Results 41 to 50 of about 388,328 (197)

Chip-Based and Wearable Tools for Isothermal Amplification and Electrochemical Analysis of Nucleic Acids

Chemosensors, 2022
The determination of nucleic acids has become an analytical diagnostic method with many applications in fields such as biomedical sciences, environmental monitoring, forensic identification, and food safety.
Claudia Patiti, Patrick Severin Sfragano, Serena Laschi, Serena Pillozzi, Anna Boddi, Olivia Crociani, Andrea Bernini, Ilaria Palchetti   +7 more
doaj   +1 more source

Loop-mediated isothermal amplification (LAMP) method for rapid detection of Trypanosoma brucei rhodesiense [PDF]

, 2008
Loop-mediated isothermal amplification (LAMP) of DNA is a novel technique that rapidly amplifies target DNA under isothermal conditions. In the present study, a LAMP test was designed from the serum resistance-associated (SRA) gene of Trypanosoma brucei ...
Ndung'u, J.M., Njiru, Z.K., Enyaru, J.C., Thompson, R.C.A., Mikosza, A., Armstrong, T.   +5 more
core  

Real-time isothermal nucleic acid amplification detection in resource-limited settings: a description of an open-source miniature fluorimeter

The Lancet Global Health, 2021
Background: The detection and quantification of nucleic acids traditionally relies on PCR, which requires the use of expensive thermocyclers with integrated fluorescence detection of amplicons.
Jackson Coole, BS, Alex Kortum, MSc, Yubo Tang, PhD, Imran Vohra, BS, Mary Natoli, PhD, Kathryn Kundrod, PhD, Rebecca Richards-Kortum, PhD   +6 more
doaj   +1 more source

Isothermal Amplification of Rabies Virus Gene

Journal of Veterinary Medical Science, 2003
A sensitive and specific in situ amplification technique is needed to elucidate the dynamics of rabies virus in the body during the long incubation period after infection. To overcome the disadvantage of using the traditional reverse transcription (RT)-PCR in in situ studies, an isothermal nucleic acid sequence-based amplification (NASBA) technique was
SUGIYAMA, Makoto, ITO, Naoto, MINAMOTO, Nobuyuki   +2 more
openaire   +3 more sources

Loop-mediated isothermal amplification (LAMP) test for detection of Trypanosoma evansi strain B [PDF]

, 2010
Camel Trypanosomiasis (Surra) is mainly caused by Trypanosoma evansi strains that express variable surface glycoprotein (VSG) RoTat 1.2 However, in Kenya a second causative strain that does not express RoTat 1.2 VSG (T. evansi type B) has been identified.
Njiru, Z.K., Enyaru, J.C., Dargantes, A.P., Ouma, J.O.   +3 more
core  

The evolution of isothermal amplification technology: A bibliometric study (2013–2022)

Electronic Journal of Biotechnology
Background: Isothermal amplification is a nucleic acid amplification technology (NAAT) that has contributed significantly to molecular diagnostics. The combination of NAAT with a suitable detection platform has improved sensitivity and specificity and ...
Hanting Zhu, Zhitong Miao, Junfei Yuan, Wanying Xie, Qiaoqiao Zhang, Kun Yang   +5 more
doaj   +1 more source

Rapid on-site nucleic acid testing: On-chip sample preparation, amplification, and detection, and their integration into all-in-one systems

Frontiers in Bioengineering and Biotechnology, 2023
As nucleic acid testing is playing a vital role in increasingly many research fields, the need for rapid on-site testing methods is also increasing. The test procedure often consists of three steps: Sample preparation, amplification, and detection.
Jingwen Wang, Han Jiang, Leiming Pan, Xiuying Gu, Chaogeng Xiao, Pengpeng Liu, Yulong Tang, Jiehong Fang, Xiaoqian Li, Chenze Lu   +9 more
doaj   +1 more source

Current Trends in RNA Virus Detection via Nucleic Acid Isothermal Amplification-Based Platforms

Biosensors
Ribonucleic acid (RNA) viruses are one of the major classes of pathogens that cause human diseases. The conventional method to detect RNA viruses is real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), but it has some ...
Le Thi Nhu Ngoc, Young-Chul Lee
doaj   +1 more source

Probe-free label system for rapid detection of Cronobacter genus in powdered infant formula

AMB Express, 2018
Cronobacter species previously known as Enterobacter sakazakii poses high risks to neonates and infants. In this work a rapid detection method was developed which combined loop-mediated isothermal amplification with lateral flow assay for detection of ...
Shiqian Fu, Yujun Jiang, Xia Jiang, Yueming Zhao, Sihan Chen, Xinyan Yang, Chaoxin Man   +6 more
doaj   +1 more source

Polymerase-endonuclease amplification reaction for large-scale enzymatic production of antisense oligonucleotide [PDF]

, 2009
Synthetic oligonucleotides are contaminated with highly homologous failure sequences. Oligonucleotide synthesis is difficult to scale up because it requires expensive equipments, hazardous chemicals, and tedious purification process.
Deming Gou, Xiaolong Wang
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