Results 61 to 70 of about 10,769 (161)

Monomer Networking Activates Recombinases [PDF]

Structure, 2006
Recombinases catalyze the DNA strand-exchange reactions of homologous recombination. The regularity of the filaments they form on DNA, though beautiful, does not explain how they work. Functionally important internal interactions now include multiple intermonomer contacts for activation (Galkin et al., 2006).
openaire   +1 more source

Initial characterization of the Pf-Int recombinase from the malaria parasite Plasmodium falciparum.

PLoS ONE, 2012
BackgroundGenetic variation is an essential means of evolution and adaptation in many organisms in response to environmental change. Certain DNA alterations can be carried out by site-specific recombinases (SSRs) that fall into two families: the serine ...
Mehdi Ghorbal, Christine Scheidig-Benatar, Salma Bouizem, Christophe Thomas, Genevieve Paisley, Claire Faltermeier, Melanie Liu, Artur Scherf, Jose-Juan Lopez-Rubio, Deshmukh N Gopaul   +9 more
doaj   +1 more source

A time for promiscuity in a eukaryotic recombinase [PDF]

Journal of Biological Chemistry, 2017
The exchange of DNA strands between broken and intact molecules lies at the heart of fundamental cellular processes ranging from repairing DNA damage by homologous recombination to generation of genetic diversity during sexual reproduction. New work by Lee and colleagues utilizes the DNA curtain method, an elegant single-molecule technique, to ...
openaire   +2 more sources

Direct observation of subunit rotation during DNA strand exchange by serine recombinases

Nature Communications
Serine recombinases are proposed to catalyse site-specific recombination by a unique mechanism called subunit rotation. Cutting and rejoining DNA occurs within an intermediate synaptic complex comprising a recombinase tetramer bound to two DNA sites ...
Gillian M. Cadden, Jan-Gero Schloetel, Grant McKenzie, Martin R. Boocock, Steven W. Magennis, W. Marshall Stark   +5 more
doaj   +1 more source

Orthogonal LoxPsym sites allow multiplexed site-specific recombination in prokaryotic and eukaryotic hosts

Nature Communications
Site-specific recombinases such as the Cre-LoxP system are routinely used for genome engineering in both prokaryotes and eukaryotes. Importantly, recombinases complement the CRISPR-Cas toolbox and provide the additional benefit of high-efficiency DNA ...
Charlotte Cautereels, Jolien Smets, Jonas De Saeger, Lloyd Cool, Yanmei Zhu, Anna Zimmermann, Jan Steensels, Anton Gorkovskiy, Thomas B. Jacobs, Kevin J. Verstrepen   +9 more
doaj   +1 more source

Phage parasites targeting phage homologous recombinases provide antiviral immunity

Nature Communications
Bacteria often carry multiple genes encoding anti-phage defense systems, clustered in defense islands and phage satellites. Various unrelated anti-phage defense systems target phage-encoded homologous recombinases (HRs) through unclear mechanisms.
Gianluca Debiasi-Anders, Cuncun Qiao, Amrita Salim, Na Li, Ignacio Mir-Sanchis   +4 more
doaj   +1 more source

Rad51 Recombinase and Recombination Mediators [PDF]

Journal of Biological Chemistry, 2003
Patrick, Sung, Lumir, Krejci, Stephen, Van Komen, Michael G, Sehorn   +3 more
openaire   +2 more sources

Fourth-generation gene editors: Integration-based genome engineering. [PDF]

Mol Ther Adv
Kincaid AE, Hewitt AW, Kc R.
europepmc   +1 more source

Evidence of transfer of antimicrobial resistance genes from the porcine pathogen <i>Streptococcus suis</i> to human clinical isolates of <i>Streptococcus agalactiae</i> in a major pig-producing region of Spain. [PDF]

One Health
Uruén C, Lavilla MJ, Libante V, Marín CM, Rezusta A, Payot S, Arenas J.   +6 more
europepmc   +1 more source

Structure, function, and applications of two novel phage recombinases from extreme environments. [PDF]

Nucleic Acids Res
Tarrant E, Cormack IG, Hunter CE, Werbowy O, Dorawa S, Wang L, Steen IH, Sandaa RA, Guðmundsdóttir EE, Ketelsen-Striberny B, Kaczorowska AK, Kaczorowski T, Pohl E, Freitag-Pohl S.   +13 more
europepmc   +1 more source