Results 31 to 40 of about 12,788 (278)
High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases
Nature Communications, 2019 The development of site-specific recombinases as genome editing tools is limited by the difficulty of altering their DNA sequence specificity. Here the authors present Rec-seq, a method for identifying specificity determinants and off-target substrates ...
Jeffrey L. Bessen +7 more
doaj +1 more source
mBio, 2018 Uropathogenic Escherichia coli (UPEC) is the most common etiologic agent of uncomplicated urinary tract infection (UTI). An important mechanism of gene regulation in UPEC is phase variation that involves inversion of a promoter-containing DNA element via
E. J. Battaglioli +5 more
doaj +1 more source
In vivo and in vitro characterization and application of tyrosine recombinases for metabolic engineering [PDF]
, 2018 Site-specific recombinases are a family of DNA-modifying enzymes that can recognize short specific DNA sequences and drive recombination between them to rearrange DNA fragments which results in excision, integration or inversion.
Liu, Wei
core +3 more sources
Nature Communications, 2019 Homology directed repair of meiotic double-strand breaks functions via recruitment and assembly of strand-exchange proteins called recombinases. Here the authors reveal and characterize a BRCA2 interactor regulating meiotic recombinases that localizes to
Jingjing Zhang +3 more
doaj +1 more source
The Rad52 SSAP superfamily and new insight into homologous recombination
Communications Biology, 2023 Recent structures of DNA-bound bacterial and phage recombinases provide insights into homologous recombination and suggest relation to the eukaryotic Rad52 and identification of a Rad52 single strand annealing protein (SSAP) superfamily.
Ali Al-Fatlawi +2 more
doaj +1 more source
In silico data analyses of recombinases GdDMC1A and GdDMC1B from Giardia duodenalis
Data in Brief, 2016 Giardia duodenalis is a worldwide protozoa known causing diarrhea in all vertebrates, humans among these. Homologous recombination is a mechanism that provides genomic stability. Two putative recombinases were identified in G.
Ana Laura Torres-Huerta +3 more
doaj +1 more source
Scientific Reports, 2020 The tyrosine-type site-specific DNA recombinase Cre recombines its target site, loxP, with high activity and specificity without cross-recombining the target sites of highly related recombinases. Understanding how Cre achieves this precision is key to be
Anjali Soni +3 more
doaj +1 more source
Promoter Orientation of Prokaryotic Phase-variable Genes by PCR
Bio-Protocol, 2012 One major mechanism of phase variable gene expression in prokaryotes is through inversion of the promoter element for a gene or operon. This protocol describes how to detect the promoter orientation of a phase-variable gene by PCR.
Stacey Bateman, Patrick Seed
doaj +1 more source
Engineering Biology, 2020 Inducible genetic switches based on tyrosine recombinase-based DNA excision are a promising platform for the regulation and control of chimeric antigen receptor (CAR) T cell activity in cancer immunotherapy. These switches exploit the increased stability
Jack E. Bowyer +3 more
doaj +1 more source
Nature Communications, 2021 RAD51 and DMC1 recombinases catalyse high-fidelity and mismatch tolerant recombination, processes that are indispensable for the maintenance of genomic integrity.
Shih-Chi Luo +12 more
doaj +1 more source