Results 21 to 30 of about 520,105 (221)

A rapid and sensitive recombinase aided amplification assay to detect hepatitis B virus without DNA extraction

open access: yesBMC Infectious Diseases, 2019
Background Hepatitis B virus (HBV) infection is the major public health problem worldwide. In clinical practice, serological and molecular assays are the most commonly used diagnostic methods to detect HBV infection in clinical practices. Methods Here we
Xin-xin Shen   +10 more
doaj   +2 more sources

Dye‐based recombinase‐aided amplification assay with enhanced sensitivity and specificity

open access: yesiLABMED
Background Fluorescent recombinase‐aided amplification (RAA) assays are increasingly being used in the detection of a variety of pathogens and have the advantages of rapidity and simplicity and similar sensitivity and specificity, compared with real‐time
Zijin Zhao   +5 more
doaj   +2 more sources

Development and Application of a Reverse-Transcription Recombinase-Aided Amplification Assay for Porcine Epidemic Diarrhea Virus

open access: yesViruses, 2022
Porcine epidemic diarrhea virus (PEDV) is a coronavirus currently widespread worldwide in the swine industry. Since PEDV was discovered in China in 1984, it has caused huge economic losses in the swine industry. PEDV can infect pigs of all ages, but piglets have the highest infection with a death rate as high as 100%, and the clinical symptoms are ...
Xiuhong Wu   +7 more
openaire   +4 more sources

Development of a real-time recombinase-aided amplification assay for rapid and sensitive detection of Edwardsiella piscicida [PDF]

open access: yesFrontiers in Cellular and Infection Microbiology
Edwardsiella piscicida, a significant intracellular pathogen, is widely distributed in aquatic environments and causes systemic infection in various species.
Yuchen Dong   +4 more
doaj   +2 more sources

Rapid Visual Detection of Plasmodium Using Recombinase-Aided Amplification With Lateral Flow Dipstick Assay

open access: yesFrontiers in Cellular and Infection Microbiology, 2022
BackgroundMalaria is a global public health problem. China has had no case of indigenous malaria since 2016. However, imported cases of malaria remain an issue among travelers, overseas workers, and foreign traders.
Hong Lin   +6 more
doaj   +3 more sources

A rapid and visual detection assay for Senecavirus A based on recombinase-aided amplification and lateral flow dipstick. [PDF]

open access: yesFront Cell Infect Microbiol
BackgroundSenecavirus A (SVA) is a newly pathogenic virus correlated with the acute death of piglets and vesicular lesions in pigs. The further prevalence of SVA will cause considerable economic damage to the global pig farming industry. Therefore, rapid and accurate diagnostic tools for SVA are crucial for preventing and controlling the disease ...
Song Y   +12 more
europepmc   +4 more sources

Research Note: Real-time fluorescence-based recombinase-aided amplification for rapid detection of Mycoplasma synoviae [PDF]

open access: yesPoultry Science
: Mycoplasma synoviae (MS) is an essential pathogenic mycoplasma in poultry worldwide, posing a serious threat to the poultry industry's health. Timely detection is imperative for early diagnosis, prevention, and control of MS infection.
Wenlong Xia   +9 more
doaj   +2 more sources

Development of a Lateral Flow Strip-Based Recombinase-Aided Amplification for Active Chlamydia psittaci Infection

open access: yesFrontiers in Microbiology, 2022
Chlamydia psittaci is the causative agent of psittacosis, a worldwide zoonotic disease. A rapid, specific, and sensitive diagnostic assay would be benefit for C. psittaci infection control.
Jun Jiao   +7 more
doaj   +3 more sources

RAP: A Novel Approach to the Rapid and Highly Sensitive Detection of Respiratory Viruses

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
Recombinase aided amplification (RAA) is an emerging isothermal amplification method used for detecting various pathogens. However, RAA requires a complex and long probe to ensure high sensitivity during fluorescence assay.
Guohao Fan   +7 more
doaj   +1 more source

A Fluorescent Recombinase Aided Amplification Assay for Detection of Babesia microti

open access: yesThe Korean Journal of Parasitology, 2022
<i>Babesia microti</i> is one of the most common causative agents of babesiosis. A sensitive and rapid detection is necessary for screening potentially infected individuals. In this study, <i>B. microti</i> cytochrome <i>c</i> oxidase subunit I (<i>cox</i>1) was selected as the target gene, multiple ...
Hong Lin   +5 more
openaire   +2 more sources

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