Establishment of a fluorescent reverse transcription-recombinase polymerase amplification assay for norovirus type GⅡ.4 [PDF]
精准医学杂志Objective To establish a fluorescent reverse transcription-recombinase polymerase amplification (RT-RPA) assay for norovirus (NoV) type GⅡ.4. Methods The NoV GⅡ.4 gene sequence was obtained from NCBI, and the primers and probes were designed by selecting
ZHENG Zhikun, XU Hefei, DING Wei, ZHANG Juan, DU Jiansen, HOU Lin, ZHANG Jin
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Urine Specimens from Pregnant and Nonpregnant Women Inhibitory to Amplification of \u3cem\u3eChlamydia trachomatis\u3c/em\u3e Nucleic Acid by PCR, Ligase Chain Reaction, and Transcription-Mediated Amplification: Identification of Urinary Substances Associated with Inhibition and Removal of Inhibitory Activity [PDF]
, 1998 The presence of endogenous amplification inhibitors in urine may produce false-negative results for the detection of Chlamydia trachomatis nucleic acids by tests such as PCR, ligase chain reaction (LCR), and transcription-mediated amplification (TMA ...
Chernesky, Max A. +7 more
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Bioaffinity detection of pathogens on surfaces [PDF]
, 2010 The demand for improved technologies capable of rapidly detecting pathogens with high sensitivity and selectivity in complex environments continues to be a significant challenge that helps drive the development of new analytical techniques. Surface-based
Faisal, S. N. +4 more
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Advanced Functional Materials, EarlyView.xxxx. ABSTRACT Glioblastoma (GBM) remains one of the most lethal brain cancers, with median survival rarely exceeding 15 months after diagnosis. Interleukin‐12 (IL‐12) is a potent immunostimulatory cytokine capable of reshaping the tumor microenvironment (TME), yet its clinical translation is hindered by systemic toxicity and short half‐life. RNA‐based
Fatima Hameedat +11 more
wiley +1 more source
DNA‐Origami‐Assembled Rhodium Nanoantennas for Deep‐UV Label‐Free Single‐Protein Detection
Advanced Functional Materials, EarlyView.Deep‐UV autofluorescence of single proteins in DNA origami‐based rhodium nanoantennas. Precisely positioned within the plasmonic nanogap, proteins retain their native state while their intrinsic emission is strongly amplified, enabling label‐free single‐molecule detection and opening new opportunities for highly sensitive and specific biosensing ...
Nicco Corduri +8 more
wiley +1 more source
Nanodiamond Quantum Sensors for Probing Free Radical Biology
Advanced Functional Materials, EarlyView.Free radicals play key roles in cellular signaling and disease but remain difficult to measure in living systems. Nanodiamonds (NDs) with nitrogen‐vacancy (NV) centers enable quantum sensing of local magnetic noise via T₁ relaxometry, providing nondestructive radical detection in living cells.
Qi Lu, Yingke Wu, Tanja Weil
wiley +1 more source
Current Trends in RNA Virus Detection via Nucleic Acid Isothermal Amplification-Based Platforms
BiosensorsRibonucleic acid (RNA) viruses are one of the major classes of pathogens that cause human diseases. The conventional method to detect RNA viruses is real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), but it has some ...
Le Thi Nhu Ngoc, Young-Chul Lee
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DNA detection using recombination proteins.
PLoS Biology, 2006 DNA amplification is essential to most nucleic acid testing strategies, but established techniques require sophisticated equipment or complex experimental procedures, and their uptake outside specialised laboratories has been limited. Our novel approach,
Olaf Piepenburg +3 more
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Advanced Healthcare Materials, EarlyView.Neural cell–derived small extracellular vesicles (sEVs) are emerging as pivotal mediators in neurodegenerative diseases, exerting both pathogenic and therapeutic functions. This review synthesizes current evidence on how sEVs from distinct neural cell types regulate neurodegeneration, neuroprotection, biomarker discovery, and targeted drug delivery ...
Muhammad Waqas Salim +4 more
wiley +1 more source
Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits [PDF]
, 2020 The success of fundamental and applied nucleic acid (NA) research depends on NA purity, but obtaining pure NAs from raw, unprocessed samples is challenging.
Ismagilov, Rustem F. +2 more
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