Results 81 to 90 of about 78,833 (285)

Cotton-based nucleic acid extraction

, 2022
: Nucleic acid amplification technique (NAAT)-assisted detection is the primary intervention for pathogen molecular diagnostics. However, NAATs such as quantitative real-time polymerase chain reaction (qPCR) require prior purification or extraction of ...
Souradyuti Ghosh
core   +1 more source

Magnetic Droplet Manipulation on Open Surfaces

Advanced Materials Technologies, EarlyView.
Recent advances in the manipulation of magnetic droplets demonstrate various manipulations on open surfaces, including transport, splitting, merging, and force‐controlled motion, enabled by magnetic particles and external fields. ABSTRACT Manipulation of liquids on a smaller scale enables applications in various fields, particularly diagnostics and ...
Robab Jahangir, Vahid Nasirimarekani
wiley   +1 more source

Characterization and engineering of a DNA polymerase reveals a single amino-acid substitution in the fingers subdomain to increase strand-displacement activity of A-family prokaryotic DNA polymerases

BMC Molecular and Cell Biology, 2019
Background The discovery of thermostable DNA polymerases such as Taq DNA polymerase revolutionized amplification of DNA by polymerase chain reaction methods that rely on thermal cycling for strand separation.
Yvonne Piotrowski, Man Kumari Gurung, Atle Noralf Larsen   +2 more
doaj   +1 more source

Standardization of SARS-CoV-2 Nucleic Acid Amplification Techniques by Calibration and Quantification to the First WHO International Standard for SARS-CoV-2 RNA. [PDF]

Int J Microbiol, 2023
Voermans JJC, Mulders DGJC, Beerkens RJJ, van Duijn M, van der Zwaan L, Rahamat-Langendoen J, van der Eijk A, Koopmans MPG, Molenkamp R.   +8 more
europepmc   +1 more source

Review on Application of Nucleic Acid Amplification Techniques in Pharmaceutical Products Analysis [PDF]

Journal of Applied Pharmaceutical Science, 2015
The aim of this study was to describe application of molecular diagnostic tests based on nucleic acid amplification technologies (NAATs) in pharmaceutical product analysis. NAAT have become widely established in clinical microbiology laboratories in recent years, as well as in quality control (QC) laboratories for food testing, and lately introduced to
openaire   +1 more source

Genome‐Wide CRISPR Screen Identifies a microRNA Orchestrating Pleiotropic Resistance to Targeted Therapy and T Cell Immunity in Melanoma

Advanced Science, EarlyView.
A genome‐wide microRNA CRISPR screen identifies miR‐18a as a master regulator of cross‐resistance in melanoma. Loss of miR‐18a activates the AJUBA–YAP/Hippo axis to confer BRAFi resistance and enhances THBS1–CD47 interaction to impair CD8+ T cell immunity. hnRNP A1 is identified as an upstream regulator of miR‐18a processing.
Zhao Wang, Haotian Liu, Xueqi Wang, Jianjin Teng, Zihan Zheng, Jingya Zhang, Wanyi Xiao, Qian Liang, Jiaxin Li, Xiaomeng Jia, Xin Feng, Hongzhang Cui, Menghan Luo, Tielong Yang, Liuxing Wu, Ke Zhao, Weilong Yang, Mulin Jun Li, Dandan Huang, Jilong Yang   +19 more
wiley   +1 more source

A collaborative study to establish the national standard for SARS-CoV-2 RNA nucleic acid amplification techniques (NAAT) in Taiwan. [PDF]

Biologicals, 2022
Lin PL, Wu MS, Wu PC, Chen HM, Peng YH, Hsu JC, Wang DY.   +6 more
europepmc   +1 more source

RNA Helicase DDX21 Controls CD4+ T Cell Proliferation and Promotes Inflammatory Bowel Disease via Translational Control

Advanced Science, EarlyView.
ABSTRACT Inflammatory bowel disease (IBD) is characterized by dysregulated T cell responses. RNA helicases, including DExD‐box helicase 21 (DDX21), are pivotal in RNA metabolism, but their role in T cell‐mediated pathology during IBD remains unclear. Here, we demonstrate that DDX21 expression in CD4+ T cells correlates with cell cycle and translation ...
Yujuan Zhang, Chen Kan, Xinhui Yang, Yajuan Hao, Xuemin Cai, Mei Yang, Qianqian Zhang, Zhengting Wang, Ning Jiang, Lei Wang, Hua‐Bing Li, Jing Zhou   +11 more
wiley   +1 more source

Lab-on-a-chip systems for the analysis of phytoplankton RNA

, 2013
Monitoring microorganisms in natural water is central to understanding and managing risks to human health and ecosystems. Some phytoplankton can produce toxic blooms which are harmful to aquatic ecosystems and human health.
Bahi, Mahadji
core  

Nucleic Acid Testing of SARS-CoV-2

, 2021
The coronavirus disease 2019 (COVID-19) has caused a large global outbreak. It is accordingly important to develop accurate and rapid diagnostic methods.
Seil Kim, Hee Min Yoo, Il-Hwan Kim
core   +1 more source