Endpoint Recombinase Polymerase Amplification (RPA) Assay for Enumeration of Thiocyanate-degrading Bacteria. [PDF]
Microbes Environ, 2022 An endpoint recombination amplification reaction (RPA) assay for assessing the abundance of the gene encoding thiocyanate dehydrogenase (TcDH) in Thiohalobacter has been developed. The RPA reaction was performed at 37°C for 30 min, terminated by the addition of sodium dodecyl sulfate (SDS) solution, and the DNA concentration of the RPA product was ...
Oshiki M +3 more
europepmc +5 more sources
Laboratory Evaluation of a Basic Recombinase Polymerase Amplification (RPA) Assay for Early Detection of Schistosoma japonicum. [PDF]
Pathogens, 2022 Early detection of Schistosoma japonicum (S. japonicum) within its intermediate and definitive hosts is crucial for case finding and disease surveillance, especially in low-endemic areas. Recombinase polymerase amplification (RPA) has many advantages over traditional methods of DNA-amplification, such as polymerase chain reaction (PCR), including high ...
Deng W +7 more
europepmc +5 more sources
Multiplexing using isothermal Recombinase Polymerase Amplification (RPA). [PDF]
BioTechniques, 2017 doaj +2 more sources
Multiplexing using isothermal Recombinase Polymerase Amplification (RPA). [PDF]
BioTechniques, 2018 doaj +2 more sources
Rapid characterization of feline leukemia virus infective stages by a novel nested recombinase polymerase amplification (RPA) and reverse transcriptase-RPA. [PDF]
Sci Rep, 2021 AbstractFeline leukemia virus (FeLV) is a major viral disease in cats, causing leukemia and lymphoma. The molecular detection of FeLV RNA and the DNA provirus are important for staging of the disease. However, the rapid immunochromatographic assay commonly used for antigen detection can only detect viremia at the progressive stage.
Lacharoje S +2 more
europepmc +5 more sources
Development of a recombinase polymerase amplification (RPA) fluorescence assay for the detection of Schistosoma haematobium. [PDF]
Parasit Vectors, 2019 Abstract Background Accurate diagnosis of urogenital schistosomiasis is vital for surveillance and control programmes. While a number of diagnostic techniques are available there is a need for simple, rapid and highly sensitive point-of-need (PON) tests in areas where infection prevalence and intensity are
Rostron P +9 more
europepmc +8 more sources
Establishment of a recombinase polymerase amplification (RPA) assay for the detection of Brucella spp. Infection. [PDF]
Mol Cell Probes, 2019 Brucellosis is a worldwide re-emerging zoonosis. It has an economic impact due to abortion and loss of fertility in livestock. In this study, Real-time recombinase polymerase amplification (RT-RPA-BP26) targeting Brucella spp. bp26 gene and Lateral flow dipstick (LFD-RPA-IS711) combined with SYBR- Green recombinase polymerase amplification (RPA ...
Gumaa MM +7 more
europepmc +3 more sources
Recombinase polymerase amplification (RPA) with lateral flow detection for three Anaplasma species of importance to livestock health. [PDF]
Sci Rep, 2021 AbstractAnaplasma marginale, A. ovis, and A. phagocytophilum are the causative agents of bovine anaplasmosis, ovine anaplasmosis, and granulocytic anaplasmosis, respectively. The gold standard for diagnosis of post-acute and long-term persistent infections is the serological cELISA, which does not discriminate between Anaplasma species and requires ...
Salazar A +3 more
europepmc +6 more sources
Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow Immunoassay for Rapid Detection of Salmonella in Food. [PDF]
Foods, 2019 Salmonella can cause serious foodborne diseases. We have developed a lateral flow immunoassay combined with recombinase polymerase amplification (LFD-RPA) for detection of Salmonella in food. The conserved fragment (fimY) was selected as the target gene. Under an optimal condition (37 °C, 10 min), the sensitivity was 12 colony-forming units (CFU)/mL in
Li J +8 more
europepmc +4 more sources
Recombinase Polymerase Amplification (RPA)-ELISA as an Isothermal Molecular POCT Method for Bacterial Respiratory Infection Diagnosis. [PDF]
Avicenna J Med BiotechnolBackground: Acute Respiratory Infections (ARIs) are a leading cause of childhood mortality worldwide, especially in African and Southeast Asian countries. Point of Care Test (POCT) techniques provide faster diagnoses compared to conventional or real-time PCR methods.
Azizian R +5 more
europepmc +3 more sources