Results 51 to 60 of about 520,105 (221)

A rapid and sensitive recombinase aided amplification assay incorporating competitive internal control to detect Bordetella pertussis using the DNA obtained by boiling

International Journal of Infectious Diseases, 2019
Objectives: Pertussis is a highly transmissible acute respiratory infection caused by the bacterial pathogen Bordetella pertussis. The purpose of this study was to develop a rapid, simple and sensitive diagnostic test for detecting this pathogen. Methods:
Rui-qing Zhang, Gui-xia Li, Xin-na Li, Xin-xin Shen, Yuan Gao, Le Wang, Tao Fan, Qing-xia Duan, Ya-kun Wang, Ji Wang, Zhi-shan Feng, Xue-jun Ma   +11 more
doaj   +1 more source

Establishment of a reverse transcription recombinase-aided amplification detection method for porcine group a rotavirus

Frontiers in Veterinary Science, 2022
Porcine rotavirus type A (PoRVA) is the main cause of dehydration and diarrhea in piglets, which has a great impact on the development of the pig industry worldwide. A rapid, accurate and sensitive detection method is conducive to the monitoring, control, and removal of PoRVA.
Yushun Wang, Mincai Nie, Huidan Deng, Siyuan Lai, Yuancheng Zhou, Yuancheng Zhou, Xiangan Sun, Ling Zhu, Ling Zhu, Zhiwen Xu, Zhiwen Xu   +10 more
openaire   +3 more sources

Evaluation of recombinase-based isothermal amplification assays for point-of-need detection of SARS-CoV-2 in resource-limited settings

International Journal of Infectious Diseases, 2022
Objectives: The democratization of diagnostics is one of the key challenges towards containing the transmission of coronavirus disease 2019 (COVID-19) around the globe. The operational complexities of existing PCR-based methods, including sample transfer
Prakash Ghosh, Rajashree Chowdhury, Mohammad Enayet Hossain, Faria Hossain, Mojnu Miah, Md. Utba Rashid, James Baker, Mohammed Ziaur Rahman, Mustafizur Rahman, Xuejun Ma, Malcolm S. Duthie, Ahmed Abd El Wahed, Dinesh Mondal   +12 more
doaj   +1 more source

Detection of low-load Epstein-Barr virus in blood samples by enriched recombinase aided amplification assay

AMB Express, 2022
Key points 1. The RAA with an enrichment step that utilizes magnetic beads coated with M1 protein. 2. A very effective method for detecting low-load virus in blood samples. 3. The first report describing virus detection using this method.
Jing-yi Li, Xiao-ping Chen, Yan-qing Tie, Xiu-li Sun, Rui-qing Zhang, An-na He, Ming-zhu Nie, Guo-hao Fan, Feng-yu Li, Feng-yu Tian, Xin-xin Shen, Zhi-shan Feng, Xue-jun Ma   +12 more
doaj   +1 more source

A Recombinase Aided Amplification Assay for Rapid Detection of the Klebsiella pneumoniae Carbapenemase Gene and Its Characteristics in Klebsiella pneumoniae

Frontiers in Cellular and Infection Microbiology, 2021
Klebsiella pneumoniae carbapenemase genes (blaKPC) play an important role in carbapenem-resistant Enterobacteriaceae in China. A rapid detection method for blaKPC genes and investigations into the molecular characteristics of blaKPC positive Klebsiella ...
Weiwei Zhang, Weiwei Zhang, Yanling Feng, Hanqing Zhao, Chao Yan, Junxia Feng, Lin Gan, Jinghua Cui, Shiyu Liu, Rui Zhang, Shuheng Du, Nannan Li, Wenjian Xu, Juqiang Han, Rongkuan Li, Guanhua Xue, Jing Yuan   +16 more
doaj   +1 more source

Development of a Novel Method for the Clinical Visualization and Rapid Identification of Multidrug-Resistant Candida auris

Microbiology Spectrum, 2023
Outbreaks of multidrug-resistant Candida auris infections, associated with a mortality rate of 30% to 60%, are of serious global concern. Candida auris demonstrates high transmission rates in hospital settings; however, its rapid and accurate ...
X. R. Zhang, T. Ma, Y. C. Wang, S. Hu, Y. Yang   +4 more
doaj   +1 more source

Research Progress of Molecular Biological Technology in Rapid Detection of Foodborne Pathogens

Shipin gongye ke-ji
Foodborne pathogens are one of the important factors affecting food safety. Efficient and rapid detection technology of foodborne pathogens is particularly important to ensure food safety.
Defu ZHANG, Le GAO, Ming ZHANG, Zhixin DU, Xinran LÜ, Xue BAI, Guoqing ZHANG, Xuepeng LI, Jianrong LI   +8 more
doaj   +1 more source

Application and research progress of recombinase isothermal amplification technology in the analysis of animal and plant components

Zhongguo shipin weisheng zazhi
Recently， the analysis of animal- and plant-derived components， mainly based on the adulteration and smuggling of meat products and genetically modified crops， has been a key focus in the field of food safety.
SHI Yunxue, WANG Xin, WANG Jun, YAO Hua, WU Yu   +4 more
doaj   +1 more source

Genomic Characteristics of a Chicken Infectious Anemia Virus in Contaminated Attenuated Vaccine

Frontiers in Veterinary Science, 2022
Chicken infectious anemia virus (CIAV) can be transmitted by contaminated live vaccines, and causes huge economic losses. This study evaluated the contamination status of CIAV in 24 batches of vaccines by recombinase-aided amplification assay (RAA ...
Longfei Chen, Longfei Chen, Longfei Chen, Qi Su, Qi Su, Qi Su, Yan Li, Yan Li, Yan Li, Jinjin Wang, Jinjin Wang, Jinjin Wang, Yawen Zhang, Yawen Zhang, Yawen Zhang, Shuang Chang, Shuang Chang, Shuang Chang, Yixin Wang, Yixin Wang, Yixin Wang, Peng Zhao, Peng Zhao, Peng Zhao   +23 more
doaj   +1 more source

Zinc finger recombinases with adaptable DNA sequence specificity [PDF]

, 2011
Site-specific recombinases have become essential tools in genetics and molecular biology for the precise excision or integration of DNA sequences. However, their utility is currently limited to circumstances where the sites recognized by the recombinase ...
Proudfoot, C., W. Marshall Stark, W Marshall Stark, Proudfoot Chris, Kolb, A.F., Proudfoot, Chris, Arlene L McPherson, Arlene L. McPherson, Kolb Andreas F., McPherson, A.L., McPherson Arlene L., Andreas F Kolb, McPherson, Arlene L, Proudfoot, Christopher, Andreas F. Kolb, Stark W. Marshall, Stark, W.M., Kolb, Andreas F, Stark, W Marshall, Chris Proudfoot   +19 more
core   +1 more source