Results 111 to 120 of about 78,833 (285)

Nucleic Acid Diagnostics using Isotachophoresis and Isothermal Amplification

, 2016
Thesis (Ph.D.)--University of Washington, 2016-08Nucleic acid amplification tests (NAAT) have become the gold standard detection technique for many infectious disease diagnoses.
Borysiak, Mark
core  

An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol

, 2012
Background: Mutagenesis plays an essential role in molecular biology and biochemistry. It has also been used in enzymology and protein science to generate proteins which are more tractable for biophysical techniques.
Liu, Huanting, Naismith, James Henderson
core   +1 more source

The BnTFL1‐BnJAM3‐BnSWEETs Module Orchestrates Seed Storage Reserve Accumulation in Brassica napus

Advanced Science, EarlyView.
BnaC03.TFL1 and BnaA01.JAM3 form a complex that facilitates the transport of soluble sugars from the seed coat to the embryo by directly activating the expression of BnSWEETs. Furthermore, BnaC03.TFL1 and BnaA01.JAM3 work cooperatively to promote soluble sugar and oil production while suppressing protein deposition.
Jianjun Wang, Zijin Liu, Minshan Jin, Junting Liu, Shihao Wei, Dengmao Yang, Saiqi Yang, Yuan Guo, Mingxun Chen   +8 more
wiley   +1 more source

The Trichinella Super‐Pangenome Reveals the Evolution of Encapsulation and Predicted Host–Parasite Protein Interactions

Advanced Science, EarlyView.
ABSTRACT The muscle capsule of Trichinella is a critical structure that impedes immune attacks and drug penetration, yet the molecular mechanisms underlying its formation remain poorly understood. Using a high‐quality super‐pangenome comprising 12 Trichinella species, we compared extensive genomic variations between encapsulating and non‐encapsulating ...
Qingbo Lv, Yi Liu, Ning Xu, Xiao Zhang, Yaming Yang, Chengyao Li, Hanhai Mao, Mingyuan Liu, Xiaolei Liu   +8 more
wiley   +1 more source

Agreement between TrueNat Mycobacterium tuberculosis/Rifampicin and Microscopy for Detection of Pulmonary and Extrapulmonary Tuberculosis at a Tertiary Care Hospital of Eastern Uttar Pradesh, India

International Journal of Mycobacteriology
Background: Tuberculosis (TB) remains a significant public health problem in India, with both pulmonary and extra-pulmonary forms contributing substantially to disease burden.
Aroop Mohanty, Kumari Neha Singh, Shweta Singh, Parul Singh, Vivek Hada, Atul Rajaram Rukadikar, Subodh Kumar, Mahima Mittal, Kanishka Kumar, Ruchika Agarwal, Gaurav Gupta, Sudhir Shyam Kushwaha, Rama Shankar Rath   +12 more
doaj   +1 more source

Determining the diet of larvae of western Rock Lobster (Panulirus cygnus) using high-throughput DNA sequencing techniques

, 2012
The Western Australian rock lobster fishery has been both a highly productive and sustainable fishery. However, a recent dramatic and unexplained decline in post-larval recruitment threatens this sustainability.
Waite, A.M., Haruko Takeyama, Peter A Thompson, Lynnath E Beckley, Takeyama, H., Thompson, P.A., Tsai, P., Richard O'Rorke, Anya M Waite, Beckley, L.E., Shane Lavery, Seinen Chow, Andrew G Jeffs, Jeffs, A.G., O'Rorke, R., Lavery, S., Chow, S., Peter Tsai   +17 more
core   +1 more source

Multi‐Omics Profiling Reveals Immunomodulatory and Pro‐Regenerative Effects of a Graphene Oxide–Collagen Scaffold in Massive Rotator Cuff Tears

Advanced Science, EarlyView.
A graphene oxide/collagen scaffold is developed for chronic massive rotator cuff tear repair. The scaffold improves compressive stability, supports reparative mesenchymal differentiation, and modulates the immune microenvironment. In chronic MRCT models, it reduces muscle degeneration, enhances tendon–bone regeneration, and improves functional recovery,
Renwen Wan, Yechuan Deng, Zixin Hu, Yanwei He, Xinting Feng, Jun Ma, Yisheng Chen, Zhijie Zhao, Jie Mei, Zhiheng Lin, Wei Luo, Zhengyuan Fang, Zhufeng Hu, Kunlun Feng, Xinrong Li, Dan Wu, Bowen Han, James Hoi po Hui, Xuanyong Liu, Chen Chen, Gang Chen, Shiyi Chen, Nirong Bao, Jiajun Qiu, Zhiwen Luo   +24 more
wiley   +1 more source

Smart Nanotechnologies for Multimodal Neuromodulation and Brain Interfacing

Advanced Science, EarlyView.
Recent advances in smart nanotechnologies are expanding the toolbox for brain interfacing, from wireless neuromodulation and high‐resolution sensing to targeted delivery within the central nervous system. By combining responsive nanomaterials with bioinspired design, these platforms enable multimodal interactions with neurons and glia, while also ...
Tommaso Curiale, Marie Celine Lefevre, Alessio Carmignani, Maria Cristina Ceccarelli, Matteo Battaglini, Attilio Marino, Gianni Ciofani   +6 more
wiley   +1 more source

Coupling Exponential to Linear Amplification for Endpoint Quantitative Analysis

Advanced Science
Exponential DNA amplification techniques are fundamental in ultrasensitive molecular diagnostics. These systems offer a wide dynamic range, but the quantification requires real‐time monitoring of the amplification reaction.
Coline Kieffer, Yannick Rondelez, Guillaume Gines   +2 more
doaj   +1 more source

Engineering Microbial Particles for Next‐Generation Biomedical Platforms

Advanced Science, EarlyView.
Microbe‐derived particles (MDPs), which include extracellular vesicles, outer membrane vesicles, inclusion bodies, polysaccharide particles, and virus‐like particles, represent a rapidly expanding category of bioinspired nanomaterials. With their natural origin, intrinsic biocompatibility, and highly programmable functionality, MDPs serve as a ...
Yuting Li, Yunjie Lu, Ziyan Huang, Jianjiang Chen, Yanbin Liu, Lei Qin, Chao Wang, Yumin Wu   +7 more
wiley   +1 more source