Results 61 to 70 of about 78,833 (285)

DNA detection using recombination proteins.

PLoS Biology, 2006
DNA amplification is essential to most nucleic acid testing strategies, but established techniques require sophisticated equipment or complex experimental procedures, and their uptake outside specialised laboratories has been limited. Our novel approach,
Olaf Piepenburg, Colin H Williams, Derek L Stemple, Niall A Armes   +3 more
doaj   +1 more source

Nucleic Acid Amplification Using Radio Frequency Electrokinetic Heating [PDF]

, 2021
Microfluidic PCR is one of the most common and widely used sample preparation techniques. Most heating methods for microfluidic PCR are heated in one of two ways: (1) the reaction is pumped between different boundary-heating zones, or (2) stationary ...
Yost, Jarad Wilker
core  

Intimate Interaction Between Nucleic Acid and Conjugated Polymers in Organic Electrochemical Transistors Enables Ultrasensitive Biomarker Detection

Advanced Materials, EarlyView.
The intimate interaction between negatively charged RNA molecules and conjugated polymers in organic electrochemical transistors (OECTs) in aqueous electrolytes is investigated. It demonstrates that RNA binding reduces the volumetric capacitance of the polymer channel, enabling the development of an ultrasensitive biosensing platform capable of ...
Hong Liu, Naixiang Wang, Anneng Yang, Jiajun Song, Li Li, Iain McCulloch, Helen Ka‐wai Law, Feng Yan   +7 more
wiley   +1 more source

Cartridge Based Nucleic Acid Amplification Test: The Sherlock of Tuberculosis

, 2019
Emergence of Tuberculosis and Multi drug resistance tuberculosis at higher pace in the population needs rapid diagnosis with accuracy to reduce the transmission of disease and suffering of the patients. The technique of GenexpertMTb/Rif by Cepheid rolled
Sunil Sethi, Mohammad Azhar Khan, Jureka Mankotia   +2 more
core   +1 more source

Global Array-Based Transcriptomics from Minimal Input RNA Utilising an Optimal RNA Isolation Process Combined with SPIA cDNA Probes [PDF]

, 2011
Background: Technical advances in the collection of clinical material, such as laser capture microdissection and cell sorting, provide the advantage of yielding more refined and homogenous populations of cells.
Feuerstein, Giora, Goddard Catharine, Kennedy Laura, Jacqueline Howie, Kennedy, Laura, Giora Feuerstein, Goddard, Catharine; id_orcid, Gino Miele, Struthers Allan, Godfrey Valerie, Lang Chim, Helen Dennis, Mahesh Pauriah, Godfrey, Valerie, Howie, Jacqueline, Chim Lang, Feuerstein Giora, Struthers, Allan, Laura Kennedy, Lang, Chim; id_orcid, Miele Gino, Valerie Godfrey, Dennis Helen, Pauriah, Maheshwar, Crowther Daniel, Miele, Gino, Howie Jacqueline, Crowther, Daniel; id_orcid, Allan Struthers, Daniel Crowther, Dennis, Helen, Pauriah Mahesh, Catharine Goddard   +32 more
core   +1 more source

A Dual‐Bioresponsive and Programmable Microneedle Matrix as a Bioinspired Coupler for Orchestrating Diabetic Bone Regeneration

Advanced Materials, EarlyView.
This project developed a smart bandage‐like patch (a microneedle array) for repairing diabetic bone damage. It intelligently senses signals from infection and inflammation, then releases its medicines in a specific, timed sequence: first an antibacterial agent, then an anti‐inflammatory agent, and finally growth factors.
Yu Wang, Rui Chen, Yu Chen, Haowen Lu, Yuchen Qian, Zhiheng Gao, Zheming Shen, Xiaofei Zheng, Huilin Yang, Jun Zou, Yusen Qiao   +10 more
wiley   +1 more source

Comparison of DNA extraction protocols for Mycobacterium Tuberculosis in diagnosis of tuberculous meningitis by real-time polymerase chain reaction

Journal of Global Infectious Diseases, 2011
Background: Several nucleic acid amplification techniques are available for detection of Mycobacterium tuberculosis (MTB) in pulmonary and extrapulmonary samples, but insufficient data are available on the diagnostic utility of these techniques in ...
Rajeev Thakur, Smita Sarma, Renu Goyal
doaj   +1 more source

Interface‐Engineered Binary Framework Composites: Advancing Porous Materials for Precision Medicine

Advanced Materials Interfaces, EarlyView.
Binary framework composites integrate two complementary porous architectures into a unified platform, enabling multifunctional design, enhanced structural tunability, and improved physicochemical performance. By combining high surface area, ordered porosity, interfacial synergy, and versatile functionalization, these hybrid materials offer new ...
Navid Rabiee, Mohammad Rabiee, Mohammad Akrami‐Hasan‐Kohal, Sidi A. Bencherif   +3 more
wiley   +1 more source

Sensitive and Spatially‐Resolved Electrochemiluminescence via Micropatterning

Advanced Materials Interfaces, EarlyView.
The use of a simple silica nanoparticle surface coating and patterning process can transform ordinary gold electrodes into multiplexed ECL sensing electrodes. Through spatially confined droplet formation and local ECL emission, it is possible to perform multiple analyses simultaneously from individual locations as well as intensify the ECL signal by as
Yuliang Shao, Tatiana Starikova, Mengzhen Xi, Maria Vittoria Balli, Emanuele Luigi Sciuto, Gabriele Giagu, Vincenzo Paratore, Carmelo Corsaro, Enza Fazio, Sabrina Conoci, Luca Prodi, Giovanni Valenti, Pavel A. Levkin   +12 more
wiley   +1 more source

Nucleic acid amplification mediated microbial identification

, 1993
Nucleic acid amplification techniques have and are revolutionising the way research, academia, medicine, environmental and quality control issues are dealt with.
Bruce, Ian J.
core