Results 71 to 80 of about 5,436 (163)

Advances in CRISPR‐based detection technologies for severe fever with thrombocytopenia syndrome virus (Dabiebanda virus): A mini review

Clinical and Translational Discovery, Volume 6, Issue 5, October 2026.
This review summarises the detection methods for severe fever with thrombocytopenia syndrome virus based on CRISPR platforms. (A) CRISPR with fluorescent reporter. (B) CRISPR with lateral flow test strip. (C) CRISPR with DNAzyme. (D) Digital CRISPR. (E) CRISPR with single micro‐ring resonator biosensor. (F) self‐amplifying signal amplification strategy
Junhu Wang, Changqiang Zhu, Jinzhao Chu, Yingqing Mao, Yixin Ge, Haiming Yi, Hongming Wang, Yuexi Li, Yong Qi   +8 more
wiley   +1 more source

Synergistic Integration of Recombinase Polymerase Amplification and CRISPR–Cas12a Enables High‐Sensitivity Detection of Mycoplasma bovis

MicrobiologyOpen, Volume 15, Issue 5, October 2026.
This graphical abstract illustrates a rapid diagnostic platform for Mycoplasma bovis detection using Recombinase Polymerase Amplification coupled with CRISPR–Cas12a targeting the uvrC gene. The assay operates at 37°C–39°C, delivers results within 1 h, and demonstrates complete concordance with PCR in cattle and bison clinical samples.
Diba Yaghoubi, Viju V. Pillai
wiley   +1 more source

Protocol for detecting eDNA in ecological rare fish using RPA-CRISPR-Cas12a technology

STAR Protocols
Summary: The recombinase polymerase amplification (RPA)-CRISPR-Cas12a-FQ system enables sensitive detection of environmental DNA (eDNA) in rare fish species.
Xing-Yi Wei, Yang Pei, Li Liu, Péter Hamar, De-Sheng Pei   +4 more
doaj   +1 more source

Machine Learning‐Enhanced Ultrasensitive Immuno‐CRISPR Array Facilitates Early Diagnosis of Alzheimer's Disease by Detecting Multiple Plasma Biomarkers

Advanced Science, Volume 13, Issue 49, 3 September 2026.
This work presents a CRISPR‐based, ultrasensitive multiplex protein detection array capable of simultaneously analyzing six plasma biomarkers associated with Alzheimer's disease (AD). By integrating antibody‐mediated signal transduction, recombinase polymerase amplification, and spatially encoded CRISPR‐Cas12a, the system achieves detection sensitivity
Liding Zhang, Changwen Yang, Qian Yao, Xuewei Du, Shuai Ding, Yaoqiang Shi, Can Sheng, Ming Wang, Ying Han, Haiming Luo   +9 more
wiley   +1 more source

Step-by-Step Development of a Recombinase Polymerase Amplification (RPA) Assay for Sex Identification in Papaya

Applied Biosciences
Papaya is a globally important crop, with production primarily based on hermaphrodite plants. Papaya has three sex types—male, female, and hermaphrodite—determined by flower morphology, but this is only distinguishable at the flowering stage. In this study, a recombinase polymerase amplification (RPA) assay was developed and optimized to identify the ...
José Guadalupe Ávila-Hernández, Alejandro Coreño-Alonso, Mario Alberto Pantoja-Alonso, Francisco Javier Córdoba-Andrade, Rogelio González-González, Corina E. Díaz-Quezada, Alberto Camas-Reyes, Agustino Martínez-Antonio   +7 more
openaire   +3 more sources

Establishment of a recombinase polymerase amplification (RPA) fluorescence assay for the detection of swine acute diarrhea syndrome coronavirus (SADS-CoV). [PDF]

BMC Vet Res, 2022
Cong X, Zhu Y, Liu X, Lian Y, Huang B, Luo Y, Gu Y, Wu M, Shi Y.   +8 more
europepmc   +2 more sources

A Rapid and Simple Detection Assay for Rice Bacterial Leaf Blight by Recombinase Polymerase Amplification

Research in Plant Disease, 2020
Rice bacterial leaf blight (BLB) by Xanthomonas oryzae pv. oryzae (Xoo) is considered to be one of the major rice diseases steadily occurring around the rice-producing countries.
Shinhwa Kim, Bong Choon Lee, Hyun Ju Kim, Soo Yeon Choi, Su Jwa Seo, Sang Min Kim   +5 more
doaj   +1 more source

Strategies and mechanisms of precision genome engineering: From gene editing to genome writing

iMetaOmics, Volume 3, Issue 3, September 2026.
In this review, we examined the progression of genome manipulation from stochastic nuclease‐mediated cutting toward precise editing and programmable genome writing. We discussed tools like multi‐kilobase RNA‐guided integrators and Artificial Intelligence (AI)‐designed effectors and showed how these advances enable researchers to treat genomes as ...
Kerui Huang, Jianhong Tian, Wenyan Zhao, Siqin Zhang, Lixuan Xiang, Meiqing Yang, Lu Liu, Yazhou Huang, Ningyun Zhang, Lei Sun, Peng Xie, Nanyu Kuang, Hong Zhou, Chengfeng Qiu, Jun Yan, Xiaocheng Jiang, Qiang Tu, Yun Wang, Haoliang Hu, Youming Zhang   +19 more
wiley   +1 more source

Integrated one‐pot CRISPR‐Cas detection: Addressing strategies and future prospects

Interdisciplinary Medicine, Volume 4, Issue 5, September 2026.
Integrated one‐pot CRISPR‐Cas detection significantly simplifies workflows, reduces cross‐contamination risks, and shortens turnaround times, making it highly suitable for point‐of‐care testing (POCT) and resource‐limited settings. Key challenges include system compatibility issues between Cas proteins and isothermal amplification, limited multiplexing
Jian Zhou, Xuemei Ren, Yingjie Ren, Ruiping Wu, Ruiman Geng, Le Chen, Zhuo Li   +6 more
wiley   +1 more source

Recombinase Polymerase Amplification assay for detection of the British root-knot nematode, Meloidogyne artiellia

Journal of Nematology
Recombinase polymerase amplification (RPA) is an isothermal in vitro nucleic acid amplification technique that has been adopted for simple, robust, rapid, reliable diagnostics of nematodes.
Subbotin Sergei A., Palomares-Rius Juan E., Castillo Pablo   +2 more
doaj   +1 more source