Results 31 to 40 of about 36,229 (210)

PrimedRPA: primer design for recombinase polymerase amplification assays [PDF]

open access: yesBioinformatics, 2018
Abstract Summary Recombinase polymerase amplification (RPA), an isothermal nucleic acid amplification method, is enhancing our ability to detect a diverse array of pathogens, thereby assisting the diagnosis of infectious diseases and the detection of microorganisms in food and water.
Matthew Higgins   +7 more
openaire   +3 more sources

Rapid detection of human coronavirus NL63 by isothermal reverse transcription recombinase polymerase amplification

open access: yesJournal of Clinical Virology Plus, 2022
Background: Human coronaviruses are one of the leading causes for respiratory tract infections and for frequent primary care consultation. The human coronavirus NL63 (HCoV..µNL63) is one representative of the seasonal coronaviruses and capable of ...
Aline Dorendorf   +5 more
doaj   +1 more source

A Real-Time Recombinase Polymerase Amplification Method for Rapid Detection of Vibrio vulnificus in Seafood

open access: yesFrontiers in Microbiology, 2020
As an important foodborne pathogen, Vibrio vulnificus gives a significant threat to food safety and public health. Rapid and accurate detection methods for V. vulnificus are required to control its spread.
Xiaohan Yang   +7 more
doaj   +1 more source

Development of a recombinase polymerase amplification (RPA) fluorescence assay for the detection of enterocytozoon hepatopenaei (EHP)

open access: yesAquaculture Reports, 2021
The emerging microsporidian parasite Enterocytozoon hepatopenaei (EHP) causes retardation of shrimp growth, leading to significant financial losses in shrimp aquaculture.
Gen Li   +8 more
doaj   +1 more source

Data for designing two isothermal amplification assays for the detection of root-infecting fungi on cool-season turfgrasses

open access: yesData in Brief, 2018
Loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) are two rapid isothermal amplification methods for detecting three common fungal root pathogens of cool-season turfgrass: Gaeumannomyces avenae, Magnaporthiopsis
Brijesh B. Karakkat   +5 more
doaj   +1 more source

Advancements in the synergy of isothermal amplification and CRISPR-cas technologies for pathogen detection

open access: yesFrontiers in Bioengineering and Biotechnology, 2023
In the realm of pathogen detection, isothermal amplification technology has emerged as a swift, precise, and sensitive alternative to conventional PCR.
Xiaolei Mao   +9 more
doaj   +1 more source

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control [PDF]

open access: yes, 2015
It was recently demonstrated that recombinaseᅠpolymeraseᅠamplification (RPA), an isothermal amplification platform for pathogen detection, may be used to quantify DNA sampleᅠconcentrationᅠusing aᅠstandard curveᅠIn this manuscript, a detailed protocol for
Crannell, Zachary A.   +2 more
core   +1 more source

Rapid detection of Enterococcus and vancomycin resistance using recombinase polymerase amplification [PDF]

open access: yesPeerJ, 2021
Vancomycin-resistant enterococci (VRE), especially Enterococcus faecium, have been a global concern, often causing serious healthcare-associated infections. We established a rapid approach for detecting E.
Pimchanok Panpru   +8 more
doaj   +2 more sources

The burden of congenital Chagas disease and implementation of molecular diagnostic tools in Latin America [PDF]

open access: yes, 2018
It is estimated that between 8000 and 15 000 Trypanosoma cruzi infected babies are born every year to infected mothers in Chagas disease endemic countries. Currently, poor access to and performance of the current diagnostic algorithm, based on microscopy
Cruz, Israel   +7 more
core   +1 more source

Molecular diagnosis of protozoan parasites by Recombinase Polymerase Amplification [PDF]

open access: yesActa Tropica, 2018
Infections caused by protozoan parasites affect millions of people around the world. Traditionally, diagnosis was made by microscopy, which is insensitive and in some cases not specific. Molecular methods are highly sensitive and specific, but equipment costs and personnel training limit its availability only to specialized centers, usually far from ...
A, Castellanos-Gonzalez   +3 more
openaire   +2 more sources

Home - About - Disclaimer - Privacy