Results 61 to 70 of about 520,105 (221)

Rapid detection of influenza A viruses using a real-time reverse transcription recombinase-aided amplification assay [PDF]

, 2023
IntroductionInfluenza A viruses (IAVs) are important pathogens of respiratory infections, causing not only seasonal influenza but also influenza pandemics and posing a global threat to public health.
Huan Cui, Jie Pu, Fei Tu, Lei Zhang, Jun Liu, Zhendong Guo, Yunyi Kong, Yuanyuan Sun, Yujie Wei, Zhaoliang Chen, Cheng Zhang, Kui Zhao, Chuncai Liang, Juxiang Liu   +13 more
core   +1 more source

Rapid detection of Ebolavirus using isothermal recombinase‐aided amplification

Journal of Medical Virology
Abstract Ebolavirus disease (EVD) is an often‐lethal disease caused by the genus Ebolavirus (EBOV). Although vaccines are being developed and recently used, outbreak control still relies on a combination of various factors, including rapid identification of EVD cases. This allows rapid patient
Arianna Ceruti, Martin Faye, Moussa M. Diagne, Rea M. Kobialka, Sheila Makiala‐Mandanda, Ousmane Faye, Oumar Faye, Ahmed A. El Wahed, Manfred Weidmann   +8 more
openaire   +2 more sources

Engineering a 660 nm‐Responsive Optogenetic Inducer of Pyroptosis for Precision Cancer Therapy

Advanced Science, EarlyView.
This study reports a bioorthogonal pyroptosis inducer, PyroRACS. By using a red‐light‐responsive Cre‐On genetic switch to control the expression of highly cytotoxic GSDMDNT, PyroRACS enables the artificial induction of pyroptosis with precise spatiotemporal control.
Mengkai Zhang, Zhichao Li, Yonghao Ma, Yangyang Sun, Wei Chen, Weiren Huang   +5 more
wiley   +1 more source

Reverse transcription recombinase polymerase amplification assay for the detection of middle East respiratory syndrome coronavirus [PDF]

, 2013
The emergence of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in the eastern Mediterranean and imported cases to Europe has alerted public health authorities.
El Wahed, Ahmed Abd, Weidmann, Manfred, Wahed, Ahmed Abd El, Hufert, Frank T, Heidenreich, Doris, Abd El Wahed, Ahmed, Patel, Pranav, Hufert, Frank T.   +7 more
core   +1 more source

Clustered Regularly Interspaced Short Palindromic Repeats-Associated Proteins13a combined with magnetic beads, chemiluminescence and reverse transcription-recombinase aided amplification for detection of avian influenza a (H7N9) virus

Frontiers in Bioengineering and Biotechnology, 2023
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and Clustered Regularly Interspaced Short Palindromic Repeats-Associated Proteins (CRISPR-Cas) have promising prospects in the field of nucleic acid molecular diagnostics.
Hongpan Xu, Lijun Peng, Lijun Peng, Jie Wu, Adeel Khan, Yifan Sun, Han Shen, Zhiyang Li   +7 more
doaj   +1 more source

Computation and Structure‐Guided Arginine Scanning Engineers a Hyperactive AP Endonuclease for Multiplex Viral RNA Sensing

Advanced Science, EarlyView.
Computational and structure‐guided arginine scanning rewires the DNA‐binding interface of APE1 to create APE1‐Evo, a hyperactive yet specific AP endonuclease. Integrated into the NAPTUNE‐V2.0 cascade, APE1‐Evo enables amplification‐free, multiplex viral RNA sensing for dengue virus and influenza A/B, highlighting a general strategy for engineering ...
Junlan Wang, Ting Wu, Feizuo Wang, Boyang Wang, Xin Yu Wong, Senfeng Zhang, Shengsheng Ma, Yao Liu, Fangyao Yao, Zhifeng Zeng, Siyuan Yang, Kunming Liu, Qi Wang, Yilong Wang, Yunxiang Zang, Xiaolong Wang, Xingxu Huang, Shao Li, Quanquan Ji, Tao Hu, Chunyi Hu   +20 more
wiley   +1 more source

Development of a Recombinase Polymerase Amplification Assay for Rapid Detection of Francisella noatunensis subsp. orientalis [PDF]

, 2018
Francisella noatunensis subsp. orientalis (Fno) is the causative agent of piscine francisellosis in warm water fish including tilapia. The disease induces chronic granulomatous inflammation with high morbidity and can result in high mortality.
Alexandra Adams (3562352), Lopez-Jimena, Benjamin, Manfred Weidmann (448254), Benjamin Lopez-Jimena (4854436), Jose Gustavo Ramirez-Paredes, Jose Gustavo Ramirez-Paredes (4854439), Manfred Weidmann, Adams, Alexandra, Weidmann, Manfred, Graham Harold (73273), Khalid Shahin, Shahin, Khalid, Harold, Graham, Benjamin Lopez-Jimena, Khalid Shahin (4854433), Alexandra Adams, Ramirez-Paredes, Jose Gustavo, Graham Harold   +17 more
core   +2 more sources

Droplet digital recombinase polymerase amplification (ddRPA) reaction unlocking via picoinjection

, 2022
Recombinase polymerase amplification (RPA) has been recognized as a promising isothermal amplification method for nucleic acid detection. However, the digital format of RPA is still challenging to implement due to its MgOAc-initiated reaction feature and
Cui, Johnson Q., Liu, Frank X., Yao, Shuhuai, Chan, Ka Wai, Leung, Tyler, Park, Hojeong, Tang, Ben Zhong   +6 more
core   +1 more source

Rapid visual detection of dengue virus by combining reverse transcription recombinase-aided amplification with lateral-flow dipstick assay

International Journal of Infectious Diseases, 2020
Objectives: Dengue caused by infection with the dengue virus (DENV) is endemic in the tropical and subtropical regions of the world and of greatest public health concern.
Yufeng Xiong, Yasha Luo, Huan Li, Weixiang Wu, Xiaolin Ruan, Xiaoping Mu   +5 more
doaj   +1 more source

Genetic Ablation and Multi‐Omics Profiling Reveal CEP55 as a Key Driver of Tumorigenesis in Diverse Cancer Models

Advanced Science, EarlyView.
Genetic ablation of Cep55 in Pten‐deficient mouse models delays tumorigenesis. Integrated multi‐omics analyses (proteomics, phosphoproteomics, and spatial transcriptomics) reveal that CEP55 regulates oncogenic signaling (RAS/ERK, PI3K/AKT), integrin/FAK‐mediated adhesion, extracellular matrix (ECM) remodeling, and endocytosis.
Behnam Rashidieh, Yuanhao Yang, Amanda Louise Bain, Phillip Theron, Catharina Vosloo, Sahar Keshvari, Parinaz Ahangar, Brydie Bowden, Simon M. Tria, Ben B. Wang, Sandra Isenmann, Nicholas J. Westra van Holthe, Zherui Xiong, Hani Vu, Sowmya Sharma, Asmerom Sengal, J. Alejandro Lopez, John Finnie, Antonella Papa, Pascal H.G. Duijf, Quan Nguyen, Pirjo M. Apaja, Kum Kum Khanna   +22 more
wiley   +1 more source