Results 41 to 50 of about 10,769 (161)
PLoS ONE, 2013 Tyrosine recombinases are conserved in the three kingdoms of life. Here we present the first crystal structure of a full-length archaeal tyrosine recombinase, XerA from Pyrococcus abyssi, at 3.0 Å resolution.
Marie-Claude Serre +8 more
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Alignment of Homologous Chromosomes and Effective Repair of Programmed DNA Double-Strand Breaks during Mouse Meiosis Require the Minichromosome Maintenance Domain Containing 2 (MCMDC2) Protein. [PDF]
PLoS Genetics, 2016 Orderly chromosome segregation during the first meiotic division requires meiotic recombination to form crossovers between homologous chromosomes (homologues).
Friederike Finsterbusch +5 more
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Microbiology Spectrum, 2014 ABSTRACTIn site-specific recombination, two short DNA sequences (‘sites’) are each cut at specific points in both strands, and the cut ends are rejoined to new partners. The enzymes that mediate recognition of the sites and the subsequent cutting and rejoining steps are called recombinases.
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Serine integrase chimeras with activity in E. coli and HeLa cells
Biology Open, 2014 In recent years, application of serine integrases for genomic engineering has increased in popularity. The factor-independence and unidirectionality of these large serine recombinases makes them well suited for reactions such as site-directed vector ...
Alfonso P. Farruggio, Michele P. Calos
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A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination
Scientific Reports, 2018 Site-specific recombinases (SSR) are utilized as important genome engineering tools to precisely modify the genome of mice and other model organisms. Reporter mice that mark cells that at any given time had expressed the enzyme are frequently used for ...
Madina Karimova +5 more
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Recombinase technology: applications and possibilities [PDF]
Plant Cell Reports, 2010 The use of recombinases for genomic engineering is no longer a new technology. In fact, this technology has entered its third decade since the initial discovery that recombinases function in heterologous systems (Sauer in Mol Cell Biol 7(6):2087-2096, 1987).
Wang, Yueju +3 more
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Frontiers in Genetics, 2018 The RAD51 (DNA repair protein RAD51) recombinases are essential for homologous recombination, DNA repair, and genome stability. Overexpression of RAD51 proteins has been observed in many cancer cells, such as thyroid carcinoma, breast cancer, pancreatic ...
Shan Jiang +3 more
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Herpes ICP8 protein stimulates homologous recombination in human cells. [PDF]
PLoS ONE, 2018 Recombineering has transformed functional genomic analysis. Genome modification by recombineering using the phage lambda Red homologous recombination protein Beta in Escherichia coli has approached 100% efficiency. While highly efficient in E.
Melvys Valledor +2 more
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Multiple interfaces between a serine recombinase and an enhancer control site-specific DNA inversion
eLife, 2013 Serine recombinases are often tightly controlled by elaborate, topologically-defined, nucleoprotein complexes. Hin is a member of the DNA invertase subclass of serine recombinases that are regulated by a remote recombinational enhancer element containing
Meghan M McLean +4 more
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BRCA2: a universal recombinase regulator [PDF]
Oncogene, 2007 Homologous recombination has a dual role in eukaryotic organisms. Firstly, it is responsible for the creation of genetic variability during meiosis by directing the formation of reciprocal crossovers that result in random combinations of alleles and traits.
T, Thorslund, S C, West
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