Results 81 to 90 of about 5,436 (163)

Development of a rapid and visual nucleotide detection method for a Chinese epidemic strain of Orientia tsutsugamushi based on recombinase polymerase amplification assay and lateral flow test

International Journal of Infectious Diseases, 2018
Objectives: Orientia tsutsugamushi is an obligate intracellular pathogen that causes scrub typhus. Diagnosing scrub typhus remains a challenge, and a sensitive, specific, simple, and rapid diagnostic test is still needed.
Yong Qi, Qiong Yin, Yinxiu Shao, Min Cao, Suqin Li, Hongxia Chen, Wanpeng Shen, Jixian Rao, Jiameng Li, Xiaoling Li, Yu Sun, Yu Lin, Yi Deng, Wenwen Zeng, Shulong Zheng, Suyun Liu, Yuexi Li   +16 more
doaj   +1 more source

Reverse Transcription-Recombinase Polymerase (RT-RPA) Amplification with Lateral Flow Detection Short v1

, 2020
The SARS-CoV-2 assay is an isothermal reverse-transcription-recombinase polymerase amplification (RT-RPA) test intended for the presumptive qualitative detection of nucleic acid from the SARS-CoV-2 in oropharyngeal swabs from individuals suspected of Covid-19. Results are for the presumptive detection and identification of SARS-CoV-2 RNA.
Sylvia Daunert, Sapna Deo, Jean-Marc Zingg, Emre Dikici, Yu-Ping Yang, Spencer Owen Seely   +5 more
openaire   +1 more source

An extraction-free and one-pot two-temperature CRISPR/Cas12b system for visual detection of Group B Streptococcus by integrating with RPA

Journal of Clinical Microbiology
Group B Streptococcus (GBS) is a major cause of neonatal infections, and rapid detection is essential for timely clinical intervention. In this study, we developed an extraction-free, one-pot CRISPR/Cas12b assay for visual detection of GBS by combining ...
Chengman Zhao, Ge Li, Chen Shen, Yanjun Xie, Yun Chen, Xiaobo Ying, Yan Chen, Chuanling Zhang   +7 more
doaj   +1 more source

A Rapid and Sensitive CRISPR-Cas12a for the Detection of Legionella pneumophila

Polish Journal of Microbiology
Legionella pneumophila is a common environmental bacterium that can cause severe respiratory disease. In this study, a reliable, rapid, and convenient detection method for L.
LI JIANGHAO, WANG XINYU, WANG XINLING, QU HAI, GAO LIFEI, ZHAO ZHONGLING, LUO PEI, ZHENG YEHUAN   +7 more
doaj   +1 more source

Universal Regulation of DNA Polymerase via Photocaged Primer Enables Light‐Start Isothermal Amplification on Demand

Advanced Science
The development of DNA polymerases with controllable functions, such as hot‐start or light‐start variants, allows on‐demand activation of enzymatic activity and therefore improves reaction specificity. However, the fine control of DNA polymerase activity
Min Qing, Yufan Qin, Zhijin Li, Xiufang Yu, Jianbo Wang, Xiaoqiong Liu, Xin Chen, Chao Yu   +7 more
doaj   +1 more source

Real-Time Monitoring of a Nucleic Acid Amplification Reaction Using a Mass Sensor Based on a Quartz-Crystal Microbalance

Biosensors
Nucleic acid amplification reactions such as polymerase chain reaction (PCR), which uses a DNA polymerase to amplify individual double-stranded DNA fragments, are a useful technique for visualizing the presence of specific genomes.
Hideto Kumagai, Hiroyuki Furusawa
doaj   +1 more source

Isothermal Recombinase Polymerase Amplification (RPA) of E. coli gDNA in Commercially Fabricated PCB-Based Microfluidic Platforms. [PDF]

Micromachines (Basel), 2021
Georgoutsou-Spyridonos M, Filippidou M, Kaprou GD, Mastellos DC, Chatzandroulis S, Tserepi A.   +5 more
europepmc   +1 more source

Development and Validation of a Rapid RPA-PfAgo-Based Meat Species Identification System

Applied Food Research
This study presents a novel detection system combining recombinase polymerase amplification (RPA) with the Argonaute protein from Pyrococcus furiosus (PfAgo) for accurate identification of meat species.
Yaqun Liu, Lianghui Chen, Jialin Wang, Nantao Lin, Kang Zhang, Peikui Yang, Yicun Chen, Chengsong Xie, Min Lin, Zhenxia Zhang, Yuzhong Zheng   +10 more
doaj   +1 more source

A Recombinase Polymerase Amplification Lateral Flow Assay (RPA-LF) for detection of Babesia divergens

Abstract Background: Babesia divergens is a tick-borne apicomplexan parasite found predominantly in Europe, causing babesiosis in livestock and occasionally in humans, with severe disease reported in immunosuppressed or asplenic individuals.
Ernest Chin Jia Bin, Keir Hughes, Cheryl Whitehorn, Ellen knuepfer, Mia White, Kayleigh Hansford, Jolyon Medlock, Taane Clark, Mojca Kristan, Susana campino   +9 more
openaire   +1 more source

A New Rapid Method for the Authentication of Common Octopus (Octopus vulgaris) in Seafood Products Using Recombinase Polymerase Amplification (RPA) and Lateral Flow Assay (LFA). [PDF]

Foods, 2021
Velasco A, Ramilo-Fernández G, Denis F, Oliveira L, Shum P, Silva H, Sotelo CG.   +6 more
europepmc   +1 more source