Results 51 to 60 of about 388,328 (197)

DNA Quality Assessment for Array CGH by Isothermal Whole Genome Amplification

Cellular Oncology, 2007
Background: Array Comparative Genomic Hybridization (array CGH) is increasingly applied on DNA obtained from formalin-fixed paraffin-embedded (FFPE) tissue, but in a proportion of cases this type of DNA is unsuitable.
Tineke E. Buffart, Marianne Tijssen, Thijs Krugers, Beatriz Carvalho, Serge J. Smeets, Ruud H. Brakenhoff, Heike Grabsch, Gerrit A. Meijer, Henry B. Sadowski, Bauke Ylstra   +9 more
doaj   +1 more source

Microfluidic Chip with Two-Stage Isothermal Amplification Method for Highly Sensitive Parallel Detection of SARS-CoV-2 and Measles Virus

Micromachines, 2021
A two-stage isothermal amplification method, which consists of a first-stage basic recombinase polymerase amplification (RPA) and a second-stage fluorescence loop-mediated isothermal amplification (LAMP), as well as a microfluidic-chip-based portable ...
Qin Huang, Xiaohui Shan, Ranran Cao, Xiangyu Jin, Xue Lin, Qiurong He, Yulei Zhu, Rongxin Fu, Wenli Du, Wenqi Lv, Ying Xia, Guoliang Huang   +11 more
doaj   +1 more source

A general solution for opening double-stranded DNA for isothermal amplification

, 2016
Nucleic acid amplification is the core technology of molecular biology and genetic engineering. Various isothermal amplification techniques have been developed as alternatives to polymerase chain reaction (PCR).
Li, N, Chen, GY, Huang, X, Yuan, Y, Cui, X, Tang, Z (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Peoples R China., Dong, J, Tang, Z   +7 more
core   +1 more source

Allele specific LAMP- gold nanoparticle for characterization of single nucleotide polymorphisms

Biotechnology Reports, 2017
Due to their relevance as disease biomarkers and for diagnostics, screening of single nucleotide polymorphism (SNPs) requires simple and straightforward strategies capable to provide results in medium throughput settings.
Fábio Ferreira Carlos, Bruno Veigas, Ana S. Matias, Gonçalo Doria, Orfeu Flores, Pedro V. Baptista   +5 more
doaj   +1 more source

Analysis of specific gene by integration of isothermal amplification and electrophoresis on poly(methyl methacrylate) microchips

, 2004
We have successfully achieved the integration of isothermal amplification and the subsequent analysis of specific gene fragments on poly(methyl methacrylate) microchips.
Notomi, T, Hataoka, Y, Baba, Y, Zhang, LH, Mori, Y, Tomita, N, 张丽华   +6 more
core   +1 more source

The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens

Plants, 2021
Rapid, sensitive, and timely diagnostics are essential for protecting plants from pathogens. Commonly, PCR techniques are used in laboratories for highly sensitive detection of DNA/RNA from viral, viroid, bacterial, and fungal pathogens of plants ...
Aleksandr V. Ivanov, Irina V. Safenkova, Anatoly V. Zherdev, Boris B. Dzantiev   +3 more
doaj   +1 more source

Isothermal Amplification and Multimerization of DNA by Bst DNA polymerase

, 2001
We have demonstrated the isothermal in vitro amplification and multimerization of several different linear DNA targets using only two primers and the strongly strand-displacing exonuclease-negative Bst DNA polymerase. This reaction has been termed linear
L.C. Wolter, Hafner, Gregory, Stafford, Mark, I.C. Yang, Giffard, Phillip, P.M. Giffard, Yang, Ilin, G.J. Hafner, M.R. Stafford, Wolter, Lyndsay   +9 more
core   +1 more source

Reverse Transcription Lesion-Induced DNA Amplification: An Instrument-Free Isothermal Method to Detect RNA [PDF]

, 2020
One challenge in point-of-care diagnostics is the lack of room-temperature methods for RNA detection based on enzymatic amplification and visualization steps.
Camilla, Mendes, Jesse, Yuzik, Yuning, Liu, Yimeng, Li, Bibi Safeenaz, Alladin-Mustan, Julianne, Gibbs, Daria Raquel, Queiroz de Almeida   +6 more
core   +2 more sources

Recent advances in recombinase polymerase amplification: Principle, advantages, disadvantages and applications

Frontiers in Cellular and Infection Microbiology, 2022
After the outbreak of SARS-CoV-2, nucleic acid testing quickly entered people’s lives. In addition to the polymerase chain reaction (PCR) which was commonly used in nucleic acid testing, isothermal amplification methods were also important nucleic acid ...
Meiying Tan, Chuan Liao, Lina Liang, Xueli Yi, Zihan Zhou, Guijiang Wei   +5 more
doaj   +1 more source

Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes

BioTechniques, 2015
Nucleic acid amplification is the basis for many molecular diagnostic assays. In these cases, the amplification product must be detected and analyzed, typically requiring extended workflow time, sophisticated equipment, or both.
Nathan A. Tanner, Yinhua Zhang, Thomas C. Evans   +2 more
doaj   +1 more source