Results 91 to 100 of about 5,436 (163)

Recombinase Polymerase Amplification (RPA) an Isothermal Technique Suitable for a Range of Applications- Evaluation of RPA Volume Tolerance [PDF]

BioTechniques, 2017
openaire   +5 more sources

Validation of the isothermal Schistosoma haematobium Recombinase Polymerase Amplification (RPA) assay, coupled with simplified sample preparation, for diagnosing female genital schistosomiasis using cervicovaginal lavage and vaginal self-swab samples. [PDF]

PLoS Negl Trop Dis, 2022
Archer J, Patwary FK, Sturt AS, Webb EL, Phiri CR, Mweene T, Hayes RJ, Ayles H, Brienen EAT, van Lieshout L, Webster BL, Bustinduy AL.   +11 more
europepmc   +1 more source

A naked-eye biosensing system based on one-pot RPA-CRISPR/Cas12a driver G4-hemin self-assembly for Mycobacterium tuberculosis

Frontiers in Chemistry
IntroductionThe rapid and accurate identification of Mycobacterium tuberculosis (MTB) is essential for effective tuberculosis (TB) control. However, conventional diagnostic methods for MTB suffer from limitations such as low sensitivity, poor specificity,
Ting Yuan, Ting Yuan, Jianbo Yuan, Jianbo Yuan, Jian Huang, Jian Huang, Nana Li, Nana Li, Huan Cai, Yan Yang, Juan Li, Rui Chen, Xun Min, Xun Min   +13 more
doaj   +1 more source

Bushmeat Species Identification: Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow (LF) Strip for Identification of Formosan Reeves' Muntjac (Muntiacus reevesi micrurus). [PDF]

Animals (Basel), 2021
Hsu YH, Yang WC, Chan KW.
europepmc   +1 more source

Development of a recombinase polymerase amplification (RPA-EXO) and lateral flow assay (RPA-LFA) based on the ITS1 gene for the detection of Angiostrongylus cantonensis in gastropod intermediate hosts. [PDF]

Parasitology, 2021
Jarvi SI, Atkinson ES, Kaluna LM, Snook KA, Steel A.   +4 more
europepmc   +1 more source

Recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) designed for rapid detection of canine distemper virus

Journal of Veterinary Medical Science
In the present study, recombinase polymerase amplification (RPA) was combined with the colloidal gold lateral flow dipstick (LFD) method to establish a new, stable, and efficient assay for the detection of canine distemper virus (CDV). We designed a set of specific primers labeled with biotin and a specific probe labeled with dSpacer and C3 spacer ...
ZHANG, Shanshan, WANG, Chengyu, MENG, Keyin, LIU, Jun   +3 more
openaire   +2 more sources

Isothermal real-time RT-RPA for Machupo virus detection: Field-adaptable sensitivity comparable with laboratory PCR.

PLoS ONE
Isothermal nucleic acid amplification methods, such as recombinase polymerase amplification (RPA), are becoming increasingly vital as diagnostic platforms for neglected tropical diseases by enabling rapid and accurate detection in under-resourced regions.
Marina A Kapitonova, Anna V Shabalina, Igor S Sukhikh, Artemiy A Volkov, Vladimir G Dedkov, Anna S Dolgova   +5 more
doaj   +1 more source

Recombinase polymerase amplification of forensic short tandem repeat loci

Scientific Reports
Short tandem repeats (STRs) are highly polymorphic repetitive DNA sequences extensively used in forensic science for identification of individuals. STR genotyping is usually performed by capillary electrophoresis (CE) or next-generation sequencing (NGS ...
Sonja Škevin, Liesl De Keyzer, Lynn De Waele, Olivier Tytgat, David Van Hoofstat, Dieter Deforce, Filip Van Nieuwerburgh   +6 more
doaj   +1 more source

Point-of-care diagnostic (POCD) method for detecting Bursaphelenchus xylophilus in pinewood using recombinase polymerase amplification (RPA) with the portable optical isothermal device (POID). [PDF]

PLoS One, 2020
Cha D, Kim D, Choi W, Park S, Han H.
europepmc   +1 more source

Development and field evaluation of a rapid detection method for Babesia microti using fluorescent recombinase polymerase amplification(RPA) technology. [PDF]

Sci Rep
Cai Y, Yang HY, Qiang K, Yu C, Yan S, Mo Z, Zheng B, Xu B, Hu W.   +8 more
europepmc   +1 more source