Temperate Phages Acquire DNA from Defective Prophages by Relaxed Homologous Recombination: The Role of Rad52-Like Recombinases [PDF]
PLoS Genetics, 2014 Bacteriophages (or phages) dominate the biosphere both numerically and in terms of genetic diversity. In particular, genomic comparisons suggest a remarkable level of horizontal gene transfer among temperate phages, favoring a high evolution rate ...
Marianne De Paepe +2 more
exaly +2 more sources
High-performance chemical- and light-inducible recombinases in mammalian cells and mice
Nature Communications, 2019 The availability of high performance recombinases with low basal activity and high dynamic range is limited. Here the authors present a library of over 20 orthogonal split recombinases that can be induced by small molecules, light and temperature in vivo.
Wilson W Wong +2 more
exaly +2 more sources
From Gene Knockouts to Genome Remodeling: Large DNA Fragment Deletion Technologies in Plants [PDF]
PlantsLarge DNA fragment deletion (LDFD) provides a powerful means to reconfigure plant genomes at the kilobase to megabase scale, enabling the dissection of genome function, elucidation of non-coding regulatory elements, modulation of gene dosage ...
Jiayi Hou +6 more
doaj +2 more sources
Single-cell characterization of bacterial optogenetic Cre recombinases [PDF]
iScienceSummary: Microbial optogenetic tools can regulate gene expression with spatial and temporal precision, offering excellent potential for single-cell resolution studies.
Hellen Huang +2 more
doaj +2 more sources
Minimal mutational requirements for conversion of a telomere resolvase into a Cre-like site-specific recombinase. [PDF]
PLoS ONEHairpin telomere resolvases comprise a family of enzymes that produce the hairpin (hp) telomeres of bacteria and their phages that possess linear chromosomes and plasmids terminated by covalently closed hp telomeres.
Shu Hui Huang, Kerri Kobryn
doaj +2 more sources
Zinc Finger Recombinases with Adaptable DNA Sequence Specificity
PLoS ONE, 2011 Site-specific recombinases have become essential tools in genetics and molecular biology for the precise excision or integration of DNA sequences. However, their utility is currently limited to circumstances where the sites recognized by the recombinase ...
Marshall Stark +2 more
exaly +3 more sources
RECC: A Red/ET–CRISPR/Cas9-based system enabling genome mining of marine Pseudoalteromonas for novel natural products [PDF]
Synthetic and Systems BiotechnologyMarine microorganisms possess vast biosynthetic potential, yet most of their biosynthetic gene clusters (BGCs) remain transcriptionally silent under laboratory conditions.
Zong-jie Wang +5 more
doaj +2 more sources
Redesigning Recombinase Specificity for Safe Harbor Sites in the Human Genome. [PDF]
PLoS ONE, 2015 Site-specific recombinases (SSRs) are valuable tools for genetic engineering due to their ability to manipulate DNA in a highly specific manner. Engineered zinc-finger and TAL effector recombinases, in particular, are two classes of SSRs composed of ...
Mark C Wallen +2 more
doaj +1 more source
Prediction of designer-recombinases for DNA editing with generative deep learning
Nature Communications, 2022 Design of recombinases with new target sites is usually achieved through cycles of directed molecular evolution. Here the authors report Recombinase Generator, RecGen, an algorithm for generation of designer-recombinases; they perform experimental ...
Lukas Theo Schmitt +3 more
doaj +1 more source
Site‐specific recombinase genome engineering toolkit in maize
Plant Direct, 2020 Site‐specific recombinase enzymes function in heterologous cellular environments to initiate strand‐switching reactions between unique DNA sequences termed recombinase binding sites. Depending on binding site position and orientation, reactions result in
Jon P. Cody +4 more
doaj +1 more source