Rapid and Sensitive Detection of the Causal Agents of Postharvest Kiwifruit Rot, Botryosphaeria dothidea and Diaporthe eres, Using a Recombinase Polymerase Amplification Assay [PDF]
The Plant Pathology Journal, 2023 The occurrence of postharvest kiwifruit rot has caused great economic losses in major kiwifruit-producing countries. Several pathogens are involved in kiwifruit rot, notably Botryosphaeria dothidea, and Diaporthe species.
Gi-Gyeong Park +2 more
doaj +1 more source
Development of Reverse Transcriptase Recombinase Polymerase Amplification Combined with Lateral Flow Dipstick for Rapid Detection of Tilapia Lake Virus (TiLV): Pilot Study [PDF]
Veterinary SciencesTilapia Lake Virus (TiLV) is well known as a highly contagious viral infection in aquaculture, particularly affecting Tilapia worldwide. Until recently, various TiLV diagnostic methods have been used for rapid and accurate diagnostic procedures that are ...
Saralee Srivorakul +7 more
doaj +2 more sources
Multiplexing using isothermal Recombinase Polymerase Amplification (RPA). [PDF]
BioTechniques, 2017 doaj +2 more sources
Multiplexing using isothermal Recombinase Polymerase Amplification (RPA). [PDF]
BioTechniques, 2018 doaj +2 more sources
Frontiers in Bioengineering and Biotechnology, 2023 The rapid diagnosis of pathogenic infections plays a vital role in disease prevention, control, and public health safety. Recombinase-aided amplification (RAA) is an innovative isothermal nucleic acid amplification technology capable of fast DNA or RNA ...
Xiaoping Li +16 more
doaj +1 more source
Development of Recombinase Polymerase Amplification Combined with Lateral Flow Strips for Rapid Detection of Cowpea Mild Mottle Virus [PDF]
The Plant Pathology Journal, 2023 Cowpea mild mottle virus (CPMMV) is a global plant virus that poses a threat to the production and quality of legume crops. Early and accurate diagnosis is essential for effective managing CPMMV outbreaks.
Xinyang Wu +10 more
doaj +1 more source
Veterinary and Animal Science, 2023 Alveolar echinococcosis in slaughtered horses remains a public health issue. This study aimed to develop a Recombinase polymerase amplification (RPA) assay targeting the mitochondrial NADH dehydrogenase subunit 5 (Nad5) gene of Echinococcus ...
Tatsuro Hifumi +5 more
doaj +1 more source
A recombinase polymerase amplification–based assay for rapid detection of Chlamydia psittaci
Poultry Science, 2021 Chlamydia psittaci is a zoonotic agent of systemic wasting disease in birds and atypical pneumonia in mammalians including humans, constituting a public health risk. A rapid diagnostic assay would be beneficial in screening C. psittaci in the field.
Yanling Pang +6 more
doaj +1 more source
Journal of Clinical Virology Plus, 2022 Background: Human coronaviruses are one of the leading causes for respiratory tract infections and for frequent primary care consultation. The human coronavirus NL63 (HCoV..µNL63) is one representative of the seasonal coronaviruses and capable of ...
Aline Dorendorf +5 more
doaj +1 more source
Solvent engineering studies on recombinase polymerase amplification [PDF]
Journal of Bioscience and Bioengineering, 2021 Recombinase polymerase amplification (RPA) is a technique that is used to specifically amplify a target nucleic acid sequence. Unlike the polymerase chain reaction (PCR), RPA is performed at a constant temperature between 37 and 42°C. Therefore, it can be potentially used for the onsite detection of various pathogens when combined with DNA extraction ...
Kenji Kojima +9 more
openaire +2 more sources