Results 61 to 70 of about 5,436 (163)

Disease control in oilseed rape: Current insights and perspectives for next‐generation integrated pest management

Pest Management Science, EarlyView.
Winter oilseed rape in Europe suffers frequent pathogen losses. This review outlines key integrated pest management (IPM) strategies, rotation, residue and tillage management, optimized sowing, resistance, and targeted fungicides supported by forecasting.
Jon S West, Małgorzata Jędryczka, Nazanin Zamani‐Noor   +2 more
wiley   +1 more source

Single and dual RPA‐CRISPR/Cas assays for point‐of‐need detection of Stewart's wilt pathogen (Pantoea stewartii subsp. stewartii) of corn and Maize dwarf mosaic virus

Pest Management Science, Volume 81, Issue 4, Page 1988-1999, April 2025.
Schematic diagram of the single and dual RPA‐CRISPR/Cas12a/13a diagnostic assays for the detection of Pantoea stewartii subsp. stewartii and Maize dwarf mosaic virus. The validated assays provide a useful and sensitive molecular tool for detecting two quarantine pathogens of maize within a minimal resource framework suitable for fast‐tracking the ...
Qian Tian, Hua Zhou, Zhenxing Zhao, Yongjiang Zhang, Wenjun Zhao, Lulu Cai, Tao Guo   +6 more
wiley   +1 more source

Smartphone-readable RPA-LFA for the high-sensitivity detection of Leishmania kDNA using nanophosphor reporters.

PLoS Neglected Tropical Diseases, 2023
Early diagnosis of infectious diseases improves outcomes by enabling earlier delivery of effective treatment, and helps prevent further transmission by undiagnosed persons.
Adheesha N Danthanarayana, Suman Nandy, Katerina Kourentzi, Binh Vu, Thomas R Shelite, Bruno L Travi, Jakoah Brgoch, Richard C Willson   +7 more
doaj   +1 more source

Microfluidic Molecular Diagnostic Chips: Functional Modules, Integration Strategies, and Emerging Applications

Small Methods, EarlyView.
This comprehensive review delivers an engineering‐centric analysis of microfluidic molecular diagnostic chips, breaking them down into foundational modules from fluid manipulation to bio‐sensing. Beyond chip design, it evaluates sample‐to‐answer integration strategies and commercial readiness levels.
Donghoon Koo, Egor Kokin, Sangyul Baik, Chanyong Park, Minji Kim, Jeongmin Kim, Ryungeun Song, Hosub Lim   +7 more
wiley   +1 more source

Sensitive and rapid detection of Babesia species in dogs by recombinase polymerase amplification with lateral flow dipstick (RPA-LFD)

Scientific Reports, 2022
AbstractCanine babesiosis is a tick-borne disease caused byBabesiaspp., which infects and destroys healthy erythrocytes, leading to mortality and morbidity in dogs. The diagnosis of babesiosis is tedious and time-consuming, especially in latent and chronic infections.
Warunya Onchan, Onchira Ritbamrung, Phanupong Changtor, Waranee Pradit, Siriwadee Chomdej, Korakot Nganvongpanit, Puntita Siengdee, Urasri Suyasunanont, Kittisak Buddhachat   +8 more
openaire   +3 more sources

Recombinase Polymerase Amplification Based Multiplex Lateral Flow Dipstick for Fast Identification of Duck Ingredient in Adulterated Beef

Animals, 2020
Meat adulteration has become a global social problem. In order to protect consumers from meat adulteration, several methods have been developed to identify meat species.
Ming Fu, Quanwang Zhang, Xiang Zhou, Bang Liu   +3 more
doaj   +1 more source

A CRISPR‐Based Rapid Detection Assay for Crayfish Plague (Aphanomyces astaci) From Environmental Samples

Journal of Fish Diseases, EarlyView.
ABSTRACT Crayfish plague, caused by Aphanomyces astaci (Aa), is an infectious disease invasive in Europe, where its rapid spread has resulted in sharp declines of native crayfish species. Monitoring currently relies on a highly sensitive, but costly and time‐consuming qPCR approach.
Christopher Heine, Amadeus Plewnia, David Strand, Simran Gupta, Henrik Krehenwinkel, Gus R. McFarlane   +5 more
wiley   +1 more source

Development and evaluation of a rapid and sensitive RPA assay for specific detection of Vibrio parahaemolyticus in seafood

BMC Microbiology, 2019
Background Vibrio parahaemolyticus (V. parahaemolyticus) is a leading cause of food poisoning and is of great importance to public health due to the frequency and seriousness of the diseases. The simple, timely and efficient detection of this pathogen is
Yunyun Geng, Ke Tan, Libing Liu, Xiao Xia Sun, Baohua Zhao, Jianchang Wang   +5 more
doaj   +1 more source

A novel recombinase polymerase amplification (RPA) assay for the rapid isothermal detection of Neospora caninum in aborted bovine fetuses [PDF]

Veterinary Parasitology, 2018
The development of a method to rapidly diagnose Neospora caninum infection is highly desirable. Recombinase polymerase amplification (RPA), combined with lateral flow (LF) strips, is a novel approach to rapidly amplify and visualize DNA. We have developed a prototype LF-RPA assay, using primers and a probe that targeted a specific sequence in the N ...
Ai-Ling Tian, Hany M. Elsheikha, Dong-Hui Zhou, Yao-Dong Wu, Mu-Xin Chen, Meng Wang, Dan Chen, Xi-Chen Zhang, Xing-Quan Zhu   +8 more
openaire   +2 more sources

The establishment of a recombinase polymerase amplification technique for the detection of mouse poxvirus

BMC Veterinary Research, 2023
Background Ectromelia virus (ECTV) is the causative agent of mousepox in mice. In the past century, ECTV was a serious threat to laboratory mouse colonies worldwide.
Yuexiao Lian, Mengdi Zhang, Yujun Zhu, Miaoli Wu, Bihong Huang, Li Xiao, Kehang Shi, Peide Li, Feng Cong, Huanan Wang   +9 more
doaj   +1 more source