Results 11 to 20 of about 26,794 (255)
Foods, 2022 The isothermal amplification method, a molecular-based diagnostic technology, such as loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA), is widely used as an alternative to the time-consuming and labor-intensive
Ye-Ji Moon, So-Young Lee, Se-Wook Oh
doaj +1 more source
Molecular Mechanisms Underlying CRISPR/Cas-Based Assays for Nucleic Acid Detection
Current Issues in Molecular Biology, 2023 Applied to investigate specific sequences, nucleic acid detection assays can help identify novel bacterial and viral infections. Most up-to-date systems combine isothermal amplification with Cas-mediated detection.
Denis N. Antropov, Grigory A. Stepanov
doaj +1 more source
Recent Uses of Paper Microfluidics in Isothermal Nucleic Acid Amplification Tests
Biosensors, 2023 Isothermal nucleic acid amplification tests have recently gained popularity over polymerase chain reaction (PCR), as they only require a constant temperature and significantly simplify nucleic acid amplification.
Jocelyn Reynolds +4 more
doaj +1 more source
Journal of Infection in Developing Countries, 2021 Approximately 1.41 million people die annually due to tuberculosis. One of the main problems in Tuberculosis eradication is the development of resistance to various antibiotics.
Muhammad Habiburrahman +2 more
doaj +1 more source
New Method of Isothermal, Hairpin Assisted, Primer Independent Amplification of DNA
BioChem, 2023 The isothermal amplification of nucleic acids refers to processes that quickly increase the amount of DNA at a constant temperature. These methods are mainly developed as alternatives to PCR for cases in which the application of a thermal cycler is not ...
Denis Sergeevich Naberezhnov +3 more
doaj +1 more source
Isothermal reactions for the amplification of oligonucleotides [PDF]
Proceedings of the National Academy of Sciences, 2003 We have devised a class of isothermal reactions for amplifying DNA. These homogeneous reactions rapidly synthesize short oligonucleotides (8–16 bases) specified by the sequence of an amplification template. Versions of the reactions can proceed in either a linear or an exponential amplification mode. Both of these reactions require
Jeffrey, Van Ness +2 more
openaire +2 more sources
BioTechniques, 2022 Coronavirus disease 2019 is a public health challenge requiring rapid testing for the detection of infections and transmission. Nucleic acid amplification tests targeting SARS coronavirus 2 (CoV2) are used to detect CoV2 in clinical samples.
Les Jones +3 more
doaj +1 more source
Animals, 2021 Over the years, development of molecular diagnostics has evolved significantly in the detection of pathogens within humans and their surroundings. Researchers have discovered new species and strains of viruses, while mitigating the viral infections that ...
Faiz Padzil +7 more
doaj +1 more source
Data in Brief, 2018 Loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) are two rapid isothermal amplification methods for detecting three common fungal root pathogens of cool-season turfgrass: Gaeumannomyces avenae, Magnaporthiopsis
Brijesh B. Karakkat +5 more
doaj +1 more source
Loop-mediated isothermal amplification of DNA [PDF]
Nucleic Acids Research, 2000 We have developed a novel method, termed loop-mediated isothermal amplification (LAMP), that amplifies DNA with high specificity, efficiency and rapidity under isothermal conditions. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA.
T, Notomi +6 more
openaire +2 more sources