Results 31 to 40 of about 10,769 (161)

Promoter Orientation of Prokaryotic Phase-variable Genes by PCR

Bio-Protocol, 2012
One major mechanism of phase variable gene expression in prokaryotes is through inversion of the promoter element for a gene or operon. This protocol describes how to detect the promoter orientation of a phase-variable gene by PCR.
Stacey Bateman, Patrick Seed
doaj   +1 more source

Nearest-neighbor amino acids of specificity-determining residues influence the activity of engineered Cre-type recombinases

Scientific Reports, 2020
The tyrosine-type site-specific DNA recombinase Cre recombines its target site, loxP, with high activity and specificity without cross-recombining the target sites of highly related recombinases. Understanding how Cre achieves this precision is key to be
Anjali Soni, Martina Augsburg, Frank Buchholz, M. Teresa Pisabarro   +3 more
doaj   +1 more source

In silico data analyses of recombinases GdDMC1A and GdDMC1B from Giardia duodenalis

Data in Brief, 2016
Giardia duodenalis is a worldwide protozoa known causing diarrhea in all vertebrates, humans among these. Homologous recombination is a mechanism that provides genomic stability. Two putative recombinases were identified in G.
Ana Laura Torres-Huerta, Rosa María Martínez-Miguel, María Luisa Bazán-Tejeda, Rosa María Bermúdez-Cruz   +3 more
doaj   +1 more source

Mechanistic modelling of tyrosine recombination reveals key parameters determining the performance of a CAR T cell switching circuit

Engineering Biology, 2020
Inducible genetic switches based on tyrosine recombinase-based DNA excision are a promising platform for the regulation and control of chimeric antigen receptor (CAR) T cell activity in cancer immunotherapy. These switches exploit the increased stability
Jack E. Bowyer, Deboki Chakravarti, Wilson W. Wong, Declan G. Bates   +3 more
doaj   +1 more source

Structures of RecBCD in complex with phage-encoded inhibitor proteins reveal distinctive strategies for evasion of a bacterial immunity hub

eLife, 2022
Following infection of bacterial cells, bacteriophage modulate double-stranded DNA break repair pathways to protect themselves from host immunity systems and prioritise their own recombinases.
Martin Wilkinson, Oliver J Wilkinson, Connie Feyerherm, Emma E Fletcher, Dale B Wigley, Mark S Dillingham   +5 more
doaj   +1 more source

Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures

Nature Communications, 2021
RAD51 and DMC1 recombinases catalyse high-fidelity and mismatch tolerant recombination, processes that are indispensable for the maintenance of genomic integrity.
Shih-Chi Luo, Hsin-Yi Yeh, Wei-Hsuan Lan, Yi-Min Wu, Cheng-Han Yang, Hao-Yen Chang, Guan-Chin Su, Chia-Yi Lee, Wen-Jin Wu, Hung-Wen Li, Meng-Chiao Ho, Peter Chi, Ming-Daw Tsai   +12 more
doaj   +1 more source

Multi-color dSTORM microscopy in Hormad1-/- spermatocytes reveals alterations in meiotic recombination intermediates and synaptonemal complex structure.

PLoS Genetics, 2022
Recombinases RAD51 and its meiosis-specific paralog DMC1 accumulate on single-stranded DNA (ssDNA) of programmed DNA double strand breaks (DSBs) in meiosis.
Lieke Koornneef, Johan A Slotman, Esther Sleddens-Linkels, Wiggert A van Cappellen, Marco Barchi, Attila Tóth, Joost Gribnau, Adriaan B Houtsmuller, Willy M Baarends   +8 more
doaj   +1 more source

Characterization of an archaeal recombinase paralog that exhibits novel anti-recombinase activity

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 2020
The process of homologous recombination is heavily dependent on the RecA family of recombinases for repair of DNA double-strand breaks. These recombinases are responsible for identifying homologies and forming heteroduplex DNA between substrate ssDNA and dsDNA templates, activities that are modified by various accessory factors.
Corey, Knadler, Michael, Rolfsmeier, Antonia, Vallejo, Cynthia, Haseltine   +3 more
openaire   +3 more sources

The RAG recombinase: Beyond breaking [PDF]

Mechanisms of Ageing and Development, 2017
DNA double-strand breaks (DSBs) are commonly seen as lesions that threaten genome integrity and contribute to cancer and aging processes. However, in the context of antigen receptor gene assembly, known as V(D)J recombination, DSBs are obligatory intermediates that allow the establishment of genetic diversity and adaptive immunity.
Lescale, Chloé, Deriano, Ludovic
openaire   +3 more sources

Cre Recombinase

Microbiology Spectrum, 2015
ABSTRACT The use of Cre recombinase to carry out conditional mutagenesis of transgenes and insert DNA cassettes into eukaryotic chromosomes is widespread. In addition to the numerous in vivo and in vitro applications that have been reported since Cre was first shown to function in ...
openaire   +2 more sources