Results 21 to 30 of about 5,436 (163)

Point of Care Testing and Recombinase Polymerase Amplification. [PDF]

J Clin Lab Anal
Journal of Clinical Laboratory Analysis, EarlyView.
Weidmann M, El Wahed AA.
europepmc   +2 more sources

Application of a recombinase polymerase amplification (RPA) assay and pilot field testing for Giardia duodenalis at Lake Albert, Uganda. [PDF]

Parasit Vectors, 2020
Abstract Background Giardia duodenalis is a gastrointestinal protozoan causing 184 million cases of giardiasis worldwide annually. Detection is by microscopy or coproantigen assays, although sensitivity is often compromised by intermittent shedding of cysts or trophozoites, or operator expertise.
Molina-Gonzalez SJ, Bhattacharyya T, AlShehri HR, Poulton K, Allen S, Miles MA, Arianitwe M, Tukahebwa EM, Webster B, Russell Stothard J, Bustinduy AL.   +10 more
europepmc   +7 more sources

Rapid isothermal duplex real-time recombinase polymerase amplification (RPA) assay for the diagnosis of equine piroplasmosis. [PDF]

Sci Rep, 2020
AbstractEquine piroplasmosis (EP) is a severe disease of horses caused by the tick-borne protozoa Theileria equi (T. equi) and Babesia caballi (B. caballi). Infectious carriers are not always symptomatic, meaning there is a risk to non-enzootic areas.
Lei R, Wang X, Zhang D, Liu Y, Chen Q, Jiang N.   +5 more
europepmc   +4 more sources

Development and evaluation of fluorescent recombinase polymerase amplification (RPA)-based method for rapid detection of Necator americanus. [PDF]

PLoS Negl Trop Dis
Background Necator americanus is the predominant species causing hookworm infections in humans. Despite advancements in prevention strategies, mild cases of infection still occur, highlighting the need for improved detection technology. Recombinase Polymerase Amplification (RPA) is an isothermal molecular diagnostic known for its sensitivity, speed ...
Liang JR, Yan SN, Yang HY, Yang S, Shen YJ, Huo LL, Cai YC, Mo ZR, Zheng B, Xu B, Hu W.   +10 more
europepmc   +4 more sources

RPA-PCR couple: an approach to expedite plant diagnostics and overcome PCR inhibitors

BioTechniques, 2020
DNA extraction can be lengthy and sometimes ends up with amplification inhibitors. We present the potential of recombinase polymerase amplification (RPA) to replace plant DNA extraction.
Mustafa Ahmad Munawar, Frank Martin, Anna Toljamo, Harri Kokko, Elina Oksanen   +4 more
doaj   +1 more source

Using Cy5-dUTP labelling of RPA-amplicons with downstream microarray analysis for the detection of antibiotic resistance genes

Scientific Reports, 2021
In this report we describe Cy5-dUTP labelling of recombinase-polymerase-amplification (RPA) products directly during the amplification process for the first time.
Christian Warmt, Carolin Kornelia Fenzel, Jörg Henkel, Frank Fabian Bier   +3 more
doaj   +1 more source

Research and Application Progress in Visualized RPA-LFD Nucleic Acid Detection Technology

Shiyan dongwu yu bijiao yixue, 2021
Recombinase polymerase amplification (RPA) is a novel isothermal nucleic acid amplification technology. Compared to PCR, it features ease of use, high efficiency, high sensitivity, high specificity, and does not need specific instruments, which allows it
YU Lingzhi, TAO Lingyun, WEI Xiaofeng
doaj   +1 more source

Data for designing two isothermal amplification assays for the detection of root-infecting fungi on cool-season turfgrasses

Data in Brief, 2018
Loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) are two rapid isothermal amplification methods for detecting three common fungal root pathogens of cool-season turfgrass: Gaeumannomyces avenae, Magnaporthiopsis
Brijesh B. Karakkat, Kurt Hockemeyer, Margot Franchett, Megan Olson, Cortney Mullenberg, Paul L. Koch   +5 more
doaj   +1 more source

Recombinase Polymerase Amplification (RPA) versus PCR for ancient DNA library amplification [PDF]

, 2019
Background : Recombinase Polymerase Amplification (RPA) is a relatively new isothermal methodology for amplifying DNA. RPA is similar to traditional PCR in that it produces an amplicon that is defined by the annealing of two opposing oligonucleotide primers.
Richards, Stephen M, Mitchell, Kieren J, Tobler, Raymond, Cooper, Alan   +3 more
openaire   +1 more source

Recombinase Polymerase Amplification for Rapid Detection of Zoonotic Pathogens: An Overview

Zoonoses, 2022
With the advent of molecular technology, several isothermal techniques for rapid detection of zoonotic pathogens have been developed. Among them, recombinase polymerase amplification (RPA) is becoming an important technology for rapid, sensitive, and ...
Ruichen Lv, Nianhong Lu, Junhu Wang, Yuexi Li, Yong Qi   +4 more
doaj   +1 more source