Results 11 to 20 of about 388,328 (197)

Development of SARS-CoV-2 Isothermal Amplification Detection Kits

Nano Biomedicine and Engineering, 2020
The SARS-CoV-2 isothermal amplification detection kits based on loop-mediated isothermal amplification (LAMP) were developed and evaluated on three types samples of SARS-CoV-2. The kits included enzyme reaction mixtures and chromogenic agents.
Xueling Li, Jing Tian, Yan Xu, Xiaomin Chen, Zexi Liu, Hui Liang, Caihong Jin, Daxiang Cui   +7 more
doaj   +2 more sources

The use of Multiple Displacement Amplification to increase the detection and genotyping of Trypanosoma samples immobilised on FTA filters. [PDF]

, 2007
Whole genome amplification methods are a recently developed tool for amplifying DNA from limited template. We report its application in trypanosome infections, characterized by low parasitemias.
Sweeney, L., Turner, C.M.R., Morrison, L.J., Likeufack, A.C.L., Truc, P., Tait, A., McCormack, G., MacLeod, A.   +7 more
core   +8 more sources

Combining LAMP and Au-Nanoprobe to detect INH-RIF resistance accurately in tuberculosis: an evidence-based review

Journal of Infection in Developing Countries, 2021
Approximately 1.41 million people die annually due to tuberculosis. One of the main problems in Tuberculosis eradication is the development of resistance to various antibiotics.
Muhammad Habiburrahman, Haekal Ariq, Raden Rara Diah Handayani   +2 more
doaj   +1 more source

Isothermal reactions for the amplification of oligonucleotides [PDF]

Proceedings of the National Academy of Sciences, 2003
We have devised a class of isothermal reactions for amplifying DNA. These homogeneous reactions rapidly synthesize short oligonucleotides (8–16 bases) specified by the sequence of an amplification template. Versions of the reactions can proceed in either a linear or an exponential amplification mode. Both of these reactions require
Jeffrey, Van Ness, Lori K, Van Ness, David J, Galas   +2 more
openaire   +2 more sources

New Method of Isothermal, Hairpin Assisted, Primer Independent Amplification of DNA

BioChem, 2023
The isothermal amplification of nucleic acids refers to processes that quickly increase the amount of DNA at a constant temperature. These methods are mainly developed as alternatives to PCR for cases in which the application of a thermal cycler is not ...
Denis Sergeevich Naberezhnov, Alexander Andreevich Alferov, Yuriy Borisovich Kuzmin, Nikolay Evgenievich Kushlinskii   +3 more
doaj   +1 more source

Isothermal amplification using sequence-specific fluorescence detection of SARS coronavirus 2 and variants in nasal swabs

BioTechniques, 2022
Coronavirus disease 2019 is a public health challenge requiring rapid testing for the detection of infections and transmission. Nucleic acid amplification tests targeting SARS coronavirus 2 (CoV2) are used to detect CoV2 in clinical samples.
Les Jones, Hemant K Naikare, Yung-Yi C Mosley, Ralph A Tripp   +3 more
doaj   +1 more source

Loop-Mediated Isothermal Amplification (LAMP) as a Promising Point-of-Care Diagnostic Strategy in Avian Virus Research

Animals, 2021
Over the years, development of molecular diagnostics has evolved significantly in the detection of pathogens within humans and their surroundings. Researchers have discovered new species and strains of viruses, while mitigating the viral infections that ...
Faiz Padzil, Abdul Razak Mariatulqabtiah, Wen Siang Tan, Kok Lian Ho, Nurulfiza Mat Isa, Han Yih Lau, Jalila Abu, Kuo-Pin Chuang   +7 more
doaj   +1 more source

Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for detection of Leishmania DNA in buffy coat from visceral leishmaniasis patients [PDF]

, 2012
BACKGROUND: Visceral leishmaniasis (VL) remains as one of the most neglected tropical diseases with over 60% of the world's total VL cases occurring in the Indian subcontinent.
Mondal, D., Khondaker Rifat Hasan Bhaskar, Tania Akther, Gerd Pluschke, Bhaskar, Khondaker Rifat Hasan, Pluschke Gerd, Akther, Tania, Salam, M. A., Mondal, Dinesh, Khan, M. G., Khan Md Gulam Musawwir, Salam Md Abdus, Akther Tania, Dinesh Mondal, Pluschke, G., Md Abdus Salam, Khan, Md. Gulam Musawwir, Salam, Md. Abdus, Mondal Dinesh, Bhaskar Khondaker Rifat Hasan, Md Gulam Musawwir Khan, Akther, T., Pluschke, Gerd, Bhaskar, K. R.   +23 more
core   +1 more source

Data for designing two isothermal amplification assays for the detection of root-infecting fungi on cool-season turfgrasses

Data in Brief, 2018
Loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) are two rapid isothermal amplification methods for detecting three common fungal root pathogens of cool-season turfgrass: Gaeumannomyces avenae, Magnaporthiopsis
Brijesh B. Karakkat, Kurt Hockemeyer, Margot Franchett, Megan Olson, Cortney Mullenberg, Paul L. Koch   +5 more
doaj   +1 more source

Loop-mediated isothermal amplification of DNA [PDF]

Nucleic Acids Research, 2000
We have developed a novel method, termed loop-mediated isothermal amplification (LAMP), that amplifies DNA with high specificity, efficiency and rapidity under isothermal conditions. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA.
T, Notomi, H, Okayama, H, Masubuchi, T, Yonekawa, K, Watanabe, N, Amino, T, Hase   +6 more
openaire   +2 more sources