Results 131 to 140 of about 187,051 (303)

Magnetic Droplet Manipulation on Open Surfaces

Advanced Materials Technologies, EarlyView.
Recent advances in the manipulation of magnetic droplets demonstrate various manipulations on open surfaces, including transport, splitting, merging, and force‐controlled motion, enabled by magnetic particles and external fields. ABSTRACT Manipulation of liquids on a smaller scale enables applications in various fields, particularly diagnostics and ...
Robab Jahangir, Vahid Nasirimarekani
wiley   +1 more source

Agreement between TrueNat Mycobacterium tuberculosis/Rifampicin and Microscopy for Detection of Pulmonary and Extrapulmonary Tuberculosis at a Tertiary Care Hospital of Eastern Uttar Pradesh, India

International Journal of Mycobacteriology
Background: Tuberculosis (TB) remains a significant public health problem in India, with both pulmonary and extra-pulmonary forms contributing substantially to disease burden.
Aroop Mohanty, Kumari Neha Singh, Shweta Singh, Parul Singh, Vivek Hada, Atul Rajaram Rukadikar, Subodh Kumar, Mahima Mittal, Kanishka Kumar, Ruchika Agarwal, Gaurav Gupta, Sudhir Shyam Kushwaha, Rama Shankar Rath   +12 more
doaj   +1 more source

NPM1 Mediates mRNA Sorting into Extracellular Vesicles via Specific RNA Motif Binding and Phase Separation

Advanced Science, EarlyView.
NPM1 selectively sorts specific mRNAs into extracellular vesicles (EVs) by recognizing RNA motifs and forming phase‐separated condensates. These mRNA cargos—including EGFR—are then loaded via multivesicular bodies. This active sorting pathway, validated in EVs derived from both lung cancer cells and patient serum, reveals a specific mechanism for ...
Kaixiang Zhang, Gaoge Sun, Xiangzheng Liu, Shuai Zuo, Pengyuan Wang, Boxiao Zhan, Zhi John Lu, Hang Yin, Ying Zhang   +8 more
wiley   +1 more source

De Novo Design and Directed Evolution Refinement of Mirror‐Image Protein Binders Targeting Interleukin‐4

Advanced Science, EarlyView.
This study presents the de novo design and directed evolution of a mirror‐image D‐protein inhibitor targeting human interleukin‐4 (IL‐4). The engineered molecule exhibits nanomolar binding affinity for IL‐4 and effectively inhibits IL‐4–mediated signaling.
Liqing Xu, Yuxiang Ren, Tongyue Wang, Hongyong Li, Yue Yao, Lei Liu, Ke Sun, Peilong Lu   +7 more
wiley   +1 more source

Coupling Exponential to Linear Amplification for Endpoint Quantitative Analysis

Advanced Science
Exponential DNA amplification techniques are fundamental in ultrasensitive molecular diagnostics. These systems offer a wide dynamic range, but the quantification requires real‐time monitoring of the amplification reaction.
Coline Kieffer, Yannick Rondelez, Guillaume Gines   +2 more
doaj   +1 more source

Combined use of 16S ribosomal DNA and 16S rRNA to study the bacterial community of polychlorinated biphenyl-polluted soil

, 2001
The bacterial diversity assessed from clone libraries prepared from rRNA (two libraries) and ribosomal DNA (rDNA) (one library) from polychlorinated biphenyl (PCB)-polluted soil has been analyzed.
Amann, R., Llobet-Brossa, E., Moore, E., Nogales, B., Rosselló-Mora, R., Timmis, K.   +5 more
core  

An Artificial Antibody‐Based Toolbox Accelerates Validation of Hidden Microproteins Encoded by the Dark Genome

Advanced Science, EarlyView.
Microproteins are hidden treasures encoded by the “dark proteome” but remain largely underexplored due to the lack of highly efficient tools. We developed a molecularly imprinted polymers (MIPs)‐based toolbox (CLAIMID) to achieve accelerated and ultrasensitive microproteins validation at multiple biological scales (single living cells, cell populations,
Hui He, Anqi Zhang, Zhanchen Guo, Qi Li, Song Gao, Wenyan Guan, Ying Li, Jingran Chen, Sijia Sun, Jian He, Zhen Liu   +10 more
wiley   +1 more source

Embedded CRISPRi Enhances Gene‐Silencing Efficiency in Drosophila

Advanced Science, EarlyView.
Current CRISPR interference (CRISPRi) technology in Drosophila has limited efficiency. This study introduces the emCRISPRi platform, which significantly enhances transcriptional silencing efficacy by embedding inhibitory domains within the dCas9 architecture.
Pengchong Fu, Xuedi Zhang, Ying Zhou, Jie Zheng, Angyang Sun, Kailong Zhuang, Weiwei Bao, Guanjun Gao   +7 more
wiley   +1 more source

A Nanoparticle‐Integrated Complete Manufacturing Pipeline of Chemically Engineered Exosomes

Advanced Science, EarlyView.
We report a novel manufacture technology of chemically engineered exosomes. The four steps of manufacturing, i.e., biogenesis, loading, isolation, and storage, are integrated by the use of a nanoparticle. The manufacture technology incorporates three innovative components, i.e., a new nano‐bio effect, a new composite nanoparticle, and a new isolation ...
Xiaowei Wen, Zixing Xu, Zerun Hao, Yanming Chen, Kai Xie, Haofan Yin, Xueying Wang, Jie Min, Sihan Sun, Baiding Chen, Chengxiu Ling, Mingming Xu, Yizhao Chen, Gang Ruan   +13 more
wiley   +1 more source

Enhancing ELISA Sensitivity: From Surface Engineering to Synthetic Biology

Biosensors
Accurate and sensitive detection of protein biomarkers is critical for advancing in vitro diagnostics (IVD), yet conventional enzyme-linked immunosorbent assays (ELISA) often fall short in terms of sensitivity compared to nucleic acid-based tests ...
Hye-Bin Jeon, Dong-Yeon Song, Yu Jin Park, Dong-Myung Kim   +3 more
doaj   +1 more source