Results 41 to 50 of about 5,436 (163)

One-Pot RPA-CRISPR/Cas12a Assay With Visual Readout for the Ultra-Specific Detection of Monkeypox Virus Clade I. [PDF]

Microb Biotechnol
This study established a rapid RPA‐CRISPR/Cas12a assay for MPXV clade I detection, achieving an LOD95 of 27.16 copies/μL within 40 min. The assay exhibited no cross‐reactivity with MPXV clade II or other orthopoxviruses. Under simulated conditions, the assay detected more low‐concentration plasmid‐spiked samples than quantitative polymerase chain ...
Li B, Liu L, Jin K, Huang Z, Zhang T, Gao R, Chen H, Niu L, Fan C, Zhang H, Huang P, Wang H.   +11 more
europepmc   +2 more sources

Rapid and Sensitive Recombinase Polymerase Amplification Combined With Lateral Flow Strip for Detecting African Swine Fever Virus

Frontiers in Microbiology, 2019
African swine fever virus (ASFV), the etiological agent of African swine fever (ASF), a hemorrhagic fever of domestic pigs, has devastating consequences for the pig farming industry.
Faming Miao, Jingyuan Zhang, Jingyuan Zhang, Nan Li, Teng Chen, Lidong Wang, Fei Zhang, Lijuan Mi, Jinxia Zhang, Shuchao Wang, Ying Wang, Xintao Zhou, Yanyan Zhang, Min Li, Shoufeng Zhang, Rongliang Hu   +15 more
doaj   +1 more source

A New Duplex Recombinase Polymerase Amplification (D-RPA) Method for the Simultaneous and Rapid Detection of Shigella and Bacillus cereus in Food

Foods, 2023
Shigella and Bacillus cereus are two common foodborne pathogens that cause intestinal diseases and seriously affect human life and health. Traditional microbiological culture methods are time-consuming and laborious, and polymerase chain reaction (PCR ...
Shuna Xiang, Hanyue Zhang, Xiaoyan Cha, Yingting Lin, Ying Shang   +4 more
doaj   +1 more source

Development of a recombinase polymerase amplification assay for rapid detection of Francisella noatunensis subsp. orientalis. [PDF]

PLoS ONE, 2018
Francisella noatunensis subsp. orientalis (Fno) is the causative agent of piscine francisellosis in warm water fish including tilapia. The disease induces chronic granulomatous inflammation with high morbidity and can result in high mortality.
Khalid Shahin, Jose Gustavo Ramirez-Paredes, Graham Harold, Benjamin Lopez-Jimena, Alexandra Adams, Manfred Weidmann   +5 more
doaj   +1 more source

Development and field validation of RPA‐CRISPR‐Cas environmental DNA assays for the detection of brown trout (Salmo trutta) and Arctic char (Salvelinus alpinus)

Environmental DNA, 2023
Molecular methods are rapidly evolving to enable nucleic acid diagnostics outside a laboratory setting. Such techniques are primarily utilizing isothermal amplification such as Recombinase Polymerase Amplification (RPA) and Loop‐Mediated Isothermal ...
Molly Ann Williams, Elvira deEyto, Silke Caestecker, Fiona Regan, Anne Parle‐McDermott   +4 more
doaj   +1 more source

Deoxyuridine triphosphates modified with tyrosine aromatic groups for direct electrochemical detection of double-stranded DNA products of isothermal recombinase polymerase amplification

Electrochemistry Communications, 2021
A set of novel 2′-deoxyuridine-5′-triphosphate (dUTP) derivatives modified with tyrosine (Tyr) or tryptophan (Trp) aromatic groups (m-dUTP) was tested as bearers of electroactive ‘labels’ for double-stranded DNA (dsDNA) detection as well as proper ...
Elena V. Suprun, Svetlana A. Khmeleva, Gulnaz R. Kutdusova, Konstantin G. Ptitsyn, Viktoriya E. Kuznetsova, Sergey A. Lapa, Alexander V. Chudinov, Sergey P. Radko   +7 more
doaj   +1 more source

Application and research progress of recombinase isothermal amplification technology in the analysis of animal and plant components

Zhongguo shipin weisheng zazhi
Recently， the analysis of animal- and plant-derived components， mainly based on the adulteration and smuggling of meat products and genetically modified crops， has been a key focus in the field of food safety.
SHI Yunxue, WANG Xin, WANG Jun, YAO Hua, WU Yu   +4 more
doaj   +1 more source

Translational Barriers and AI‐Driven Challenges of Microfluidics‐Enabled Wearables and Implantable Systems in Personalized Medicine

Advanced Science, EarlyView.
An integrative review of microfluidics‐enabled wearables and implantable systems reveals a single‐track translation pipeline, bridging functional biomaterials with clinical utility. Dynamic feedback loops driven by artificial intelligence advance diagnostics toward personalized closed‐loop theranostics.
Ke Huang, Ching Yin Fong, Xin Huang, Bee Luan Khoo   +3 more
wiley   +1 more source

Coupling Exponential Rolling Circle Amplification to Lateral Flow Devices for Point‐of‐Care Detection of Viral RNA

Angewandte Chemie, Volume 138, Issue 42, 12 October 2026.
A new exponential RCA (E‐RCA) system was developed and coupled to a toehold system for release of a bridging DNA, allowing for lateral flow detection. The E‐RCA method uses a secondary primer (P2) that binds RCA products (RCAP) to generate extended P2 products that can re‐prime a partially self‐complementary circular template, generating a complete ...
Amal Mathai, Monsur Ali, Yingfu Li, John D. Brennan   +3 more
wiley   +2 more sources

Advances in Virus Detection Techniques Based on Recombinant Polymerase Amplification

Molecules
Recombinase polymerase amplification (RPA) has emerged as a rapid, efficient, and highly sensitive method for nucleic acid amplification, thus becoming a focal point of research in the field of virus detection.
Shiwen Wu, Wenhan Yu, Xianshu Fu, Xiaoping Yu, Zihong Ye, Mingzhou Zhang, Yulou Qiu, Biao Ma   +7 more
doaj   +1 more source