Results 51 to 60 of about 10,769 (161)
The site-specific integration reaction of
Mobile DNA, 2013 Background A large subfamily of serine recombinases contains long polypeptide segments appended to the C-terminal end of the conserved catalytic domain.
Mandali Sridhar +4 more
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Chromosomal manipulation by site-specific recombinases and fluorescent protein-based vectors. [PDF]
PLoS ONE, 2010 Feasibility of chromosomal manipulation in mammalian cells was first reported 15 years ago. Although this technique is useful for precise understanding of gene regulation in the chromosomal context, a limited number of laboratories have used it in actual
Munehiro Uemura +4 more
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Molecular Evolution of the Tre Recombinase [PDF]
Journal of Visualized Experiments, 2008 Here we report the generation of Tre recombinase through directed, molecular evolution. Tre recombinase recognizes a pre-defined target sequence within the LTR sequences of the HIV-1 provirus, resulting in the excision and eradication of the provirus from infected human cells.
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MEIOB targets single-strand DNA and is necessary for meiotic recombination. [PDF]
PLoS Genetics, 2013 Meiotic recombination is a mandatory process for sexual reproduction. We identified a protein specifically implicated in meiotic homologous recombination that we named: meiosis specific with OB domain (MEIOB).
Benoit Souquet +12 more
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BRCA2 stabilises RAD51 and DMC1 nucleoprotein filaments through a conserved interaction mode
Nature CommunicationsBRCA2 is essential for DNA repair by homologous recombination in mitosis and meiosis. It interacts with recombinases RAD51 and DMC1 to facilitate the formation of nucleoprotein filaments on resected DNA ends that catalyse recombination-mediated repair ...
James M. Dunce, Owen R. Davies
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Boolean Recombinase-Based Devices [PDF]
, 2019 This paper relates to a central problem in synthetic biology, which is that of designing Recombinase-based biological devices by matching a functional specification expressed as a Boolean function. This task is challenging as exploring the space of possibilities is typically unfeasible, and therefore many non-trivial design alternatives remain ...
Pérution-Kihli, Guillaume +4 more
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Multistep allelic conversion in mouse pre-implantation embryos by AAV vectors
Scientific ReportsSite-specific recombinases (SSRs) are critical for achieving precise spatiotemporal control of engineered alleles. These enzymes play a key role in facilitating the deletion or inversion of loci flanked by recombination sites, resulting in the activation
Petr Nickl +6 more
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Tyrosine Recombinase Retrotransposons and Transposons
Microbiology Spectrum, 2015 ABSTRACT Retrotransposons carrying tyrosine recombinases (YR) are widespread in eukaryotes. The first described tyrosine recombinase mobile element, DIRS1, is a retroelement from the slime mold Dictyostelium discoideum . The YR elements are bordered by terminal repeats related to their replication via free
Russell T M, Poulter, Margi I, Butler
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Homologous Recombination in Protozoan Parasites and Recombinase Inhibitors
Frontiers in Microbiology, 2017 Homologous recombination (HR) is a DNA double-strand break (DSB) repair pathway that utilizes a homologous template to fully repair the damaged DNA. HR is critical to maintain genome stability and to ensure genetic diversity during meiosis. A specialized
Andrew A. Kelso +7 more
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Single-Molecule Tethered Particle Motion: Stepwise Analyses of Site-Specific DNA Recombination
Micromachines, 2018 Tethered particle motion/microscopy (TPM) is a biophysical tool used to analyze changes in the effective length of a polymer, tethered at one end, under changing conditions.
Hsiu-Fang Fan +2 more
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