Results 121 to 130 of about 78,833 (285)

Real-time PCR quantitation of hepatitis B virus DNA using automated sample preparation and murine cytomegalovirus internal control

, 2005
Quantitation of circulating hepatitis B virus (HBV) DNA is important for monitoring disease progression and for assessing the response to antiviral therapy. Several commercial and 'in house' assays for HBV DNA quantitation have been described but many of
Tedder, RS, Ayliffe, U, Grant, PR, Ferns, RB, Garson, JA   +4 more
core  

Harnessing Phase Separation for the Development of High‐Performance Hydrogels

Advanced Science, EarlyView.
ABSTRACT Hydrogels are indispensable for the development of next‐generation bioelectronics, soft robotics, and biomedical devices, where their mechanical properties determine performance and reliability. Among strategies to enhance hydrogel mechanics, phase separation enables controlled heterogeneity resulting in gel networks that are reinforced by ...
Yue Shao, Yiming Ma, Baihao Shao, Molly M. Stevens   +3 more
wiley   +1 more source

A prospective evaluation of selection criteria for the use of Nucleic Acid Amplification Techniques to test for the presence of Mycobacterium tuberculosis in respiratory tract samples.

, 2009
Although Mycobacterium tuberculosis (MTB) can be detected rapidly by means of Nucleic Acid Amplification Techniques (NAT), these NAT tests are expensive and therefore are not used in routine practice or as a screening tool.Evaluation StudiesJournal ...
Scheen, Robert, Dediste, Anne, Vandenberg, Olivier, Van den Wijngaert, Sigi   +3 more
core  

Multidimensional and Multifunctional Laser‐Induced Graphene (LIG) for Point‐of‐Care and Wearable Biosensing, Theranostics, and Bioactive Interfaces Toward Personalized Healthcare and Regenerative Medicine

Advanced Science, EarlyView.
Multidimensional laser‐induced graphene (LIG) spanning from 0D to 3D architectures is comprehensively reviewed for multifunctional biomedical platforms, including biosensing, theranostics, and bioactive interface applications, which highlights its potentials for point‐of‐care diagnostics, wearable health monitoring, smart drug delivery, and tissue ...
Li Zhang, Yurou Yuan, Xiaohong Ding, Wing Cheung Mak   +3 more
wiley   +1 more source

Current and Future Perspectives on Isothermal Nucleic Acid Amplification Technologies for Diagnosing Infections

Infection and Drug Resistance, 2020
Godwin Attah Obande,1,2 Kirnpal Kaur Banga Singh1 1Department of Medical Microbiology & Parasitology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kelantan, Malaysia; 2Department of Microbiology, Faculty of Science, Federal ...
Obande GA, Banga Singh KK
doaj  

Enhanced Intracellular Stability and Translation Efficiency of mRNA Drugs by a 2‐arm mRNA Platform

Advanced Science, EarlyView.
We constructed a 2‐arm mRNA, characterized by a unique topology formed through the dimerization of two mRNA 3’ tails. The 2‐arm mRNA improves 3’ tail stability and resistance to nuclease degradation, resulting in an intracellular half‐life of up to 65 h. This method substantially enhances the translation capacity of mRNA drugs.
Xucong Teng, Jiahao Lin, Qiushuang Zhang, Xiangdong Zhang, Yicong Dai, Jinghong Li   +5 more
wiley   +1 more source

Enhancing ELISA Sensitivity: From Surface Engineering to Synthetic Biology

Biosensors
Accurate and sensitive detection of protein biomarkers is critical for advancing in vitro diagnostics (IVD), yet conventional enzyme-linked immunosorbent assays (ELISA) often fall short in terms of sensitivity compared to nucleic acid-based tests ...
Hye-Bin Jeon, Dong-Yeon Song, Yu Jin Park, Dong-Myung Kim   +3 more
doaj   +1 more source

DNA Nanoarray for Multiplexed RNA Detection With Single‐Molecule Readout

Advanced Science, EarlyView.
A carrier‐based DNA nanoarray integrates programmable nucleic acid hybridization with solid‐state nanopore readout for direct RNA detection at single‐molecule resolution. A ternary encoding system enables 27 carriers with 81 addressable sensing sites, allowing the simultaneous identification of targets spanning a wide size range, from short microRNAs ...
Yunxuan Li, Yesheng Wang, Chunmiao Yu, Siyu Hou, Elli Mylona, Plamena Naydenova, Jingyuan Zhao, Hong Yuan, Jingsong Duan, Hendrik F. P. Runge, Stephen Baker, Jinbo Zhu, Ulrich F. Keyser   +12 more
wiley   +1 more source

A bibliometric analysis of nucleic acid probe and its applications in oncology: towards more precise molecular medicine

Discover Oncology
Background Nucleic acid probes, which are short sequences of nucleic acids designed to complement specific DNA or RNA targets, have broad applications in biosensing, genetic studies, and various other fields.
Xingyu Zhong, Haodong He, Yifan Xiong, Jianxuan Sun, Na Zeng, Shaogang Wang, Qidong Xia   +6 more
doaj   +1 more source

Allosteric DNAzyme‐Enabled Sensitive and Multiplex Detection of Biomarkers for Rapid Diagnosis of Urinary Tract Infections

Advanced Science, EarlyView.
A catalytic allosteric DNAzyme assay (SMART) is developed by engineering DNAzyme into a unimolecular biosensor, enabling highly sensitive and multiplex detection of small molecules and nucleic acids. SMART enables extraction‐free, one‐pot, one‐step, isothermal, and cost‐effective detection of biological markers, achieving >95% accuracy for rapid ...
Yanzhe Shen, Chenzhi Shi, Xiaowei Ma, Pengfei Hou, Haomin Zhang, Juanxiu Qin, Li Pan, Guilin Li, Lifei Gao, Qian Ma, Donglei Yang, Min Li, Pengfei Wang   +12 more
wiley   +1 more source