Results 41 to 50 of about 33,911 (295)

Comparative analysis of methods for isothermal amplification of nucleic acids

Журнал микробиологии, эпидемиологии и иммунобиологии, 2022
In this review, methods for isothermal amplification of nucleic acids are considered and analyzed, in particular, loop isothermal amplification of DNA and RNA (LAMP/RT-LAMP), helicase-dependent amplification (HDA) and recombinase polymerase amplification
O. S. Chemisova, O. A. Tsyrulina, A. L. Trukhachev, A. K. Noskov   +3 more
doaj   +1 more source

Isothermal Amplification of Rabies Virus Gene

Journal of Veterinary Medical Science, 2003
A sensitive and specific in situ amplification technique is needed to elucidate the dynamics of rabies virus in the body during the long incubation period after infection. To overcome the disadvantage of using the traditional reverse transcription (RT)-PCR in in situ studies, an isothermal nucleic acid sequence-based amplification (NASBA) technique was
SUGIYAMA, Makoto, ITO, Naoto, MINAMOTO, Nobuyuki   +2 more
openaire   +3 more sources

Isothermal amplification-assisted diagnostics for COVID-19

Biosensors and Bioelectronics, 2022
The scenery of molecular diagnostics for infectious diseases is rapidly evolving to respond to the COVID-19 epidemic. The sensitivity and specificity of diagnostics, along with speed and accuracy, are crucial requirements for effective analytical tools to address the disease spreading around the world.
Mariarita De Felice, Mariarosaria De Falco, Daniele Zappi, Amina Antonacci, Viviana Scognamiglio   +4 more
openaire   +4 more sources

Field-Capable Genetic Amplification Module

, 2020
Field-Capable Loop-Mediated Isothermal Amplification ...
Alex Gorsuch, Gorsuch, Margaret, Lee, Jonathan, Harikumar, Sanjiv, Kannard-Smith, Mackie   +4 more
core   +1 more source

Real-time gene analysis based on a portable electrochemical microfluidic system

Electrochemistry Communications, 2020
Isothermal amplification, such as loop-mediated nucleic acid isothermal amplification (LAMP), is a highly promising technique that could revolutionize portable or point-of-care gene detection.
Yichen Liu, Baiyang Lu, Yidan Tang, Yan Du, Bingling Li   +4 more
doaj   +1 more source

Quantitative isothermal amplification on paper membranes using amplification nucleation site analysis [PDF]

Lab on a Chip, 2022
We report a novel method for the quantification of isothermal amplification reactions on paper membranes using discrete fluorescent amplification nucleation sites.
Benjamin P. Sullivan, Yu-Shan Chou, Andrew T. Bender, Coleman D. Martin, Zoe G. Kaputa, Hugh March, Minyung Song, Jonathan D. Posner   +7 more
openaire   +3 more sources

Assessing the presence of Wuchereria bancrofti in vector and human populations from urban communities in Conakry, Guinea [PDF]

, 2015
The Global Programme to Eliminate Lymphatic Filariasis was launched in 2000 with the goal of interrupting transmission of lymphatic filariasis (LF) through multiple rounds of mass drug administration (MDA).
Bockarie, Moses J., Bockarie, Moses, Utzinger, Jürg, Sandra A. King, Daniel A. Boakye, Andre Goepogui, Goepogui, Andre, Dziedzom K. de Souza, Benjamin G. Koudou, Narh, Charles A., Jürg Utzinger, Koudou, Benjamin, de Souza, Dziedzom K., Mamadou, Baldé S., Kouassi, Bernard L., King, Sandra A., Diakité, Lamia, Koudou, Benjamin G., Moses J. Bockarie, Boakye, Daniel A., Dadzie, Samuel K., Samuel K. Dadzie, Bernard L. Kouassi, Baldé S. Mamadou, Lamia Diakité, Charles A. Narh   +25 more
core   +1 more source

Rolling Circle and Loop Mediated Isothermal Amplification Strategy for Ultrasensitive miRNA Detection

Separations, 2021
Rolling circle amplification (RCA) and loop mediated isothermal amplification (LAMP) were combined to establish the rolling circle and loop mediated isothermal amplification (RC-LAMP) method for miRNA detection.
Zheng Cao, Xianfeng Jiang, Guizhou Xiao, Mingcheng Xu, Hui Liu, Sheng Cai   +5 more
doaj   +1 more source

Advances in Nucleic Acid Amplification-Based Microfluidic Devices for Clinical Microbial Detection

Chemosensors, 2022
Accurate and timely detection of infectious pathogens is urgently needed for disease treatment and control of possible outbreaks worldwide. Conventional methods for pathogen detection are usually time-consuming and labor-intensive.
Thi Ngoc Diep Trinh, Nae Yoon Lee
doaj   +1 more source

Isothermal nucleic acid amplification techniques: A comprehensive overview on their principle & applications as alternative to PCR

Practical Laboratory Medicine
Isothermal nucleic acid amplification methods have gained prominence as a valuable innovation in molecular diagnostics. These methods function at a constant temperature without the requirement of thermal cycler apparatus, simplifying the amplification ...
Alazar Amare Amdiyee, Tesfaye Sisay Tessema   +1 more
doaj   +1 more source