Results 41 to 50 of about 12,788 (278)
eLife, 2022 Following infection of bacterial cells, bacteriophage modulate double-stranded DNA break repair pathways to protect themselves from host immunity systems and prioritise their own recombinases.
Martin Wilkinson +5 more
doaj +1 more source
Correction of a Factor VIII genomic inversion with designer-recombinases [PDF]
Despite advances in nuclease-based genome editing technologies, correcting human disease-causing genomic inversions remains a challenge. Here, we describe the potential use of a recombinase-based system to correct the 140 kb inversion of the F8 gene ...
Buchholz, Frank +16 more
core +1 more source
Characterization of an archaeal recombinase paralog that exhibits novel anti-recombinase activity
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 2020 The process of homologous recombination is heavily dependent on the RecA family of recombinases for repair of DNA double-strand breaks. These recombinases are responsible for identifying homologies and forming heteroduplex DNA between substrate ssDNA and dsDNA templates, activities that are modified by various accessory factors.
Corey, Knadler +3 more
openaire +3 more sources
Alignment of Homologous Chromosomes and Effective Repair of Programmed DNA Double-Strand Breaks during Mouse Meiosis Require the Minichromosome Maintenance Domain Containing 2 (MCMDC2) Protein. [PDF]
PLoS Genetics, 2016 Orderly chromosome segregation during the first meiotic division requires meiotic recombination to form crossovers between homologous chromosomes (homologues).
Friederike Finsterbusch +5 more
doaj +1 more source
PLoS Genetics, 2022 Recombinases RAD51 and its meiosis-specific paralog DMC1 accumulate on single-stranded DNA (ssDNA) of programmed DNA double strand breaks (DSBs) in meiosis.
Lieke Koornneef +8 more
doaj +1 more source
Microbiology Spectrum, 2015 ABSTRACT The use of Cre recombinase to carry out conditional mutagenesis of transgenes and insert DNA cassettes into eukaryotic chromosomes is widespread. In addition to the numerous in vivo and in vitro applications that have been reported since Cre was first shown to function in ...
openaire +2 more sources
RNA‐related functions of BRCA2 in transcription, replication, and genome stability
FEBS Letters, EarlyView.Beyond its role in homologous recombination, BRCA2 regulates R‐loop homeostasis, DNA–RNA hybrid resolution, RNA polymerase II dynamics, and transcriptional activation. Disruption of any of these functions can contribute to genome instability, inflammatory signaling, and tumorigenesis. BRCA2 is a central tumor suppressor protein best known for its roles
Lucia Alvaro‐Aranda +2 more
wiley +1 more source
Directed evolution of site-specific recombinases for precise genome editing and rearrangement [PDF]
, 2021 The Cre/loxP system belongs to the family of site-specific recombinases (SSR) that can precisely modify DNA that is flanked by two target sites. The reaction outcome is dependent on the structure and orientation of the target sites and includes excision,
Lansing, Felix Johannes
core +3 more sources
PLoS ONE, 2013 Tyrosine recombinases are conserved in the three kingdoms of life. Here we present the first crystal structure of a full-length archaeal tyrosine recombinase, XerA from Pyrococcus abyssi, at 3.0 Å resolution.
Marie-Claude Serre +8 more
doaj +1 more source
Microbiology Spectrum, 2014 ABSTRACTIn site-specific recombination, two short DNA sequences (‘sites’) are each cut at specific points in both strands, and the cut ends are rejoined to new partners. The enzymes that mediate recognition of the sites and the subsequent cutting and rejoining steps are called recombinases.
openaire +2 more sources