Nature CommunicationsCRISPR technology holds significant promise for advancing nucleic acid assays. However, current CRISPR diagnostic techniques, reliant on indiscriminate trans-cleavage mechanisms, face challenges in developing multiplex detection formats.
Mei Lin +12 more
doaj +1 more source
Nucleic acid amplification techniques for rapid diagnosis of nontuberculous mycobacteria: A protocol of systematic review and meta-analysis. [PDF]
PLoS One, 2021 Yu G, Shen Y, Xu X, Lin L.
europepmc +1 more source
Transcriptomic profiling of 39 commonly-used neuroblastoma cell lines [PDF]
, 2017 Neuroblastoma cell lines are an important and cost-effective model used to study oncogenic drivers of the disease. While many of these cell lines have been previously characterized with SNP, methylation, and/or mRNA expression microarrays, there has not ...
Adams, Rebecca N. +9 more
core +2 more sources
Bioelectrical Interfaces Beyond Excitable Cells: Cancer, Aging, and Gene Expression Modulation
Advanced Materials Interfaces, EarlyView.ABSTRACT The investigation of biological conductivity has evolved from its classical foundation based on ionic fluxes underpinning cardiac and neuronal excitability to a multifaceted regulator of cellular physiology. Traditional approaches for probing electrical events in living matter focused largely on action potentials recording.
Paolo Cadinu +14 more
wiley +1 more source
Small ScienceAdvanced nucleic acid amplification techniques require separating test samples into numerous units (digitalization), which enables higher sensitivity and accuracy than traditional methods.
Jiawei Zhang +5 more
doaj +1 more source
Rapid and Accurate On-Site Detection of Genetically Modified Maize and Soybean Based on Loop-Mediated Isothermal Amplification and CRISPR/Cas System [PDF]
Shipin KexueTo address the challenges such as complex operation, long detection time and expensive equipment in nucleic acid amplification-based techniques for the detection of genetically modified (GM) grains, this study developed an efficient and rapid method that
ZHU Yuanyuan, YANG Tianyi, DU Jungang, MA Sihan, CHEN Yanju, LIU Hairong, YE Zunyi, YAN Zhongjun, HUANG Zhibin, YU Zifeng, YANG Qunqing, WU Jian
doaj +1 more source
Toward a next-generation diagnostic tool: A review on emerging isothermal nucleic acid amplification techniques for the detection of SARS-CoV-2 and other infectious viruses. [PDF]
Anal Chim Acta, 2022 Islam MM, Koirala D.
europepmc +1 more source
Utilization of tmRNA sequences for bacterial identification
, 2001 In recent years, molecular approaches based on nucleotide sequences of ribosomal RNA (rRNA) have become widely used tools for identification of bacteria [1-4].
Amann, R. +4 more
core
Taxonomy of anaerobic digestion microbiome reveals biases associated with the applied high throughput sequencing strategies [PDF]
, 2018 In the past few years, many studies investigated the anaerobic digestion microbiome by means of 16S rRNA amplicon sequencing. Results obtained from these studies were compared to each other without taking into consideration the followed procedure for ...
Angelidaki, Irini +4 more
core +3 more sources
Review on Application of Nucleic Acid Amplification Techniques in Pharmaceutical Products Analysis [PDF]
Journal of Applied Pharmaceutical Science, 2015 The aim of this study was to describe application of molecular diagnostic tests based on nucleic acid amplification technologies (NAATs) in pharmaceutical product analysis. NAAT have become widely established in clinical microbiology laboratories in recent years, as well as in quality control (QC) laboratories for food testing, and lately introduced to
openaire +1 more source