Results 11 to 20 of about 10,703 (255)
Single-cell characterization of bacterial optogenetic Cre recombinases [PDF]
iScienceSummary: Microbial optogenetic tools can regulate gene expression with spatial and temporal precision, offering excellent potential for single-cell resolution studies.
Hellen Huang +2 more
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From Gene Knockouts to Genome Remodeling: Large DNA Fragment Deletion Technologies in Plants [PDF]
PlantsLarge DNA fragment deletion (LDFD) provides a powerful means to reconfigure plant genomes at the kilobase to megabase scale, enabling the dissection of genome function, elucidation of non-coding regulatory elements, modulation of gene dosage ...
Jiayi Hou +6 more
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RECC: A Red/ET–CRISPR/Cas9-based system enabling genome mining of marine Pseudoalteromonas for novel natural products [PDF]
Synthetic and Systems BiotechnologyMarine microorganisms possess vast biosynthetic potential, yet most of their biosynthetic gene clusters (BGCs) remain transcriptionally silent under laboratory conditions.
Zong-jie Wang +5 more
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Archaeal tyrosine recombinases
FEMS Microbiology Reviews, 2021 ABSTRACTThe integration of mobile genetic elements into their host chromosome influences the immediate fate of cellular organisms and gradually shapes their evolution. Site-specific recombinases catalyzing this integration have been extensively characterized both in bacteria and eukarya.
Badel, Catherine +2 more
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Gene stacking by recombinases [PDF]
Plant Biotechnology Journal, 2015 SummaryEfficient methods of stacking genes into plant genomes are needed to expedite transfer of multigenic traits to crop varieties of diverse ecosystems. Over two decades of research has identified several DNA recombinases that carryout efficient cis and trans recombination between the recombination sites artificially introduced into the plant ...
Vibha, Srivastava, James, Thomson
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RAG: a recombinase diversified [PDF]
Nature Immunology, 2009 During B cell and T cell development, the lymphoid-specific proteins RAG-1 and RAG-2 act together to initiate the assembly of antigen receptor genes through a series of site-specific somatic DNA rearrangements that are collectively called variable-diversity-joining (V(D)J) recombination.
Adam G W, Matthews +1 more
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mSphere, 2019 Most urinary tract infections (UTIs) are caused by uropathogenic Escherichia coli (UPEC), which depends on an extracellular organelle (type 1 pili) for adherence to bladder cells during infection.
Colin W. Russell +6 more
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BRCA2: a universal recombinase regulator [PDF]
Oncogene, 2007 Homologous recombination has a dual role in eukaryotic organisms. Firstly, it is responsible for the creation of genetic variability during meiosis by directing the formation of reciprocal crossovers that result in random combinations of alleles and traits.
T, Thorslund, S C, West
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Cell Reports, 2020 Summary: Meiotic recombination is critical for genetic exchange and generation of chiasmata that ensures faithful chromosome segregation during meiosis I.
Kazumasa Takemoto +9 more
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The emerging role of recombineering in microbiology
Engineering Microbiology, 2023 : Recombineering is a valuable technique for generating recombinant DNA in vivo, primarily in bacterial cells, and is based on homologous recombination using phage-encoded homologous recombinases, such as Redαβγ from the lambda phage and RecET from the ...
Ruijuan Li +3 more
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