Results 61 to 70 of about 12,788 (278)
Frontiers in Genetics, 2018 The RAD51 (DNA repair protein RAD51) recombinases are essential for homologous recombination, DNA repair, and genome stability. Overexpression of RAD51 proteins has been observed in many cancer cells, such as thyroid carcinoma, breast cancer, pancreatic ...
Shan Jiang +3 more
doaj +1 more source
The RAG recombinase: Beyond breaking [PDF]
Mechanisms of Ageing and Development, 2017 DNA double-strand breaks (DSBs) are commonly seen as lesions that threaten genome integrity and contribute to cancer and aging processes. However, in the context of antigen receptor gene assembly, known as V(D)J recombination, DSBs are obligatory intermediates that allow the establishment of genetic diversity and adaptive immunity.
Lescale, Chloé, Deriano, Ludovic
openaire +3 more sources
Advanced Materials, EarlyView.A cholesterol‐conjugated lipid library enabled the identification of a ligand‐free LNP platform for efficient mRNA delivery to brain endothelial cells via systemic administration. This platform achieves selective BBB targeting without disrupting barrier integrity, and enables modulation of neuroinflammation and vascular function without requiring trans‐
Zeru Tian +7 more
wiley +1 more source
Herpes ICP8 protein stimulates homologous recombination in human cells. [PDF]
PLoS ONE, 2018 Recombineering has transformed functional genomic analysis. Genome modification by recombineering using the phage lambda Red homologous recombination protein Beta in Escherichia coli has approached 100% efficiency. While highly efficient in E.
Melvys Valledor +2 more
doaj +1 more source
Advanced Science, EarlyView.ABSTRACT Astrocyte reactivity critically shapes neuroinflammatory outcomes after ischemic stroke, yet the upstream regulators governing astrocyte state transitions remain incompletely defined. Here, we identify the immunoproteasome subunit low molecular weight protein 2 (LMP2) as an important modulator of astrocyte functional remodeling following ...
Yanguang Mao +7 more
wiley +1 more source
Advanced Science, EarlyView.ABSTRACT Amplification‐free Cas12a diagnostics with split crRNA enable rapid and programmable target recognition, yet insufficient understanding of DNA activator architecture prevents predictable control over trans‐cleavage activity and sensitivity. Here we systematically map over 200 split DNA activator configurations by introducing nicks at every ...
Xiaoyan Tang +8 more
wiley +1 more source
Multiple interfaces between a serine recombinase and an enhancer control site-specific DNA inversion
eLife, 2013 Serine recombinases are often tightly controlled by elaborate, topologically-defined, nucleoprotein complexes. Hin is a member of the DNA invertase subclass of serine recombinases that are regulated by a remote recombinational enhancer element containing
Meghan M McLean +4 more
doaj +1 more source
Chimeric recombinases with designed DNA sequence recognition
, 2003 Site-specific recombination typically occurs only between DNA sequences that have co-evolved with a natural recombinase enzyme to optimize sequence recognition, catalytic efficiency, and regulation.
Akopian, A +3 more
core +1 more source
Engineering chimaeric recombinases for HIV-1 proviral DNA excision [PDF]
, 2018 ‘Cutting out’ HIV-1 proviral DNA could potentially cure a person of the infection. Genome editing approaches have been proffered for eradicating the provirus in infected persons by activating all latent viral reservoirs for further antiretroviral therapy
Abioye, Jumai Adeola
core +1 more source
Molecular Evolution of the Tre Recombinase [PDF]
Journal of Visualized Experiments, 2008 Here we report the generation of Tre recombinase through directed, molecular evolution. Tre recombinase recognizes a pre-defined target sequence within the LTR sequences of the HIV-1 provirus, resulting in the excision and eradication of the provirus from infected human cells.
openaire +2 more sources