Results 31 to 40 of about 19,653 (194)

A recombinase polymerase amplification assay for rapid detection of rabies virus

Scientific Reports, 2021
Rabies is a generally fatal encephalitis caused by a negative-sense single-stranded RNA lyssavirus transmitted to humans mainly from dog bite. Despite the recommendation by WHO and OIE to use the direct immunofluorescence test as standard method ...
Martin Faye, Ahmed Abd El Wahed, Oumar Faye, Jonas Kissenkötter, Bernd Hoffmann, Amadou Alpha Sall, Ousmane Faye   +6 more
doaj   +1 more source

Recombinase Polymerase Amplification for Rapid Detection of Zoonotic Pathogens: An Overview

Zoonoses, 2022
With the advent of molecular technology, several isothermal techniques for rapid detection of zoonotic pathogens have been developed. Among them, recombinase polymerase amplification (RPA) is becoming an important technology for rapid, sensitive, and ...
Ruichen Lv, Nianhong Lu, Junhu Wang, Yuexi Li, Yong Qi   +4 more
doaj   +1 more source

Clinical Validation of Two Recombinase-Based Isothermal Amplification Assays (RPA/RAA) for the Rapid Detection of African Swine Fever Virus

Frontiers in Microbiology, 2020
African swine fever (ASF), caused by African swine fever virus (ASFV), is a devastating infectious disease of domestic pigs and wild boars, and has tremendous negative socioeconomic impact on the swine industry and food security worldwide.
Xiaoxu Fan, Lin Li, Yonggang Zhao, Yutian Liu, Chunju Liu, Qinghua Wang, Yaqin Dong, Shujuan Wang, Tianying Chi, Fangfang Song, Chengyou Sun, Yingli Wang, Dengchuriya Ha, Yang Zhao, Yang Zhao, Jingyue Bao, Xiaodong Wu, Zhiliang Wang   +17 more
doaj   +1 more source

Research Progress of Molecular Biological Technology in Rapid Detection of Foodborne Pathogens

Shipin gongye ke-ji
Foodborne pathogens are one of the important factors affecting food safety. Efficient and rapid detection technology of foodborne pathogens is particularly important to ensure food safety.
Defu ZHANG, Le GAO, Ming ZHANG, Zhixin DU, Xinran LÜ, Xue BAI, Guoqing ZHANG, Xuepeng LI, Jianrong LI   +8 more
doaj   +1 more source

Somatostatin receptor 4 (SSTR4) is a tumor suppressor in cutaneous and head & neck squamous cell carcinomas

Molecular Oncology, EarlyView.
This study identifies somatostatin receptor 4 (Sstr4) as a critical tumor suppressor against skin and head/neck cancers (HNSCC, cSCC, and BCC). The loss of Sstr4 removes a check on cell growth, causing hyperactivation of the MAPK‐ERK signaling pathway (↑).
Ali Taqvi, Tuan Khang Huynh, Adway Kadam, Zahra Kardan, Yue Yan, Amina Jamal Laham, Sampath Kumar Loganathan   +6 more
wiley   +1 more source

A CRISPR/Cas12a-based portable platform for rapid detection of Leptosphaeria maculans in Brassica crops

Frontiers in Plant Science, 2022
Establishing a portable diagnostic method for identifying plant pathogens is essential to prevent the spread of plant disease, especially in field and customs inspections. Leptosphaeria maculans (L.
Rong Lei, Yuan Li, Limei Li, Limei Li, Jingyi Wang, Zhenhai Cui, Rui Ju, Li Jiang, Xiaoling Liao, Pinshan Wu, Xinyi Wang   +10 more
doaj   +1 more source

An Epigenetic Fate Converter Based on Nuclei‐Targeting Lipid Nanoparticles Drives Neuronal Programming of Stem Cells for Spinal Cord Repair

Advanced Functional Materials, EarlyView.
Schematic illustration of LNP‐MPG nuclei‐targeting delivery of HMW‐FGF2 promoting histone acetylation to regulate the fate of DPSCs and treat spinal cord injury. LNPs components include pHMW‐FGF2 plasmid, DSPC, Dlin‐MC3‐DMA, cholesterol, and PEG2000, and are modified with MPG to form HMW‐FGF2@LNP‐MPG (HLM). HLM nuclei‐targets DPSCs to deliver HMW‐FGF2,
Heng Zhou, Qiang Qin, Xiqin Li, Hanyu Ding, Chun Xu, Yan He, Qingsong Ye   +6 more
wiley   +1 more source

Dynamic Operations in Macromolecular Data Storage

Angewandte Chemie, EarlyView.
With the exponential growth of digital data and the limitations of conventional silicon‐based storage and computing technologies, macromolecular data storage, the practice of encoding digital information within large, complex molecules like DNA or synthetic polymers, has emerged as a scalable and sustainable alternative to traditional digital ...
Jakub Ossowski, Oskar Affenberger, Jean‐François Lutz, Maria Nerantzaki   +3 more
wiley   +2 more sources

Recombinase polymerase amplification: method’s characteristics and applications in diagnostics of infectious diseases

Журнал микробиологии, эпидемиологии и иммунобиологии
Isothermal amplification techniques have been actively developed in recent years and are gradually introduced into the range of methods for infectious disease diagnostics. One of the fastest isothermal methods is recombinase polymerase amplification (RPA)
Olga S. Bondareva, Artem A. Baturin, Anna V. Mironova   +2 more
doaj   +1 more source

Systematic Position Mapping of Split CRISPR‐Cas12a Activators Enables Highly Sensitive miRNA Detection and Cancer Cell Stratification

Advanced Science, EarlyView.
ABSTRACT Amplification‐free Cas12a diagnostics with split crRNA enable rapid and programmable target recognition, yet insufficient understanding of DNA activator architecture prevents predictable control over trans‐cleavage activity and sensitivity. Here we systematically map over 200 split DNA activator configurations by introducing nicks at every ...
Xiaoyan Tang, Zhe Li, Yuning Lu, Miao Ma, Zijian Mo, Jiajun Ke, Xinyu Luan, Tiangang Luan, Junqiu Zhai   +8 more
wiley   +1 more source