Results 11 to 20 of about 63,752 (305)

Loop-mediated isothermal amplification of DNA [PDF]

Nucleic Acids Research, 2000
We have developed a novel method, termed loop-mediated isothermal amplification (LAMP), that amplifies DNA with high specificity, efficiency and rapidity under isothermal conditions. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA.
T, Notomi, H, Okayama, H, Masubuchi, T, Yonekawa, K, Watanabe, N, Amino, T, Hase   +6 more
openaire   +4 more sources

Helicase‐dependent isothermal DNA amplification [PDF]

EMBO reports, 2004
Polymerase chain reaction is the most widely used method for in vitro DNA amplification. However, it requires thermocycling to separate two DNA strands. In vivo , DNA is replicated by DNA polymerases with various accessory proteins, including a DNA ...
Myriam, Vincent, Yan, Xu, Huimin, Kong
openaire   +2 more sources

Principles and Applications of Loop-Mediated Isothermal Amplification to Point-of-Care Tests

Biosensors, 2022
For the identification of nucleic acids, which are important biomarkers of pathogen-mediated diseases and viruses, the gold standard for NA-based diagnostic applications is polymerase chain reaction (PCR).
Jee-Woong Park
doaj   +1 more source

Combining LAMP and Au-Nanoprobe to detect INH-RIF resistance accurately in tuberculosis: an evidence-based review

Journal of Infection in Developing Countries, 2021
Approximately 1.41 million people die annually due to tuberculosis. One of the main problems in Tuberculosis eradication is the development of resistance to various antibiotics.
Muhammad Habiburrahman, Haekal Ariq, Raden Rara Diah Handayani   +2 more
doaj   +1 more source

Real-time kinetics and high-resolution melt curves in single-molecule digital LAMP to differentiate and study specific and non-specific amplification [PDF]

, 2020
Isothermal amplification assays, such as loop-mediated isothermal amplification (LAMP), show great utility for the development of rapid diagnostics for infectious diseases because they have high sensitivity, pathogen-specificity and potential for ...
Barlow, Jacob T., Ismagilov, Rustem F., Jue, Erik, Rolando, Justin C.   +3 more
core   +2 more sources

Ligation-free isothermal nucleic acid amplification

Biosensors and Bioelectronics, 2022
In this study, we uncover a ligation-free DNA extension method in two adjacent fragmented probes, which are hybridized to target RNA, for developing a ligation-free nucleic acid amplification reaction. In this reaction, DNA elongation occurs from a forward probe to a phosphorothioated-hairpin probe in the presence of target RNA regardless of ligation ...
Jeong Moon, Jayeon Song, Hyowon Jang, Hyunju Kang, Yong-Min Huh, Hye Young Son, Hyun Wook Rho, Mirae Park, Chandana S. Talwar, Kwang-Hyun Park, Euijeon Woo, Jaewoo Lim, Eun-Kyung Lim, Juyeon Jung, Yongwon Jung, Hyun Gyu Park, Taejoon Kang   +16 more
openaire   +3 more sources

New Method of Isothermal, Hairpin Assisted, Primer Independent Amplification of DNA

BioChem, 2023
The isothermal amplification of nucleic acids refers to processes that quickly increase the amount of DNA at a constant temperature. These methods are mainly developed as alternatives to PCR for cases in which the application of a thermal cycler is not ...
Denis Sergeevich Naberezhnov, Alexander Andreevich Alferov, Yuriy Borisovich Kuzmin, Nikolay Evgenievich Kushlinskii   +3 more
doaj   +1 more source

Isothermal amplification using sequence-specific fluorescence detection of SARS coronavirus 2 and variants in nasal swabs

BioTechniques, 2022
Coronavirus disease 2019 is a public health challenge requiring rapid testing for the detection of infections and transmission. Nucleic acid amplification tests targeting SARS coronavirus 2 (CoV2) are used to detect CoV2 in clinical samples.
Les Jones, Hemant K Naikare, Yung-Yi C Mosley, Ralph A Tripp   +3 more
doaj   +1 more source

Differential DNA accessibility to polymerase enables 30-minute phenotypic β-lactam antibiotic susceptibility testing of carbapenem-resistant Enterobacteriaceae [PDF]

, 2020
The rise in carbapenem-resistant Enterobacteriaceae (CRE) infections has created a global health emergency, underlining the critical need to develop faster diagnostics to treat swiftly and correctly.
Garner, Omai B., Ismagilov, Rustem F., Liaw, Eric J., Savela, Emily S., Schoepp, Nathan G., Winnett, Alexander   +5 more
core   +1 more source

Loop-Mediated Isothermal Amplification (LAMP) as a Promising Point-of-Care Diagnostic Strategy in Avian Virus Research

Animals, 2021
Over the years, development of molecular diagnostics has evolved significantly in the detection of pathogens within humans and their surroundings. Researchers have discovered new species and strains of viruses, while mitigating the viral infections that ...
Faiz Padzil, Abdul Razak Mariatulqabtiah, Wen Siang Tan, Kok Lian Ho, Nurulfiza Mat Isa, Han Yih Lau, Jalila Abu, Kuo-Pin Chuang   +7 more
doaj   +1 more source