Results 51 to 60 of about 36,229 (210)
A recombinase polymerase amplification assay for rapid detection of rabies virus
Scientific Reports, 2021 Rabies is a generally fatal encephalitis caused by a negative-sense single-stranded RNA lyssavirus transmitted to humans mainly from dog bite. Despite the recommendation by WHO and OIE to use the direct immunofluorescence test as standard method ...
Martin Faye +6 more
doaj +1 more source
Chimeric piggyBac transposases for genomic targeting in human cells. [PDF]
, 2012 Integrating vectors such as viruses and transposons insert transgenes semi-randomly and can potentially disrupt or deregulate genes. For these techniques to be of therapeutic value, a method for controlling the precise location of insertion is required ...
Belcaid, Mahdi +9 more
core +5 more sources
Recombinase Polymerase Amplification for Rapid Detection of Zoonotic Pathogens: An Overview
Zoonoses, 2022 With the advent of molecular technology, several isothermal techniques for rapid detection of zoonotic pathogens have been developed. Among them, recombinase polymerase amplification (RPA) is becoming an important technology for rapid, sensitive, and ...
Ruichen Lv +4 more
doaj +1 more source
CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping. [PDF]
, 2017 Broad-scale protein-protein interaction mapping is a major challenge given the cost, time, and sensitivity constraints of existing technologies. Here, we present a massively multiplexed yeast two-hybrid method, CrY2H-seq, which uses a Cre recombinase ...
Bartlett, Anna +12 more
core +2 more sources
Rapid detection of mcr-1 by recombinase polymerase amplification
Journal of Medical Microbiology, 2018 The plasmid-mediated mcr-1 gene conferring colistin resistance has a strong ability to spread. The objective of this study was to establish a rapid and sensitive recombinase polymerase amplification (RPA) method for plasmid-mediated polymyxin-resistant gene mcr-1 detection.Using the reported sequence of the mcr-1 gene, we designed specific primers and ...
Jialiang, Xu +4 more
openaire +2 more sources
Recent developments in nucleic acid identification using solid-phase enzymatic assays [PDF]
, 2014 This review (containing 101 references) covers recent achievements in the development of new approaches for enzymatically assisted detection of nucleic acids on microarrays. We discuss molecular techniques including the polymerase chain reaction, reverse
Ellis, Amanda Vera, Khodakov, Dmitriy A
core +1 more source
Frontiers in Microbiology, 2020 African swine fever (ASF), caused by African swine fever virus (ASFV), is a devastating infectious disease of domestic pigs and wild boars, and has tremendous negative socioeconomic impact on the swine industry and food security worldwide.
Xiaoxu Fan +17 more
doaj +1 more source
Frontiers in Plant Science, 2022 Establishing a portable diagnostic method for identifying plant pathogens is essential to prevent the spread of plant disease, especially in field and customs inspections. Leptosphaeria maculans (L.
Rong Lei +10 more
doaj +1 more source
Blocked recombinase polymerase amplification for mutation analysis of PIK3CA gene [PDF]
Analytical Biochemistry, 2018 A blocked recombinase polymerase amplification (blocked-RPA) approach has been developed for the enrichment of mutated templates in heterogeneous specimens as tumor tissues. This isothermal amplification technique opens alternative solutions for meeting the technological demand of physician office laboratories.
Sara Martorell +3 more
openaire +5 more sources
Research Progress of Molecular Biological Technology in Rapid Detection of Foodborne Pathogens
Shipin gongye ke-jiFoodborne pathogens are one of the important factors affecting food safety. Efficient and rapid detection technology of foodborne pathogens is particularly important to ensure food safety.
Defu ZHANG +8 more
doaj +1 more source