Results 81 to 90 of about 19,050 (182)

MetF‐Dependent Methionine Biosynthesis Is Required for Mycobacterium tuberculosis Survival and In Vivo Persistence

MicrobiologyOpen, Volume 15, Issue 4, August 2026.
MetF is essential for both in vitro and in vivo growth of Mycobacterium tuberculosis, whereas MetE, previously considered essential in vitro, is dispensable in vivo. Given the importance of methionine and folate metabolism as drug targets, MetF represents a promising candidate, highlighting the need for in vivo validation.
Ikue Tosa, Tomoki Kitahara, Katsuki Takebe, Masaaki Nakayama, Yuko Ito, Akihito Nishiyama, Shintaro Seto, Hotaka Kawai, Hiharu Inoue, Yuki Fukada, Yuki Nishiya, Hitoshi Nagatsuka, Takayuki Wada, Manabu Ato, Sohkichi Matsumoto, Naoya Ohara   +15 more
wiley   +1 more source

SIRT Family: Biological Functions and Therapeutic Targets

MedComm, Volume 7, Issue 8, August 2026.
SIRT1–SIRT7 networks from transgenic mice to human‑relevant therapeutic targets. SIRT1–SIRT7 form an isoform‑, organ‑, and disease‑specific regulatory network. Transgenic Sirt1–7 mouse models define central regulatory SIRTs (SIRT1, SIRT3, SIRT6), context‑dependent modifiers (SIRT2, SIRT4, SIRT5, SIRT7), and their key mechanisms and target organs. These
Jia‐Yi Wang, Feng‐Li Jiang, Fang‐Yuan Zhang, Dong‐Hui Huang, Xiao‐Ying Li, Song Gao, Hua You, Qi‐Jun Wu, Huan‐Huan Chen, Ting‐Ting Gong   +9 more
wiley   +1 more source

A Rapid, Field‐Deployable Diagnostic Platform for Getah Virus Based on RT‐RAA and CRISPR EsCas13d

Microbial Biotechnology, Volume 19, Issue 8, August 2026.
This study developed a rapid, one‐step, visual detection platform termed ROSVD by integrating RT‐RAA with CRISPR–EsCas13d, enabling simplified sample preparation, high‐sensitivity, on‐site detection of GETV within 30 min without the need for sophisticated equipment.
Liu Baoling, Shao Lina, Xu Tong, Jia Qinrui, Zhu Ling, Xu Zhiwen   +5 more
wiley   +1 more source

Endothelial AGGF1 Deficiency Causes Mitochondrial Dysfunction and Contributes to Age‐Elevated Blood Pressure

Aging Cell, Volume 25, Issue 8, August 2026.
Endothelial AGGF1 deficiency contributes to early vascular aging and age‐elevated blood pressure via inhibiting the SESN2/p‐eNOS pathway. Age‐dependent AGGF1 attenuation reduces its binding to the SESN2 promoter. SESN2 downregulation induces mitochondrial respiratory dysfunction and elevated mitochondrial ROS, thereby reducing eNOS phosphorylation ...
Weixin Lv, Xiaojuan Zhong, Qiang Yuan, Xueting Gong, Ya Zhao, Shilin Zhang, Andong Wu, Ming Wan, Xueer Li, Yangyi Zheng, Jiankun Liu, Bingbing Zhou, Yuanzheng Zhu, Limin Zhao, Qiquan Wang, Yang Xiang, Xiao‐Li Tian   +16 more
wiley   +1 more source

Development of PCR, LAMP and HRM‐Based Molecular Species Diagnostic Tools for Identifying Four Agricultural Pests in the Genus Helicoverpa (Lepidoptera: Noctuidae)

Journal of Applied Entomology, Volume 150, Issue 7, Page 900-913, August 2026.
ABSTRACT Within the subfamily Heliothinae, the genus Helicoverpa comprises several highly destructive agricultural pests, including H. armigera, H. assulta, H. punctigera and H. zea. These species cause significant global crop losses due to their wide host range, long‐distance migratory behaviour, and potential for interspecific hybridization.
Minseop Noh, Murtaza Khan, Minyoung Choi, Andreas Zwick, Juil Kim   +4 more
wiley   +1 more source

Salmonella detection based on fluorescence polarization enhanced by recombinase polymerase amplification

Shipin yu jixie
ObjectiveTo develop a novel fluorescence polarization (FP) detection strategy and achieve highly sensitive Salmonella detection by incorporating recombinase polymerase amplification (RPA) technology.MethodsThe detection mechanism relies on homologous ...
XUE Pengpeng, CHEN Wei, XU Jianguo, TU Jia, QU Wei   +4 more
doaj   +1 more source

Chain‐Length Regulation by WzzE Is Necessary for, but Genetically Separable From, Cyclic Enterobacterial Common Antigen Synthesis

Molecular Microbiology, Volume 126, Issue 2, Page 190-207, August 2026.
Here, we demonstrate that polysaccharide chain length regulation plays a distinct role in enabling formation of a cyclic molecule important for the bacterial cell surface. However, this function is necessary, but not sufficient for the cyclic molecule's biosynthesis.
Joseph F. Carr, Yohannes H. Rezenom, Jennifer S. Rudolf, Daniel J. Warzecha, Angela M. Mitchell   +4 more
wiley   +1 more source

Investigation of the substrate properties of fluorescently labeled pyrimidine triphosphates in recombinase polymerase amplification

Тонкие химические технологии
Objectives. To study the substrate properties of Cy5-labeled deoxynucleoside triphosphates of various natures (dU and dC) in the process of incorporation in the DNA chain during recombinase polymerase amplification (RPA).Methods.
A. S. Epifanov, V. E. Shershov, S. A. Surzhikov, A. V. Chudinov, S. A. Lapa   +4 more
doaj   +1 more source

Real-Time Monitoring of a Nucleic Acid Amplification Reaction Using a Mass Sensor Based on a Quartz-Crystal Microbalance

Biosensors
Nucleic acid amplification reactions such as polymerase chain reaction (PCR), which uses a DNA polymerase to amplify individual double-stranded DNA fragments, are a useful technique for visualizing the presence of specific genomes.
Hideto Kumagai, Hiroyuki Furusawa
doaj   +1 more source

Advances in Virus Detection Techniques Based on Recombinant Polymerase Amplification

Molecules
Recombinase polymerase amplification (RPA) has emerged as a rapid, efficient, and highly sensitive method for nucleic acid amplification, thus becoming a focal point of research in the field of virus detection.
Shiwen Wu, Wenhan Yu, Xianshu Fu, Xiaoping Yu, Zihong Ye, Mingzhou Zhang, Yulou Qiu, Biao Ma   +7 more
doaj   +1 more source