Results 31 to 40 of about 5,436 (163)

Rapid Detection and Differentiation of Legionella pneumophila and Non-Legionella pneumophila Species by Using Recombinase Polymerase Amplification Combined With EuNPs-Based Lateral Flow Immunochromatography

Frontiers in Chemistry, 2022
Legionella, a waterborne pathogen, is the main cause of Legionnaires’ disease. Therefore, timely and accurate detection and differentiation of Legionella pneumophila and non-Legionella pneumophila species is crucial. In this study, we develop an easy and
Jungang Du, Biao Ma, Jiali Li, Yaping Wang, Tianyu Dou, Shujuan Xu, Mingzhou Zhang   +6 more
doaj   +1 more source

Isothermal Recombinase Polymerase amplification (RPA) of Schistosoma haematobium DNA and oligochromatographic lateral flow detection [PDF]

Parasites & Vectors, 2015
Accurate diagnosis of urogenital schistosomiasis is vital for surveillance/control programs. Amplification of schistosome DNA in urine by PCR is sensitive and specific but requires infrastructure, financial resources and skilled personnel, often not available in endemic areas.
Rosser, A, Rollinson, D, Forrest, M, Webster, BL   +3 more
openaire   +4 more sources

Amplification Techniques of Recombinase & Polymerase and their Application in Parasite Detection [PDF]

E3S Web of Conferences, 2019
To study the application principles of recombinase polymerase amplification (RPA) and the specific situations of detecting parasites, the principles of RPA are analyzed to find the optimal temperature conditions, advantages, and disadvantages.
Niu Lili, Liu Hongli
doaj   +1 more source

Detection of Neisseria gonorrhoeae and Chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification.

PLoS ONE, 2021
Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are the main pathogenic microorganisms causing sexually transmitted infections. In this study, a multiplex thermostable recombinase polymerase amplification-lateral flow detection (RPA-LFD) assay ...
Jingjing Zhai, Limin Wang, Xiaoliang Qiao, Jianping Zhao, Xuexia Wang, Xiaohong He   +5 more
doaj   +1 more source

Clinical Validation of Two Recombinase-Based Isothermal Amplification Assays (RPA/RAA) for the Rapid Detection of African Swine Fever Virus

Frontiers in Microbiology, 2020
African swine fever (ASF), caused by African swine fever virus (ASFV), is a devastating infectious disease of domestic pigs and wild boars, and has tremendous negative socioeconomic impact on the swine industry and food security worldwide.
Xiaoxu Fan, Lin Li, Yonggang Zhao, Yutian Liu, Chunju Liu, Qinghua Wang, Yaqin Dong, Shujuan Wang, Tianying Chi, Fangfang Song, Chengyou Sun, Yingli Wang, Dengchuriya Ha, Yang Zhao, Yang Zhao, Jingyue Bao, Xiaodong Wu, Zhiliang Wang   +17 more
doaj   +1 more source

Development of a recombinase polymerase amplification (RPA) fluorescence assay for the detection of enterocytozoon hepatopenaei (EHP)

Aquaculture Reports, 2021
The emerging microsporidian parasite Enterocytozoon hepatopenaei (EHP) causes retardation of shrimp growth, leading to significant financial losses in shrimp aquaculture. Therefore, the development of an efficient and sensitive detection method will be conducive to the prevention and control of the shrimp parasite.
Gen Li, Feng Cong, Weiyou Cai, Jinhui Li, Miaoli Wu, Li Xiao, Xiaoliang Hu, Weiwei Zeng, Dongsheng He   +8 more
openaire   +2 more sources

Low-cost recombinase polymerase amplification (RPA) v1

, 2021
This protocol describes the expression of enzymes and creation of a master mix for recombinase polymerase amplification (RPA) assays.
openaire   +1 more source

Establishment and application of dual rpa-basic and rpa-lfd detection method for pasteurella multocida and actinobacillus pleuropneumoniae [PDF]

Kafkas Universitesi Veteriner Fakültesi Dergisi
Based on recombinase polymerase amplification (RPA) detection technology (combined with (lateral flow dipstick, LFD)), it is aimed to establish a dual recombinase polymerase amplification method for the rapid identification of Pasteurella multocida (Pm ...
Jingjing LI, Xiaobing WEI, Shuang LI, Mingcheng LIU, Qianlei ZHU, Kexin WANG, Lei WANG, Yingying CAO, Meinan CHANG, Chunling ZHU, Zhanwei TENG, Xuehan LIU, Huihui ZHANG, Xiaojing XIA, Ke DING   +14 more
doaj   +1 more source

Rapid Detection of Klebsiella pneumoniae in Infants With Severe Infection Disease by Instant Nanofluorescent Microspheres‐Immunochromatography Combined With Recombinase Polymerase Isothermal Amplification Technology [PDF]

J Clin Lab Anal
Schematic illustration of the RPA‐FICTS platform for rapid detection of Klebsiella pneumoniae in infants. Genomic DNA extracted from clinical samples is amplified by recombinase polymerase amplification (RPA) targeting the mdh gene at 37°C, and the biotin‐ and digoxigenin‐labeled amplicons are subsequently detected on a nanofluorescent microsphere ...
Liu Y, Lu Y, Wang X, Yu H, Lu Y, Chen M, Zhu F, Lv X, Wang L, Chen Y.   +9 more
europepmc   +2 more sources

Development and application of recombinase polymerase amplification assay for rapid detection of Blastocystis sp.

Parasitology, 2023
Blastocystis sp. is a common parasite in the intestinal tract of humans and animals. The clinical diagnosis of Blastocystis sp. mainly depends on the microscopic observation of parasite, which can lead to false-negative results.
Xuefang Mei, Changwei Su, Shanrui Zhang, Luwei Jia, Zhenke Yang, Xiaowei Tian, Zhenchao Zhang, Shuai Wang   +7 more
doaj   +1 more source