Results 51 to 60 of about 33,911 (295)

Data on multimerization efficiency for short linear DNA templates and phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase

Data in Brief, 2020
This article reports experimental data related to the research article entitled “Prevention of DNA multimerization using phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase” (R.R. Garafutdinov, A.R. Sakhabutdinova, M.
Ravil R. Garafutdinov, Assol R. Sakhabutdinova, Maxim S. Kupryushkin, Dmitrii V. Pyshnyi   +3 more
doaj   +1 more source

Isothermal Amplification of Long, Discrete DNA Fragments Facilitated by Single-Stranded Binding Protein

Scientific Reports, 2017
Isothermal amplification methods for detection of DNA and RNA targets have expanded significantly in recent years, promising a new wave of simple and rapid molecular diagnostics. Current isothermal methods result in the generation of short fragments (
Yinhua Zhang, Nathan A. Tanner
doaj   +1 more source

A Sensitive Potentiometric Sensor for Isothermal Amplification-Coupled Detection of Nucleic Acids

Sensors, 2018
A disposable potentiometric sensor was newly developed for the amplification-coupled detection of nucleic acids. The hydrogen-ion is generally released during isothermal amplification of nucleic acids.
Kang-Ho Lee, Dongkyu Lee, Jongsu Yoon, Ohwon Kwon, Jaejong Lee   +4 more
doaj   +1 more source

Molecular Methods for Identification and Quantification of Foodborne Pathogens

Molecules, 2022
Foodborne pathogens that enter the human food chain are a significant threat worldwide to human health. Timely and cost-effective detection of them became challenging for many countries that want to improve their detection and control of foodborne ...
Min Zhang, Jiajia Wu, Zhaoai Shi, Aocheng Cao, Wensheng Fang, Dongdong Yan, Qiuxia Wang, Yuan Li   +7 more
doaj   +1 more source

A novel cassette method for probe evaluation in the designed biochips [PDF]

, 2013
A critical step in biochip design is the selection of probes with identical hybridisation characteristics. In this article we describe a novel method for evaluating DNA hybridisation probes, allowing the fine-tuning of biochips, that uses cassettes with ...
Julian Mitchell (572357), Irina Bogdarina, Abdulmohsen A. Al-Humam (572361), Nelly Sapojnikova (572356), Bogdarina, Irina, Vitaly Zinkevich, Sapojnikova, Nelly, Mitchell, Julian, Julian Mitchell, Al-humam, Abdulmohsen A., Nino Asatiani (572359), Zinkevich, Vitaly, Samia Alkhalil (572360), Abdulmohsen A Al-Humam, Nelly Sapojnikova, Irina Bogdarina (367051), Tamar Kartvelishvili, Vitaly Zinkevich (572355), Samia Alkhalil, Kartvelishvili, Tamar, Abdulmohsen A. Al-humam, Nino Asatiani, Alkhalil, Samia, Tamar Kartvelishvili (572358), Asatiani, Nino   +24 more
core   +1 more source

Chip-Based and Wearable Tools for Isothermal Amplification and Electrochemical Analysis of Nucleic Acids

Chemosensors, 2022
The determination of nucleic acids has become an analytical diagnostic method with many applications in fields such as biomedical sciences, environmental monitoring, forensic identification, and food safety.
Claudia Patiti, Patrick Severin Sfragano, Serena Laschi, Serena Pillozzi, Anna Boddi, Olivia Crociani, Andrea Bernini, Ilaria Palchetti   +7 more
doaj   +1 more source

An enhanced isothermal amplification assay for viral detection [PDF]

Nature Communications, 2020
Abstract Rapid, inexpensive, robust diagnostics are essential to control the spread of infectious diseases. Current state of the art diagnostics are highly sensitive and specific, but slow, and require expensive equipment. Here we report the development of a molecular diagnostic test for SARS-CoV-2 based on an enhanced recombinase ...
Jason Qian, Sarah A. Boswell, Christopher Chidley, Zhi-xiang Lu, Mary E. Pettit, Benjamin L. Gaudio, Jesse M. Fajnzylber, Ryan T. Ingram, Rebecca H. Ward, Jonathan Z. Li, Michael Springer   +10 more
openaire   +4 more sources

Real-time isothermal nucleic acid amplification detection in resource-limited settings: a description of an open-source miniature fluorimeter

The Lancet Global Health, 2021
Background: The detection and quantification of nucleic acids traditionally relies on PCR, which requires the use of expensive thermocyclers with integrated fluorescence detection of amplicons.
Jackson Coole, BS, Alex Kortum, MSc, Yubo Tang, PhD, Imran Vohra, BS, Mary Natoli, PhD, Kathryn Kundrod, PhD, Rebecca Richards-Kortum, PhD   +6 more
doaj   +1 more source

Isothermal amplification method for next-generation sequencing [PDF]

Proceedings of the National Academy of Sciences, 2013
Significance We have developed a cost-effective in situ isothermal amplification method for next-generation sequencing (NGS) that can generate billions of monoclonal colonies in less than 30 minutes. This method can be used on NGS platforms to make the $1,000 genome possible and simplify the workflow for clinical genomic applications.
Zhaochun, Ma, Raymond W, Lee, Bin, Li, Paul, Kenney, Yufang, Wang, Jonathan, Erikson, Swati, Goyal, Kaiqin, Lao   +7 more
openaire   +2 more sources

Enhancing Bubble Removal in Geometry‐Optimized Electrodes

Advanced Engineering Materials, EarlyView.
3D‐printed lattice electrodes outperform stochastic foams in alkaline water electrolysis despite 20%–25% lower surface area. Straight flow channels generate Venturi‐like bubble entrainment, suppressing gas accumulation that renders foam interiors electrochemically inactive.
Florian Wiesner, Peter Förster, Alexander Limper, Anselm Brodersen, Matthias Wessling, John Linkhorst   +5 more
wiley   +1 more source