Results 101 to 110 of about 78,833 (285)

A Parylene Real Time PCR Microdevice [PDF]

, 2010
The polymerase chain reaction (PCR) is a powerful biochemical assay that is used in virtually all biochemical labs. By specifically amplifying a small sample of DNA, this technique is useful in the fields of paternity testing, forensics, and virus ...
Quach, Quoc Chan (Brandon)
core   +1 more source

Inhibition of SIRT7 Overcomes Radioresistance in Pancreatic Neuroendocrine Tumors by Reactivating MEN1 Expression

Advanced Science, EarlyView.
Pancreatic neuroendocrine tumors frequently silence MEN1 through epigenetic mechanisms. Here, SIRT7 recruits DNMT1 to the MEN1 promoter, drives hypermethylation, and enhances DNA repair. Inhibiting SIRT7 restores MEN1, reduces MRN complex abundance, impairs double‐strand break repair, and sensitizes PanNET models to radiation, supporting SIRT7 as a ...
Jianyun Jiang, Yan Wang, Yi Qin, Luohai Chen, Xiaowu Xu, Guixiong Fan, Desheng Jing, Miaoyan Wei, Xianjun Yu, Junfeng Xu, Shunrong Ji, Jie Chen   +11 more
wiley   +1 more source

Research progress on application of novel isothermal amplification technology in waterborne pathogens detection

环境与职业医学
Water resources are crucial for environmental protection and the health of humans, plants, and animals. Contamination of water by pathogens such as bacteria, viruses, and protozoa can lead to outbreaks of various water-related infectious diseases, posing
Fubin HUANG, Eryi SHU, Hongliang FAN
doaj   +1 more source

Implantação dos testes de amplificação de ácidos nucléicos HIV/HCV Bio-Manguinhos na triagem de doadores de sangue: questões epidemiológicas e logísticas [PDF]

, 2013
Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências da Saúde, Programa de Pós-Graduação em Saúde Coletiva, Florianópolis, 2013.A questão da segurança transfusional relacionada à redução da transmissão do HIV e HCV está ligada ao
Petry, Andrea
core  

DNA extraction from formalin-fixed tissue: new light from the Deep-Sea

, 2010
DNA samples were extracted from ethanol and formalin-fixed decapod crustacean tissue using a new method based on Tetramethylsilane (TMS)-Chelex. It is shown that neither an indigestible matrix of cross-linked protein nor soluble PCR inhibitors impede PCR
Thatje, S., Johnston, J., Johnston, David, Mackenzie-Dodds, Jackie, Clark, P., Hall, S., Palero, F., Mackenzie-Dodds, J., Hall, Sally, Clark, Paul, Palero, Ferran, Mackenzie Dodds, Jackie, Clark, Paul F., Palero, Ferran ; https://orcid.org/, Thatje, Sven   +14 more
core   +1 more source

Machine‐Learning Microfluidic Minute‐Scale Microorganism Metrics Monitoring(M6)

Advanced Science, EarlyView.
ABSTRACT On‐site monitoring of microorganisms remains challenging because of low concentrations, strong background interference, and dynamic aerosol diffusion, particularly for aerosol‐transmitted pathogens. Here, we report a rapid detection platform that integrates a Puri‐focusing microfluidic chip, electrochemical impedance spectroscopy (EIS), and ...
Ning Yang, Jiahao Ding, Si Chen, Lijie Yan, Shichao Ding, Lavonda Li, Junyi Sun, Haodong Liu, Tongge Li, Ning Liu, Mingji Wei, Xiaoyong Zhu, Xiaobo Zou, Shouqi Yuan, Xingcai Zhang   +14 more
wiley   +1 more source

Programmable mesophilic argonaute-triggered SERS biosensor promotes amplification-free and multiplexed nucleic acid detection

Simultaneous on-site identification of multiple nucleic acids with rapidity and accuracy has consistently posed a significant challenge in the analytical chemistry field.
Wang, Xiaona, Wang, Yu, Wu, Di; id_orcid, Liu, Jianghua, Miao, Peize, Li, Guoliang, Shi, Yiheng, Meng, Yuxuan, Zhang, Ziyi   +8 more
core   +1 more source

DNA Replication Errors Drive Genome‐Wide Small Inverted Triplication Dynamics

Advanced Science, EarlyView.
This study provides insight into the dynamic equilibrium mechanism of a novel structural variant, small inverted triplication (SIT), which is generated by misalignment of the 3’ flap generated under DNA replication stress with palindromic sequence. Alternatively, the end sequence may fold back on itself to form an inverted fragment.
Yi Lei, Yu Zhou, Haitao Sun, Hang Yuan, Xinyu Pei, Jessica D. Hess, Yao Yan, Zunsong Hu, Mian Zhou, Zhaohui Gu, Li Zheng, Xiwei Wu, Binghui Shen   +12 more
wiley   +1 more source

Critical use of nucleic acid amplification techniques to test for Mycobacterium tuberculosis in respiratory tract samples.

, 2004
The usefulness of employing Belgian selection criteria before performing nucleic acid amplification techniques (NAT) was evaluated. The results of this study show that for smear-negative patients with an abnormal chest radiology result in the absence of ...
Gerard, Michel, Dediste, Anne, Zissis, Georges, Vandenberg, Olivier, Van den Wijngaert, Sigi, Vanlaethem, Yves   +5 more
core  

Engineering Approaches to Modify Immunomodulatory Functions of Mesenchymal Stromal Cells (MSCs): Tissue Regeneration and Clinical Application

Advanced Science, EarlyView.
Mesenchymal stromal cells (MSCs) show promise for treating immune‐related disorders through immunomodulation and tissue regeneration. This review gives a brief overview of current clinical approval of MSC therapies. It also discussed how bioengineering, including genetic modification, biomaterial delivery, extracellular vesicles, and iPSC‐derived MSCs,
Sichen Yang, Kejia Li, Ning Wang, Zhen Li, Zhiyong Zhang, Rocky S. Tuan, Yangzi Jiang   +6 more
wiley   +1 more source