Results 91 to 100 of about 78,833 (285)

Multi‐Enzyme Mimetic Molybdenum Nitride Nanozymes Reshape Subgingival Microenvironment for Synergistic Periodontitis Therapy via ROS Regulation and Microbiome Remodeling

Advanced Science, EarlyView.
ABSTRACT Periodontitis, a chronic inflammatory disease initiated and sustained by plaque microorganisms and host immune response, remains an intractable oral disease and a leading cause of tooth loss worldwide. Traditional mechanical debridement and adjunctive antibiotic or antiseptic therapy often shows limited efficacy due to the complex anatomical ...
Weiyu Zhang, Zhongwei Yang, Yunfan Zhang, Longwei Wang, Xiaoyu Zhang, Jiahui Mao, Yizhi Dai, Yifan Yuan, Mingge Wang, Xin Yang, Xin Yu, Jing Liu, Chunying Chen   +12 more
wiley   +1 more source

Cas12a Cis-cleavage mediated lateral flow assay enables multiplex and ultra-specific nucleic acid detection

Nature Communications
CRISPR technology holds significant promise for advancing nucleic acid assays. However, current CRISPR diagnostic techniques, reliant on indiscriminate trans-cleavage mechanisms, face challenges in developing multiplex detection formats.
Mei Lin, Zhiqiang Qiu, Mengen Hao, Weiwei Qi, Ting Zhang, Yuting Shen, Hongrui Xiao, Chaoyue Liang, Longxu Xie, Yongzhong Jiang, Meng Cheng, Tian Tian, Xiaoming Zhou   +12 more
doaj   +1 more source

Rapid and Accurate On-Site Detection of Genetically Modified Maize and Soybean Based on Loop-Mediated Isothermal Amplification and CRISPR/Cas System [PDF]

Shipin Kexue
To address the challenges such as complex operation, long detection time and expensive equipment in nucleic acid amplification-based techniques for the detection of genetically modified (GM) grains, this study developed an efficient and rapid method that
ZHU Yuanyuan, YANG Tianyi, DU Jungang, MA Sihan, CHEN Yanju, LIU Hairong, YE Zunyi, YAN Zhongjun, HUANG Zhibin, YU Zifeng, YANG Qunqing, WU Jian
doaj   +1 more source

Nucleic acid amplification techniques for rapid diagnosis of nontuberculous mycobacteria: A protocol of systematic review and meta-analysis. [PDF]

PLoS One, 2021
Yu G, Shen Y, Xu X, Lin L.
europepmc   +1 more source

3D Chromatin Architecture Provides Insights Into Leaf Trait Variation Among Pear Species

Advanced Science, EarlyView.
ABSTRACT Three‐dimensional (3D) chromatin architecture plays a fundamental role in eukaryotic gene regulations, its functional significance in perennial fruit trees remains poorly characterized despite extensive applications in crop genomics. Here, we developed high‐resolution (∼5 kb) Hi‐C maps of Pyrus and compared 3D genomic architecture of three ...
Yueyuan Liu, Chenhui Han, Cheng Xue, Manyi Sun, Bobo Song, Yongsong Xue, Guangyan Yang, Shaozhuo Xu, Wei Wei, Jiaming Li, Runze Wang, Jun Wu   +11 more
wiley   +1 more source

Rapid Manufacturing of Glass‐Based Digital Nucleic Acid Amplification Chips by Ultrafast Bessel Pulses

Small Science
Advanced nucleic acid amplification techniques require separating test samples into numerous units (digitalization), which enables higher sensitivity and accuracy than traditional methods.
Jiawei Zhang, Kotaro Obata, Kazunari Ozasa, Takanori Uzawa, Yoshihiro Ito, Koji Sugioka   +5 more
doaj   +1 more source

Toward a next-generation diagnostic tool: A review on emerging isothermal nucleic acid amplification techniques for the detection of SARS-CoV-2 and other infectious viruses. [PDF]

Anal Chim Acta, 2022
Islam MM, Koirala D.
europepmc   +1 more source

NUCLEIC ACID AMPLIFICATION AS USED IN THE DIAGNOSIS AND MANAGEMENT OF VIRAL DISEASES: A REVIEW

, 2014
Diagnostic virology is of great importance particularly in disease management and for epidemiological purposes, but is slow and results are obtained when the patient has recovered or succumbed to the infection.
Auwal I. K, Auwal, IK
core   +1 more source

SMAD4 Palmitoylation Drives a Metabolic‐Transcriptional Circuit to Promote Tumorigenesis and Confers Radiosensitivity in Pancreatic Cancer

Advanced Science, EarlyView.
This study identifies palmitoylation as a novel regulatory modification of SMAD4, mediated by ZDHHC22/APT2. It activates fatty acid synthesis, creating a self‐reinforcing SMAD4–FASN–palmitate feedback loop that drives pancreatic cancer growth and enhances radiotherapy sensitivity.
Yang Wang, Shan Zhang, Junping Bai, Xiaobing Li, Yi Han, Min Ji, Chongyi Jiang, Xiaobei Ge, Tianyu Yu, Yongying Hou, Jun Zhang, Wangting Li, Yiran Song, Jiaying Cai, Yingqun Zhou, Liwei An, Feng Wang   +16 more
wiley   +1 more source

The Nucleic Acid Amplification Test in Pulmonary Diseases

Nuclei as a cell nucleus have been found since the 18th century. This discovery was then developed for the need for methods for diagnosing pathogenic organisms of an infectious disease at that time.
Nurwidya, Fariz, Bies, Asri Liqditta, Winardi, Wira, Harinda, Fadlika   +3 more
core   +1 more source