Results 81 to 90 of about 19,653 (194)

From biology to biotechnology: Host‐regulation factors from parasitoid wasps are a source of bioactive molecules with translational potential

Insect Molecular Biology, EarlyView.
Parasitoid wasps deploy maternal and embryonic factors to reprogramme host physiology. Venom, calyx fluid, polydnaviruses, teratocytes and larval secretions act in a coordinated, compartmentalised manner. Host‐regulation factors are promising sources of insecticidal, antimicrobial and bioinspired translational molecules.
Ciro Pedro G. Pinto, Andrew A. Walker, Glenn F. King   +2 more
wiley   +1 more source

A CRISPR‐Based Rapid Detection Assay for Crayfish Plague (Aphanomyces astaci) From Environmental Samples

Journal of Fish Diseases, EarlyView.
ABSTRACT Crayfish plague, caused by Aphanomyces astaci (Aa), is an infectious disease invasive in Europe, where its rapid spread has resulted in sharp declines of native crayfish species. Monitoring currently relies on a highly sensitive, but costly and time‐consuming qPCR approach.
Christopher Heine, Amadeus Plewnia, David Strand, Simran Gupta, Henrik Krehenwinkel, Gus R. McFarlane   +5 more
wiley   +1 more source

Salmonella detection based on fluorescence polarization enhanced by recombinase polymerase amplification

Shipin yu jixie
ObjectiveTo develop a novel fluorescence polarization (FP) detection strategy and achieve highly sensitive Salmonella detection by incorporating recombinase polymerase amplification (RPA) technology.MethodsThe detection mechanism relies on homologous ...
XUE Pengpeng, CHEN Wei, XU Jianguo, TU Jia, QU Wei   +4 more
doaj   +1 more source

Development of a Multiplex Polymerase Chain Reaction Assay for Differentiating Three Lactococcus Species Associated With Piscine Lactococcosis

Journal of Fish Diseases, EarlyView.
ABSTRACT Piscine lactococcosis is an important bacterial disease of farmed fish. The causative agents, Lactococcus garvieae, Lactococcus petauri and Lactococcus formosensis, are closely related, which complicates species‐level identification. We developed a conventional multiplex PCR assay targeting species‐specific genes identified by comparative ...
Tomoaki Yoshino, Tomokazu Takano, Hiroki Tanno, Yukihiro Nakagawa, Yuta Matsuura, Shogo Harakawa, Hidehiro Kondo, Ikuo Hirono, Keiichiro Koiwai, Tomomasa Matsuyama   +9 more
wiley   +1 more source

Anatomical and functional mapping of vagal nociceptive sensory nerve subsets innervating the mouse lower airways by intersectional genetics

The Journal of Physiology, EarlyView.
Abstract figure legend We used an intersectional approach with recombinase‐expressing mice and adeno‐associated virus to map and modulate distinct nociceptive afferents in the vagal ganglia. TRPV1+P2X2+ neurons resided in the nodose ganglion (N), innervated the lungs (many projected into the alveoli) but not the trachea, and projected to the nucleus ...
Mayur J. Patil, Justin Shane Hooper, Seol‐Hee Kim, Parmvir K. Bahia, Nika Pavelkova, Sanjay S. Nair, Teresa S. Darcey, Karina V. Lurye, Meghana Madaram, Jailene Fiallo, Stephen H. Hadley, Thomas E. Taylor‐Clark   +11 more
wiley   +1 more source

Myelin Maintenance and Addition Regulate Synaptic Plasticity in the Adult Mouse Cortex

Glia, Volume 74, Issue 12, December 2026.
We report that existing myelin allows neurons to remain plastic throughout life, while new myelin progressively stabilises synapses along the basal but not apical dendrites of layer V pyramidal neurons in the motor cortex. ABSTRACT Myelin has the capacity to affect synapse number or plasticity across the lifespan.
Renee E. Pepper, Kalina Makowiecki, Kimberley A. Pitman, Raphael Ricci, Angela L. Nicoli, Phuong Tram Nguyen, Catherine A. Blizzard, Ben Emery, Carlie L. Cullen, Kaylene M. Young   +9 more
wiley   +1 more source

A YAP–TEAD–CTCF Transcriptional Regulatory Axis Associated With the Elimination of Injured Hepatocytes From the Liver

Genes to Cells, Volume 31, Issue 6, November 2026.
Injured YAP‐activated hepatocytes are eliminated from the liver. They are extruded toward the sinusoidal lumen, undergo cell death, and are phagocytosed by macrophages. Here, we performed proximity ligation assay, ATAC‐seq, RNA‐seq, and CellChat analyses.
Daiki Hatakeyama, Yoshimi Okamoto‐Uchida, Misaki Kosaka, Haruka Hirose, Satoshi Kofuji, Keiko Danzaki, Yoshiki Endo, Michiko Nakagawa, Akinori Kanai, Yutaka Suzuki, Takeshi Yokoo, Shuji Terai, Teppei Shimamura, Hiroshi Nishina   +13 more
wiley   +1 more source

Advances in CRISPR‐based detection technologies for severe fever with thrombocytopenia syndrome virus (Dabiebanda virus): A mini review

Clinical and Translational Discovery, Volume 6, Issue 5, October 2026.
This review summarises the detection methods for severe fever with thrombocytopenia syndrome virus based on CRISPR platforms. (A) CRISPR with fluorescent reporter. (B) CRISPR with lateral flow test strip. (C) CRISPR with DNAzyme. (D) Digital CRISPR. (E) CRISPR with single micro‐ring resonator biosensor. (F) self‐amplifying signal amplification strategy
Junhu Wang, Changqiang Zhu, Jinzhao Chu, Yingqing Mao, Yixin Ge, Haiming Yi, Hongming Wang, Yuexi Li, Yong Qi   +8 more
wiley   +1 more source

Investigation of the substrate properties of fluorescently labeled pyrimidine triphosphates in recombinase polymerase amplification

Тонкие химические технологии
Objectives. To study the substrate properties of Cy5-labeled deoxynucleoside triphosphates of various natures (dU and dC) in the process of incorporation in the DNA chain during recombinase polymerase amplification (RPA).Methods.
A. S. Epifanov, V. E. Shershov, S. A. Surzhikov, A. V. Chudinov, S. A. Lapa   +4 more
doaj   +1 more source

Synergistic Integration of Recombinase Polymerase Amplification and CRISPR–Cas12a Enables High‐Sensitivity Detection of Mycoplasma bovis

MicrobiologyOpen, Volume 15, Issue 5, October 2026.
This graphical abstract illustrates a rapid diagnostic platform for Mycoplasma bovis detection using Recombinase Polymerase Amplification coupled with CRISPR–Cas12a targeting the uvrC gene. The assay operates at 37°C–39°C, delivers results within 1 h, and demonstrates complete concordance with PCR in cattle and bison clinical samples.
Diba Yaghoubi, Viju V. Pillai
wiley   +1 more source