Applying a Linear Amplification Strategy to Recombinase Polymerase Amplification for Uniform DNA Library Amplification [PDF]
ACS Omega, 2019 Recombinase polymerase amplification (RPA) is an isothermal DNA amplification method with broad applications as a point-of-care test and in molecular biology techniques. Currently, most of the applications are focused on target-specific amplification. Because RPA has the advantage of amplifying DNA under isothermal conditions, we utilized RPA as a DNA ...
Jeewon Lee, Sunghoon Heo, Duhee Bang
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Frontiers in Bioengineering and Biotechnology, 2023 The rapid diagnosis of pathogenic infections plays a vital role in disease prevention, control, and public health safety. Recombinase-aided amplification (RAA) is an innovative isothermal nucleic acid amplification technology capable of fast DNA or RNA ...
Xiaoping Li +16 more
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Recombinase Polymerase Amplification for Diagnostic Applications [PDF]
Clinical Chemistry, 2016 Abstract BACKGROUND First introduced in 2006, recombinase polymerase amplification (RPA) has stirred great interest, as evidenced by 75 publications as of October 2015, with 56 of them just in the last 2 years.
Rana K, Daher +3 more
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P423 Recombinase polymerase amplification for Mucormycosis
Medical Mycology, 2022 Abstract Poster session 3, September 23, 2022, 12:30 PM - 1:30 PM Objectives (1) To develop a Recombinase polymerase amplification (RPA)-based assay to diagnose mucormycosis.
Hallur, Vinaykumar +4 more
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Development of a recombinase polymerase amplification lateral flow assay for the detection of active Trypanosoma evansi infections [PDF]
, 2020 Author summary Neglected tropical diseases (NTDs) affecting humans and/or domestic animals severely impair the socio-economic development of endemic areas. One of these diseases, animal trypanosomosis, affects livestock and is caused by the parasites of
Goossens, Julie +5 more
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Development of Recombinase Polymerase Amplification Combined with Lateral Flow Strips for Rapid Detection of Cowpea Mild Mottle Virus [PDF]
The Plant Pathology Journal, 2023 Cowpea mild mottle virus (CPMMV) is a global plant virus that poses a threat to the production and quality of legume crops. Early and accurate diagnosis is essential for effective managing CPMMV outbreaks.
Xinyang Wu +10 more
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Differential DNA accessibility to polymerase enables 30-minute phenotypic β-lactam antibiotic susceptibility testing of carbapenem-resistant Enterobacteriaceae [PDF]
, 2020 The rise in carbapenem-resistant Enterobacteriaceae (CRE) infections has created a global health emergency, underlining the critical need to develop faster diagnostics to treat swiftly and correctly.
Garner, Omai B. +5 more
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Detection of Entamoeba histolytica by Recombinase Polymerase Amplification [PDF]
The American Society of Tropical Medicine and Hygiene, 2015 Amebiasis is an important cause of diarrheal disease worldwide and has been associated with childhood malnutrition. Traditional microscopy approaches are neither sensitive nor specific for Entamoeba histolytica. Antigen assays are more specific, but many cases are missed unless tested by molecular methods.
Nair, Gayatri +8 more
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Veterinary and Animal Science, 2023 Alveolar echinococcosis in slaughtered horses remains a public health issue. This study aimed to develop a Recombinase polymerase amplification (RPA) assay targeting the mitochondrial NADH dehydrogenase subunit 5 (Nad5) gene of Echinococcus ...
Tatsuro Hifumi +5 more
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A recombinase polymerase amplification–based assay for rapid detection of Chlamydia psittaci
Poultry Science, 2021 Chlamydia psittaci is a zoonotic agent of systemic wasting disease in birds and atypical pneumonia in mammalians including humans, constituting a public health risk. A rapid diagnostic assay would be beneficial in screening C. psittaci in the field.
Yanling Pang +6 more
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