Results 51 to 60 of about 19,653 (194)

Development of recombinase polymerase amplification detection method for Listeria monocytogenes in food

Zhongguo shipin weisheng zazhi, 2021
ObjectiveTo establish a recombinase polymerase amplification (RPA) method for the detection of Listeria monocytogenes (L. monocytogenes) in food.MethodsBased on the hlyA gene of L.
HE Jie, ZHU Chao, HUANG Shanshan, WU Qiaowei, SUN Ping, LAO Huajun   +5 more
doaj   +1 more source

Dual pKa Lipid Nanoparticles for Lung‐tropic mRNA Delivery and pH‐Programmed Endosomal Escape

Advanced Science, EarlyView.
Lipid nanoparticles’ bottleneck lies in low endosomal escape efficiency and liver‐dominant delivery after intravenous administration. To overcome these limitations, we present a stepwise pH‐programmed lipid nanoparticle system enabled by our newly synthesized ionizable lipid.
Seong Gi Lim, Minju Lee, Yiming Wang, Eunjin Choi, Ji Sun Park, Kyungmin Park, Hayoung Jeon, Donghyun Kim, Donghyun Lee, Yeeun Lee, Kee‐Pyo Kim, Eunha Kim, Heebeom Koo   +12 more
wiley   +1 more source

Design of a Novel Diamine Biosensor to Guide the Engineering of Escherichia coli for Cadaverine Overproduction

Advanced Science, EarlyView.
A high‐throughput screening platform was established by integrating an in vivo cadaverine biosensor with a fluorescence‐activated cell sorting (FACS) system. Biosensor‐driven specific exporter tuning, pathway optimization and genome‐wide target mining were conducted to maximize cadaverine production in Escherichia coli.
Jia Feng, Getao Liu, Ding Ma, Jiaxiang Yuan, Xinyi Zhang, Weihao Wang, Chen Ma, Yang Liao, Siyuan Gao, Lei Qin, Jiao Feng, Sheng Xu, Xin Wang, Kequan Chen   +13 more
wiley   +1 more source

RBMS1 Contributes to Early Arteriovenous Fistula Neointimal Remodeling Through Modulation of VSMC Phenotypic Responses

Advanced Science, EarlyView.
Postoperative arteriovenous fistulas exhibit increased RBMS1 in vascular smooth muscle cells (VSMCs). In experimental AVFs, RBMS1 overexpression aggravates, whereas deletion attenuates, neointimal hyperplasia. RBMS1 binds MPZL1 mRNA and promotes exon 5 skipping to generate MPZL1‐Δ5, which interacts with G3BP1 to activate AKT, driving VSMC proliferation,
Zongda Li, Chengwei Zhang, Xinying Yu, Shuang Yao, Yiting Lai, Xiaozhu Sun, Yao Zhao, Xiaoyu Lan, Tingting Hu, Wenjin Li, Zhenzhen Ma, Huimin Ma, Zihan Wang, Haihai Liang, Jundong Jiao   +14 more
wiley   +1 more source

Molecular and serological survey of paratuberculosis in cattle in selected districts of Western Uganda

BMC Veterinary Research, 2022
Knowledge of Mycobacterium avium subsp. paratuberculosis (MAP) herd infection status is important to plan appropriate control and prevention strategies for Paratuberculosis (PTB); however, in Uganda MAP infection status of most herds is unknown.
Judah Ssekitoleko, Lonzy Ojok, Saint Kizito Omala, Mohammed Elwasila Mukhtar, Kamal H. Eltom, El Sagad Eltayeb, Clovice Kankya, Magid Kisekka, Uwe Truyen, Claus-Peter Czerny, Ahmed Abd El Wahed, Julius Boniface Okuni   +11 more
doaj   +1 more source

DNA detection using recombination proteins.

PLoS Biology, 2006
DNA amplification is essential to most nucleic acid testing strategies, but established techniques require sophisticated equipment or complex experimental procedures, and their uptake outside specialised laboratories has been limited. Our novel approach,
Olaf Piepenburg, Colin H Williams, Derek L Stemple, Niall A Armes   +3 more
doaj   +1 more source

Molecular diagnosis of protozoan parasites by Recombinase Polymerase Amplification [PDF]

Acta Tropica, 2018
Infections caused by protozoan parasites affect millions of people around the world. Traditionally, diagnosis was made by microscopy, which is insensitive and in some cases not specific. Molecular methods are highly sensitive and specific, but equipment costs and personnel training limit its availability only to specialized centers, usually far from ...
A, Castellanos-Gonzalez, A C, White, P, Melby, B, Travi   +3 more
openaire   +2 more sources

PrimedRPA: primer design for recombinase polymerase amplification assays [PDF]

Bioinformatics, 2018
Abstract Summary Recombinase polymerase amplification (RPA), an isothermal nucleic acid amplification method, is enhancing our ability to detect a diverse array of pathogens, thereby assisting the diagnosis of infectious diseases and the detection of microorganisms in food and water.
Matthew Higgins, Matt Ravenhall, Daniel Ward, Jody Phelan, Amy Ibrahim, Matthew S. Forrest, Taane G. Clark, Susana G. Campino   +7 more
openaire   +4 more sources

Autonomous Control of Gene Expression Using Endogenous Transcription Signals

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Autonomous cell‐based control of heterologous gene expression can simplify batch‐culture bioprocessing by eliminating external monitoring and extrinsic control of culture conditions. Existing approaches use auto‐induction media, synthetic cell‐cell communication systems, or application‐specific biosensors.
Pakpoom Subsoontorn, Drew Endy, Olivier Borkowski   +2 more
wiley   +1 more source

Strand Invasion Based Amplification (SIBA®): a novel isothermal DNA amplification technology demonstrating high specificity and sensitivity for a single molecule of target analyte.

PLoS ONE, 2014
Isothermal nucleic acid amplification technologies offer significant advantages over polymerase chain reaction (PCR) in that they do not require thermal cycling or sophisticated laboratory equipment.
Mark J Hoser, Hannu K Mansukoski, Scott W Morrical, Kevin E Eboigbodin   +3 more
doaj   +1 more source