Results 1 to 10 of about 10,703 (255)
Temperate Phages Acquire DNA from Defective Prophages by Relaxed Homologous Recombination: The Role of Rad52-Like Recombinases [PDF]
PLoS Genetics, 2014 Bacteriophages (or phages) dominate the biosphere both numerically and in terms of genetic diversity. In particular, genomic comparisons suggest a remarkable level of horizontal gene transfer among temperate phages, favoring a high evolution rate ...
Marianne De Paepe +2 more
exaly +2 more sources
High-performance chemical- and light-inducible recombinases in mammalian cells and mice
Nature Communications, 2019 The availability of high performance recombinases with low basal activity and high dynamic range is limited. Here the authors present a library of over 20 orthogonal split recombinases that can be induced by small molecules, light and temperature in vivo.
Benjamin H Weinberg +2 more
exaly +2 more sources
Redesigning Recombinase Specificity for Safe Harbor Sites in the Human Genome. [PDF]
PLoS ONE, 2015 Site-specific recombinases (SSRs) are valuable tools for genetic engineering due to their ability to manipulate DNA in a highly specific manner. Engineered zinc-finger and TAL effector recombinases, in particular, are two classes of SSRs composed of ...
Mark C Wallen +2 more
doaj +1 more source
Computational characterization of recombinase circuits for periodic behaviors
iScience, 2023 Summary: Recombinases are site-specific proteins found in nature that are capable of rearranging DNA. This function has made them promising gene editing tools in synthetic biology, as well as key elements in complex artificial gene circuits implementing ...
Judith Landau +3 more
doaj +1 more source
Prediction of designer-recombinases for DNA editing with generative deep learning
Nature Communications, 2022 Design of recombinases with new target sites is usually achieved through cycles of directed molecular evolution. Here the authors report Recombinase Generator, RecGen, an algorithm for generation of designer-recombinases; they perform experimental ...
Lukas Theo Schmitt +3 more
doaj +1 more source
Site‐specific recombinase genome engineering toolkit in maize
Plant Direct, 2020 Site‐specific recombinase enzymes function in heterologous cellular environments to initiate strand‐switching reactions between unique DNA sequences termed recombinase binding sites. Depending on binding site position and orientation, reactions result in
Jon P. Cody +4 more
doaj +1 more source
Frontiers in Neuroanatomy, 2017 Central noradrenergic neurons, collectively defined by synthesis of the neurotransmitter norepinephrine, are a diverse collection of cells in the hindbrain, differing in their anatomy, physiological and behavioral functions, and susceptibility to disease
Nicholas W. Plummer +4 more
doaj +1 more source
Stimulation of homologous recombination in plants expressing heterologous recombinases
BMC Plant Biology, 2020 Background Current excitement about the opportunities for gene editing in plants have been prompted by advances in CRISPR/Cas and TALEN technologies. CRISPR/Cas is widely used to knock-out or modify genes by inducing targeted double-strand breaks (DSBs ...
Abdellah Barakate +3 more
doaj +1 more source
Molecules, 2023 RecA family recombinases are the core enzymes in the process of homologous recombination, and their normal operation ensures the stability of the genome and the healthy development of organisms.
Yue Pan +4 more
doaj +1 more source
Manipulating or superseding host recombination functions: a dilemma that shapes phage evolvability. [PDF]
PLoS Genetics, 2013 Phages, like many parasites, tend to have small genomes and may encode autonomous functions or manipulate those of their hosts'. Recombination functions are essential for phage replication and diversification. They are also nearly ubiquitous in bacteria.
Louis-Marie Bobay +2 more
doaj +1 more source