang (10106595), Xiuqin Huang (8049470), Siping Chen (296896), Biao Chen (795331), Xin Yang (104842), Jiahao Chen (194408)   +8 more
core   +1 more source

Clinical Validation of Two Recombinase-Based Isothermal Amplification Assays (RPA/RAA) for the Rapid Detection of African Swine Fever Virus

Frontiers in Microbiology, 2020
African swine fever (ASF), caused by African swine fever virus (ASFV), is a devastating infectious disease of domestic pigs and wild boars, and has tremendous negative socioeconomic impact on the swine industry and food security worldwide.
Xiaoxu Fan, Lin Li, Yonggang Zhao, Yutian Liu, Chunju Liu, Qinghua Wang, Yaqin Dong, Shujuan Wang, Tianying Chi, Fangfang Song, Chengyou Sun, Yingli Wang, Dengchuriya Ha, Yang Zhao, Yang Zhao, Jingyue Bao, Xiaodong Wu, Zhiliang Wang   +17 more
doaj   +1 more source

Critical insight into recombinase polymerase amplification technology

, 2022
Introduction Recombinase polymerase amplification (RPA) is a promising and emerging technology for rapidly amplifying target nucleic acid from minimally processed samples and through small portable instruments. RPA is suitable for point-of-care testing (
Munawar, Mustafa Ahmad, Mustafa Ahmad Munawar   +1 more
core   +1 more source