Results 11 to 20 of about 36,229 (210)
Recombinase Polymerase Amplification Assay for Rapid Diagnostics of Dengue Infection. [PDF]
PLoS ONE, 2015 Over 2.5 billion people are exposed to the risk of contracting dengue fever (DF). Early diagnosis of DF helps to diminish its burden on public health. Real-time reverse transcription polymerase amplification assays (RT-PCR) are the standard method for ...
Ahmed Abd El Wahed +10 more
doaj +9 more sources
Critical insight into recombinase polymerase amplification technology
Expert Review of Molecular Diagnostics, 2022 Recombinase polymerase amplification (RPA) is a promising and emerging technology for rapidly amplifying target nucleic acid from minimally processed samples and through small portable instruments. RPA is suitable for point-of-care testing (POCT) and on-site field testing, and it is compatible with microfluidic devices.
Mustafa Ahmad Munawar
exaly +7 more sources
Recombinase polymerase amplification in minimally buffered conditions
Biosensors and Bioelectronics, 2022 Application of recombinase polymerase amplification (RPA) for pH-based detection of DNA amplification has been investigated. Commercial RPA kits from TwistDx are modified to minimize their pH buffering capacity. Due to the RPA's unique biochemistry, removal of tris from the amplification kit is not enough to lower the buffering capacity of the RPA ...
Saurabh Tomar +2 more
openaire +4 more sources
Recombinase polymerase amplification: Basics, applications and recent advances
TrAC Trends in Analytical Chemistry, 2018 Recombinase polymerase amplification (RPA) is a highly sensitive and selective isothermal amplification technique, operating at 37-42°C, with minimal sample preparation and capable of amplifying as low as 1-10 DNA target copies in less than 20 min. It has been used to amplify diverse targets, including RNA, miRNA, ssDNA and dsDNA from a wide variety of
Lobato, Ivan Magriñá +1 more
openaire +4 more sources
Frontiers in Cellular and Infection Microbiology, 2022 After the outbreak of SARS-CoV-2, nucleic acid testing quickly entered people’s lives. In addition to the polymerase chain reaction (PCR) which was commonly used in nucleic acid testing, isothermal amplification methods were also important nucleic acid ...
Meiying Tan +5 more
doaj +3 more sources
Recombinase Polymerase Amplification-Based Biosensors for Rapid Zoonoses Screening
International Journal of Nanomedicine, 2023 Xinrui Feng,1,2,* Yan Liu,1,* Yang Zhao,3 Zhe Sun,1,4 Ning Xu,5 Chen Zhao,1 Wei Xia4 1College of Public Health, Jilin Medical University, Jilin, 132013, People’s Republic of China; 2Medical College, Yanbian University, Yanji, 136200, People’s ...
Feng X +6 more
doaj +3 more sources
Rapid detection of hepatitis C virus using recombinase polymerase amplification.
PLoS ONE, 2022 Over 71 million people are infected with hepatitis C virus (HCV) worldwide, and approximately 400,000 global deaths result from complications of untreated chronic HCV.
Catherine T Chia +8 more
doaj +3 more sources
Multiplexing using isothermal Recombinase Polymerase Amplification (RPA). [PDF]
BioTechniques, 2017 doaj +2 more sources
Multiplexing using isothermal Recombinase Polymerase Amplification (RPA). [PDF]
BioTechniques, 2018 doaj +2 more sources
Solvent engineering studies on recombinase polymerase amplification [PDF]
Journal of Bioscience and Bioengineering, 2021 Recombinase polymerase amplification (RPA) is a technique that is used to specifically amplify a target nucleic acid sequence. Unlike the polymerase chain reaction (PCR), RPA is performed at a constant temperature between 37 and 42°C. Therefore, it can be potentially used for the onsite detection of various pathogens when combined with DNA extraction ...
Kenji Kojima +9 more
openaire +2 more sources