Results 41 to 50 of about 63,752 (305)
Data in Brief, 2020 This article reports experimental data related to the research article entitled “Prevention of DNA multimerization using phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase” (R.R. Garafutdinov, A.R. Sakhabutdinova, M.
Ravil R. Garafutdinov +3 more
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Isothermal amplification-assisted diagnostics for COVID-19
Biosensors and Bioelectronics, 2022 The scenery of molecular diagnostics for infectious diseases is rapidly evolving to respond to the COVID-19 epidemic. The sensitivity and specificity of diagnostics, along with speed and accuracy, are crucial requirements for effective analytical tools to address the disease spreading around the world.
Mariarita De Felice +4 more
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Scientific Reports, 2017 Isothermal amplification methods for detection of DNA and RNA targets have expanded significantly in recent years, promising a new wave of simple and rapid molecular diagnostics. Current isothermal methods result in the generation of short fragments (
Yinhua Zhang, Nathan A. Tanner
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Molecular Methods for Identification and Quantification of Foodborne Pathogens
Molecules, 2022 Foodborne pathogens that enter the human food chain are a significant threat worldwide to human health. Timely and cost-effective detection of them became challenging for many countries that want to improve their detection and control of foodborne ...
Min Zhang +7 more
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Isothermal amplification method for next-generation sequencing [PDF]
Proceedings of the National Academy of Sciences, 2013 Significance We have developed a cost-effective in situ isothermal amplification method for next-generation sequencing (NGS) that can generate billions of monoclonal colonies in less than 30 minutes. This method can be used on NGS platforms to make the $1,000 genome possible and simplify the workflow for clinical genomic applications.
Zhaochun, Ma +7 more
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A Sensitive Potentiometric Sensor for Isothermal Amplification-Coupled Detection of Nucleic Acids
Sensors, 2018 A disposable potentiometric sensor was newly developed for the amplification-coupled detection of nucleic acids. The hydrogen-ion is generally released during isothermal amplification of nucleic acids.
Kang-Ho Lee +4 more
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Development of SARS-CoV-2 Isothermal Amplification Detection Kits
Nano Biomedicine and Engineering, 2020 The SARS-CoV-2 isothermal amplification detection kits based on loop-mediated isothermal amplification (LAMP) were developed and evaluated on three types samples of SARS-CoV-2. The kits included enzyme reaction mixtures and chromogenic agents.
Xueling Li +7 more
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Chemosensors, 2022 The determination of nucleic acids has become an analytical diagnostic method with many applications in fields such as biomedical sciences, environmental monitoring, forensic identification, and food safety.
Claudia Patiti +7 more
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The Lancet Global Health, 2021 Background: The detection and quantification of nucleic acids traditionally relies on PCR, which requires the use of expensive thermocyclers with integrated fluorescence detection of amplicons.
Jackson Coole, BS +6 more
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Electric LAMP: Virtual Loop-Mediated Isothermal AMPlification [PDF]
ISRN Bioinformatics, 2012 We present eLAMP, a PERL script, with Tk graphical interface, that electronically simulates Loop-mediated AMPlification (LAMP) allowing users to efficiently test putative LAMP primers on a set of target sequences. eLAMP can match primers to templates using either exact (via builtin PERL regular expressions) or approximate matching (via the tre-agrep ...
Salinas, Nelson R., Little, Damon P.
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