Results 31 to 40 of about 19,050 (182)

Recombinase Polymerase Amplification for Diagnostic Applications [PDF]

open access: yesClinical Chemistry, 2016
Abstract BACKGROUND First introduced in 2006, recombinase polymerase amplification (RPA) has stirred great interest, as evidenced by 75 publications as of October 2015, with 56 of them just in the last 2 years.
Rana K, Daher   +3 more
openaire   +2 more sources

Detection of Entamoeba histolytica by Recombinase Polymerase Amplification [PDF]

open access: yesThe American Society of Tropical Medicine and Hygiene, 2015
Amebiasis is an important cause of diarrheal disease worldwide and has been associated with childhood malnutrition. Traditional microscopy approaches are neither sensitive nor specific for Entamoeba histolytica. Antigen assays are more specific, but many cases are missed unless tested by molecular methods.
Nair, Gayatri   +8 more
openaire   +3 more sources

An enhanced isothermal amplification assay for viral detection

open access: yesNature Communications, 2020
Current state-of-the-art diagnostics for infectious diseases are sensitive but require extensive equipment. Here the authors develop an enhanced recombinase polymerase amplification reaction for SARS-CoV-2 that allows for inexpensive and rapid testing ...
Jason Qian   +10 more
doaj   +1 more source

P423 Recombinase polymerase amplification for Mucormycosis

open access: yesMedical Mycology, 2022
Abstract Poster session 3, September 23, 2022, 12:30 PM - 1:30 PM   Objectives (1) To develop a Recombinase polymerase amplification (RPA)-based assay to diagnose mucormycosis.
Hallur, Vinaykumar   +4 more
openaire   +1 more source

A recombinase polymerase amplification assay for rapid detection of rabies virus

open access: yesScientific Reports, 2021
Rabies is a generally fatal encephalitis caused by a negative-sense single-stranded RNA lyssavirus transmitted to humans mainly from dog bite. Despite the recommendation by WHO and OIE to use the direct immunofluorescence test as standard method ...
Martin Faye   +6 more
doaj   +1 more source

Recombinase Polymerase Amplification for Rapid Detection of Zoonotic Pathogens: An Overview

open access: yesZoonoses, 2022
With the advent of molecular technology, several isothermal techniques for rapid detection of zoonotic pathogens have been developed. Among them, recombinase polymerase amplification (RPA) is becoming an important technology for rapid, sensitive, and ...
Ruichen Lv   +4 more
doaj   +1 more source

Clinical Validation of Two Recombinase-Based Isothermal Amplification Assays (RPA/RAA) for the Rapid Detection of African Swine Fever Virus

open access: yesFrontiers in Microbiology, 2020
African swine fever (ASF), caused by African swine fever virus (ASFV), is a devastating infectious disease of domestic pigs and wild boars, and has tremendous negative socioeconomic impact on the swine industry and food security worldwide.
Xiaoxu Fan   +17 more
doaj   +1 more source

A CRISPR/Cas12a-based portable platform for rapid detection of Leptosphaeria maculans in Brassica crops

open access: yesFrontiers in Plant Science, 2022
Establishing a portable diagnostic method for identifying plant pathogens is essential to prevent the spread of plant disease, especially in field and customs inspections. Leptosphaeria maculans (L.
Rong Lei   +10 more
doaj   +1 more source

Research Progress of Molecular Biological Technology in Rapid Detection of Foodborne Pathogens

open access: yesShipin gongye ke-ji
Foodborne pathogens are one of the important factors affecting food safety. Efficient and rapid detection technology of foodborne pathogens is particularly important to ensure food safety.
Defu ZHANG   +8 more
doaj   +1 more source

Amplification Techniques of Recombinase & Polymerase and their Application in Parasite Detection [PDF]

open access: yesE3S Web of Conferences, 2019
To study the application principles of recombinase polymerase amplification (RPA) and the specific situations of detecting parasites, the principles of RPA are analyzed to find the optimal temperature conditions, advantages, and disadvantages.
Niu Lili, Liu Hongli
doaj   +1 more source

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